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Protein

Psp operon transcriptional activator

Gene

pspF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene.3 Publications

Enzyme regulationi

ATPase activity is inhibited by interaction with PspA. Under inducing conditions, the interaction is disrupted, allowing activation of psp transcription.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438ATPPROSITE-ProRule annotation
Nucleotide bindingi99 – 10810ATPPROSITE-ProRule annotation
DNA bindingi302 – 32120H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12344-MONOMER.
ECOL316407:JW1296-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Psp operon transcriptional activator
Alternative name(s):
Phage shock protein F
Gene namesi
Name:pspF
Synonyms:ycjB
Ordered Locus Names:b1303, JW1296
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12344. pspF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Psp operon transcriptional activatorPRO_0000081329Add
BLAST

Proteomic databases

PaxDbiP37344.
PRIDEiP37344.

Interactioni

Subunit structurei

Forms a complex with PspA, which is composed of around 6 PspF subunits and 6 PspA subunits.1 Publication

Protein-protein interaction databases

BioGridi4263525. 650 interactions.
DIPiDIP-10592N.
IntActiP37344. 3 interactions.
STRINGi511145.b1303.

Structurei

Secondary structure

1
325
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2513Combined sources
Beta strandi32 – 354Combined sources
Helixi42 – 5110Combined sources
Turni54 – 574Combined sources
Beta strandi60 – 645Combined sources
Turni65 – 673Combined sources
Helixi70 – 789Combined sources
Helixi95 – 984Combined sources
Turni99 – 1013Combined sources
Beta strandi102 – 1087Combined sources
Helixi109 – 1113Combined sources
Helixi114 – 12613Combined sources
Beta strandi127 – 1293Combined sources
Beta strandi132 – 1354Combined sources
Beta strandi143 – 1486Combined sources
Helixi152 – 1587Combined sources
Helixi163 – 1697Combined sources
Beta strandi171 – 1755Combined sources
Helixi179 – 1813Combined sources
Helixi183 – 20018Combined sources
Helixi211 – 2199Combined sources
Helixi225 – 24016Combined sources
Beta strandi243 – 2453Combined sources
Turni254 – 2563Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BJVX-ray1.70A1-265[»]
2BJWX-ray1.75A1-265[»]
2C96X-ray1.80A1-265[»]
2C98X-ray1.90A1-265[»]
2C99X-ray1.90A1-265[»]
2C9CX-ray2.10A1-265[»]
2VIIX-ray2.85A1-259[»]
4QNMX-ray1.63A1-265[»]
4QNRX-ray1.54A1-265[»]
4QOSX-ray1.42A1-265[»]
ProteinModelPortaliP37344.
SMRiP37344. Positions 7-322.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37344.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 237223Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105E51. Bacteria.
COG1221. LUCA.
HOGENOMiHOG000058487.
InParanoidiP37344.
KOiK03974.
OMAiQTEKYEM.
PhylomeDBiP37344.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
IPR014317. Transcription_activator_PspF.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02974. phageshock_pspF. 1 hit.
PROSITEiPS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYKDNLLG EANSFLEVLE QVSHLAPLDK PVLIIGERGT GKELIASRLH
60 70 80 90 100
YLSSRWQGPF ISLNCAALNE NLLDSELFGH EAGAFTGAQK RHPGRFERAD
110 120 130 140 150
GGTLFLDELA TAPMMVQEKL LRVIEYGELE RVGGSQPLQV NVRLVCATNA
160 170 180 190 200
DLPAMVNEGT FRADLLDRLA FDVVQLPPLR ERESDIMLMA EYFAIQMCRE
210 220 230 240 250
IKLPLFPGFT ERARETLLNY RWPGNIRELK NVVERSVYRH GTSDYPLDDI
260 270 280 290 300
IIDPFKRRPP EDAIAVSETT SLPTLPLDLR EFQMQQEKEL LQLSLQQGKY
310 320
NQKRAAELLG LTYHQFRALL KKHQI
Length:325
Mass (Da):36,986
Last modified:October 1, 1996 - v2
Checksum:iF495D634193A42FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38542 Genomic DNA. Translation: AAB02186.1.
U00096 Genomic DNA. Translation: AAC74385.2.
AP009048 Genomic DNA. Translation: BAA14872.1.
X57560 Genomic DNA. No translation available.
PIRiB64879.
RefSeqiNP_415819.4. NC_000913.3.
WP_001301108.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74385; AAC74385; b1303.
BAA14872; BAA14872; BAA14872.
GeneIDi945683.
KEGGiecj:JW1296.
eco:b1303.
PATRICi32117878. VBIEscCol129921_1359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38542 Genomic DNA. Translation: AAB02186.1.
U00096 Genomic DNA. Translation: AAC74385.2.
AP009048 Genomic DNA. Translation: BAA14872.1.
X57560 Genomic DNA. No translation available.
PIRiB64879.
RefSeqiNP_415819.4. NC_000913.3.
WP_001301108.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BJVX-ray1.70A1-265[»]
2BJWX-ray1.75A1-265[»]
2C96X-ray1.80A1-265[»]
2C98X-ray1.90A1-265[»]
2C99X-ray1.90A1-265[»]
2C9CX-ray2.10A1-265[»]
2VIIX-ray2.85A1-259[»]
4QNMX-ray1.63A1-265[»]
4QNRX-ray1.54A1-265[»]
4QOSX-ray1.42A1-265[»]
ProteinModelPortaliP37344.
SMRiP37344. Positions 7-322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263525. 650 interactions.
DIPiDIP-10592N.
IntActiP37344. 3 interactions.
STRINGi511145.b1303.

Proteomic databases

PaxDbiP37344.
PRIDEiP37344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74385; AAC74385; b1303.
BAA14872; BAA14872; BAA14872.
GeneIDi945683.
KEGGiecj:JW1296.
eco:b1303.
PATRICi32117878. VBIEscCol129921_1359.

Organism-specific databases

EchoBASEiEB2248.
EcoGeneiEG12344. pspF.

Phylogenomic databases

eggNOGiENOG4105E51. Bacteria.
COG1221. LUCA.
HOGENOMiHOG000058487.
InParanoidiP37344.
KOiK03974.
OMAiQTEKYEM.
PhylomeDBiP37344.

Enzyme and pathway databases

BioCyciEcoCyc:EG12344-MONOMER.
ECOL316407:JW1296-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37344.
PROiP37344.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
IPR014317. Transcription_activator_PspF.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02974. phageshock_pspF. 1 hit.
PROSITEiPS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSPF_ECOLI
AccessioniPrimary (citable) accession number: P37344
Secondary accession number(s): P76039
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.