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Protein

Psp operon transcriptional activator

Gene

pspF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene.3 Publications

Enzyme regulationi

ATPase activity is inhibited by interaction with PspA. Under inducing conditions, the interaction is disrupted, allowing activation of psp transcription.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPPROSITE-ProRule annotation8
Nucleotide bindingi99 – 108ATPPROSITE-ProRule annotation10
DNA bindingi302 – 321H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12344-MONOMER.
ECOL316407:JW1296-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Psp operon transcriptional activator
Alternative name(s):
Phage shock protein F
Gene namesi
Name:pspF
Synonyms:ycjB
Ordered Locus Names:b1303, JW1296
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12344. pspF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000813291 – 325Psp operon transcriptional activatorAdd BLAST325

Proteomic databases

PaxDbiP37344.
PRIDEiP37344.

Interactioni

Subunit structurei

Forms a complex with PspA, which is composed of around 6 PspF subunits and 6 PspA subunits.1 Publication

Protein-protein interaction databases

BioGridi4263525. 650 interactors.
DIPiDIP-10592N.
IntActiP37344. 3 interactors.
STRINGi511145.b1303.

Structurei

Secondary structure

1325
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 25Combined sources13
Beta strandi32 – 35Combined sources4
Helixi42 – 51Combined sources10
Turni54 – 57Combined sources4
Beta strandi60 – 64Combined sources5
Turni65 – 67Combined sources3
Helixi70 – 78Combined sources9
Helixi95 – 98Combined sources4
Turni99 – 101Combined sources3
Beta strandi102 – 108Combined sources7
Helixi109 – 111Combined sources3
Helixi114 – 126Combined sources13
Beta strandi127 – 129Combined sources3
Beta strandi132 – 135Combined sources4
Beta strandi143 – 148Combined sources6
Helixi152 – 158Combined sources7
Helixi163 – 169Combined sources7
Beta strandi171 – 175Combined sources5
Helixi179 – 181Combined sources3
Helixi183 – 200Combined sources18
Helixi211 – 219Combined sources9
Helixi225 – 240Combined sources16
Beta strandi243 – 245Combined sources3
Turni254 – 256Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BJVX-ray1.70A1-265[»]
2BJWX-ray1.75A1-265[»]
2C96X-ray1.80A1-265[»]
2C98X-ray1.90A1-265[»]
2C99X-ray1.90A1-265[»]
2C9CX-ray2.10A1-265[»]
2VIIX-ray2.85A1-259[»]
4QNMX-ray1.63A1-265[»]
4QNRX-ray1.54A1-265[»]
4QOSX-ray1.42A1-265[»]
ProteinModelPortaliP37344.
SMRiP37344.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37344.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 237Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST223

Sequence similaritiesi

Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105E51. Bacteria.
COG1221. LUCA.
HOGENOMiHOG000058487.
InParanoidiP37344.
KOiK03974.
OMAiQTEKYEM.
PhylomeDBiP37344.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
IPR014317. Transcription_activator_PspF.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02974. phageshock_pspF. 1 hit.
PROSITEiPS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYKDNLLG EANSFLEVLE QVSHLAPLDK PVLIIGERGT GKELIASRLH
60 70 80 90 100
YLSSRWQGPF ISLNCAALNE NLLDSELFGH EAGAFTGAQK RHPGRFERAD
110 120 130 140 150
GGTLFLDELA TAPMMVQEKL LRVIEYGELE RVGGSQPLQV NVRLVCATNA
160 170 180 190 200
DLPAMVNEGT FRADLLDRLA FDVVQLPPLR ERESDIMLMA EYFAIQMCRE
210 220 230 240 250
IKLPLFPGFT ERARETLLNY RWPGNIRELK NVVERSVYRH GTSDYPLDDI
260 270 280 290 300
IIDPFKRRPP EDAIAVSETT SLPTLPLDLR EFQMQQEKEL LQLSLQQGKY
310 320
NQKRAAELLG LTYHQFRALL KKHQI
Length:325
Mass (Da):36,986
Last modified:October 1, 1996 - v2
Checksum:iF495D634193A42FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38542 Genomic DNA. Translation: AAB02186.1.
U00096 Genomic DNA. Translation: AAC74385.2.
AP009048 Genomic DNA. Translation: BAA14872.1.
X57560 Genomic DNA. No translation available.
PIRiB64879.
RefSeqiNP_415819.4. NC_000913.3.
WP_001301108.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74385; AAC74385; b1303.
BAA14872; BAA14872; BAA14872.
GeneIDi945683.
KEGGiecj:JW1296.
eco:b1303.
PATRICi32117878. VBIEscCol129921_1359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38542 Genomic DNA. Translation: AAB02186.1.
U00096 Genomic DNA. Translation: AAC74385.2.
AP009048 Genomic DNA. Translation: BAA14872.1.
X57560 Genomic DNA. No translation available.
PIRiB64879.
RefSeqiNP_415819.4. NC_000913.3.
WP_001301108.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BJVX-ray1.70A1-265[»]
2BJWX-ray1.75A1-265[»]
2C96X-ray1.80A1-265[»]
2C98X-ray1.90A1-265[»]
2C99X-ray1.90A1-265[»]
2C9CX-ray2.10A1-265[»]
2VIIX-ray2.85A1-259[»]
4QNMX-ray1.63A1-265[»]
4QNRX-ray1.54A1-265[»]
4QOSX-ray1.42A1-265[»]
ProteinModelPortaliP37344.
SMRiP37344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263525. 650 interactors.
DIPiDIP-10592N.
IntActiP37344. 3 interactors.
STRINGi511145.b1303.

Proteomic databases

PaxDbiP37344.
PRIDEiP37344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74385; AAC74385; b1303.
BAA14872; BAA14872; BAA14872.
GeneIDi945683.
KEGGiecj:JW1296.
eco:b1303.
PATRICi32117878. VBIEscCol129921_1359.

Organism-specific databases

EchoBASEiEB2248.
EcoGeneiEG12344. pspF.

Phylogenomic databases

eggNOGiENOG4105E51. Bacteria.
COG1221. LUCA.
HOGENOMiHOG000058487.
InParanoidiP37344.
KOiK03974.
OMAiQTEKYEM.
PhylomeDBiP37344.

Enzyme and pathway databases

BioCyciEcoCyc:EG12344-MONOMER.
ECOL316407:JW1296-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37344.
PROiP37344.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
IPR014317. Transcription_activator_PspF.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02974. phageshock_pspF. 1 hit.
PROSITEiPS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSPF_ECOLI
AccessioniPrimary (citable) accession number: P37344
Secondary accession number(s): P76039
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.