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Protein

Protein-lysine deacetylase

Gene

dhaM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein deacetylase that removes acetyl groups on specific lysine residues in target proteins. Regulates transcription by catalyzing deacetylation of 'Lys-52' and 'Lys-62' of the transcriptional repressor RutR. Is also able to deacetylate NhoA and RluC in vitro. Targets a distinct set of substrates compared to CobB.1 Publication
Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.1 Publication

Cofactori

Note: Does not use NAD+ or Zn2+ like other established KDACs.1 Publication

Kineticsi

  1. KM=2.13 µM for acetylated RutR1 Publication
  1. Vmax=0.29 µmol/min/mg enzyme for the deacetylation of RutR1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91Tele-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication
Active sitei169 – 1691Pros-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication
Active sitei200 – 2001Nucleophile; for deacetylase activity1 Publication
Active sitei430 – 4301Tele-phosphohistidine intermediate1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG12399-MONOMER.
ECOL316407:JW5185-MONOMER.
MetaCyc:EG12399-MONOMER.
BRENDAi2.7.1.29. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-lysine deacetylase1 Publication (EC:3.5.1.-1 Publication)
Alternative name(s):
Dihydroxyacetone kinase subunit M1 Publication
Lysine deacetylase1 Publication
Short name:
KDAC1 Publication
PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM1 Publication
Gene namesi
Name:dhaM1 Publication
Synonyms:ycgC
Ordered Locus Names:b1198, JW5185
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12399. dhaM.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • integral component of membrane Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi9 – 91H → A: Loss of phosphotransfer activity. 1 Publication
Mutagenesisi169 – 1691H → A: Loss of phosphotransfer activity. 1 Publication
Mutagenesisi200 – 2001S → A: Loss of lysine deacetylase activity. 1 Publication
Mutagenesisi430 – 4301H → A: Loss of phosphotransfer activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Protein-lysine deacetylasePRO_0000186713Add
BLAST

Proteomic databases

PaxDbiP37349.
PRIDEiP37349.

2D gel databases

SWISS-2DPAGEP37349.

Interactioni

Subunit structurei

Homodimer (PubMed:11350937). The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.1 Publication

Protein-protein interaction databases

BioGridi4260761. 17 interactions.
DIPiDIP-11551N.
IntActiP37349. 1 interaction.
STRINGi511145.b1198.

Structurei

3D structure databases

ProteinModelPortaliP37349.
SMRiP37349. Positions 3-127, 156-241, 247-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 135135PTS EIIA type-4PROSITE-ProRule annotation1 PublicationAdd
BLAST
Domaini155 – 24288HPrPROSITE-ProRule annotation1 PublicationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni264 – 472209PTS EI-like, N-terminal part1 PublicationAdd
BLAST

Domaini

Consists of three domains. The N-terminal dimerization domain has the same fold as the IIA domain of the mannose transporter of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS). The middle domain is similar to HPr and the C-terminus is similar to the N-terminal domain of enzyme I (EI) of the PTS. The IIA domain of DhaM (via phospho-His-9), instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). The phosphoryl flow likely involves HPr ('His-15') -> DhaM (His-430 -> His-169 -> His-9) -> DhaL-ADP -> Dha. The HPr-like domain of DhaM cannot efficiently substitute for the general carrier protein HPr.1 Publication

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated
Contains 1 HPr domain.PROSITE-ProRule annotation
Contains 1 PTS EIIA type-4 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VDH. Bacteria.
COG1080. LUCA.
COG1925. LUCA.
COG3412. LUCA.
HOGENOMiHOG000119746.
KOiK05881.
OMAiTDHVLVM.
PhylomeDBiP37349.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di1.10.274.10. 1 hit.
3.30.1340.10. 1 hit.
3.40.50.510. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR012844. DhaM_N.
IPR000032. HPr-like.
IPR008279. PEP-util_enz_mobile_dom.
IPR004701. PTS_EIIA_man-typ.
IPR008731. PTS_EIN.
IPR001020. PTS_HPr_His_P_site.
[Graphical view]
PfamiPF03610. EIIA-man. 1 hit.
PF05524. PEP-utilisers_N. 1 hit.
PF00391. PEP-utilizers. 1 hit.
PF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF47831. SSF47831. 1 hit.
SSF52009. SSF52009. 1 hit.
SSF53062. SSF53062. 1 hit.
SSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR02364. dha_pts. 1 hit.
TIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51096. PTS_EIIA_TYPE_4. 1 hit.
PS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37349-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNLVIVSHS SRLGEGVGEL ARQMLMSDSC KIAIAAGIDD PQNPIGTDAV
60 70 80 90 100
KVMEAIESVA DADHVLVMMD MGSALLSAET ALELLAPEIA AKVRLCAAPL
110 120 130 140 150
VEGTLAATVS AASGADIDKV IFDAMHALEA KREQLGLPSS DTEISDTCPA
160 170 180 190 200
YDEEARSLAV VIKNRNGLHV RPASRLVYTL STFNADMLLE KNGKCVTPES
210 220 230 240 250
INQIALLQVR YNDTLRLIAK GPEAEEALIA FRQLAEDNFG ETEEVAPPTL
260 270 280 290 300
RPVPPVSGKA FYYQPVLCTV QAKSTLTVEE EQDRLRQAID FTLLDLMTLT
310 320 330 340 350
AKAEASGLDD IAAIFSGHHT LLDDPELLAA ASELLQHEHC TAEYAWQQVL
360 370 380 390 400
KELSQQYQQL DDEYLQARYI DVDDLLHRTL VHLTQTKEEL PQFNSPTILL
410 420 430 440 450
AENIYPSTVL QLDPAVVKGI CLSAGSPVSH SALIARELGI GWICQQGEKL
460 470
YAIQPEETLT LDVKTQRFNR QG
Length:472
Mass (Da):51,449
Last modified:November 24, 2009 - v3
Checksum:i7CEBEFBA1544C65E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74282.4.
AP009048 Genomic DNA. Translation: BAA36055.1.
X15868 Genomic DNA. No translation available.
PIRiC64866.
RefSeqiNP_415716.4. NC_000913.3.
WP_001301101.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74282; AAC74282; b1198.
BAA36055; BAA36055; BAA36055.
GeneIDi945749.
KEGGiecj:JW5185.
eco:b1198.
PATRICi32117646. VBIEscCol129921_1245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74282.4.
AP009048 Genomic DNA. Translation: BAA36055.1.
X15868 Genomic DNA. No translation available.
PIRiC64866.
RefSeqiNP_415716.4. NC_000913.3.
WP_001301101.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37349.
SMRiP37349. Positions 3-127, 156-241, 247-471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260761. 17 interactions.
DIPiDIP-11551N.
IntActiP37349. 1 interaction.
STRINGi511145.b1198.

2D gel databases

SWISS-2DPAGEP37349.

Proteomic databases

PaxDbiP37349.
PRIDEiP37349.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74282; AAC74282; b1198.
BAA36055; BAA36055; BAA36055.
GeneIDi945749.
KEGGiecj:JW5185.
eco:b1198.
PATRICi32117646. VBIEscCol129921_1245.

Organism-specific databases

EchoBASEiEB2299.
EcoGeneiEG12399. dhaM.

Phylogenomic databases

eggNOGiENOG4105VDH. Bacteria.
COG1080. LUCA.
COG1925. LUCA.
COG3412. LUCA.
HOGENOMiHOG000119746.
KOiK05881.
OMAiTDHVLVM.
PhylomeDBiP37349.

Enzyme and pathway databases

BioCyciEcoCyc:EG12399-MONOMER.
ECOL316407:JW5185-MONOMER.
MetaCyc:EG12399-MONOMER.
BRENDAi2.7.1.29. 2026.

Miscellaneous databases

PROiP37349.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di1.10.274.10. 1 hit.
3.30.1340.10. 1 hit.
3.40.50.510. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR012844. DhaM_N.
IPR000032. HPr-like.
IPR008279. PEP-util_enz_mobile_dom.
IPR004701. PTS_EIIA_man-typ.
IPR008731. PTS_EIN.
IPR001020. PTS_HPr_His_P_site.
[Graphical view]
PfamiPF03610. EIIA-man. 1 hit.
PF05524. PEP-utilisers_N. 1 hit.
PF00391. PEP-utilizers. 1 hit.
PF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF47831. SSF47831. 1 hit.
SSF52009. SSF52009. 1 hit.
SSF53062. SSF53062. 1 hit.
SSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR02364. dha_pts. 1 hit.
TIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51096. PTS_EIIA_TYPE_4. 1 hit.
PS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHAM_ECOLI
AccessioniPrimary (citable) accession number: P37349
Secondary accession number(s): P76013
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 24, 2009
Last modified: September 7, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKLM has no transport activity.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.