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Protein

Multidrug resistance protein MdtK

Gene

mdtK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multidrug efflux pump that probably functions as a Na+/drug antiporter. Confers resistance to many drugs such as fluoroquinolones (norfloxacin, ciprofloxacin, enoxacin) and tetraphenylphosphonium ion (TPP) (PubMed:9661020, PubMed:11566977). Also to deoxycholate, doxorubicin, trimethoprim, chloramphenicol, fosfomycin, ethidium bromide and benzalkonium (PubMed:11566977). Also able to export peptides; when overexpressed, allows cells deleted for multiple peptidases (pepA, pepB, pepD and pepN) to grow in the presence of dipeptides Ala-Gln or Gly-Tyr which otherwise inhibit growth (PubMed:20067529). Cells overexpressing this protein have decreased intracellular levels of Ala-Gln dipeptide, and in a system that produces the Ala-Gln dipeptide overproduction of this protein increases export of the dipeptide (PubMed:20067529).4 Publications

GO - Molecular functioni

  • antibiotic transporter activity Source: EcoCyc
  • antiporter activity Source: UniProtKB-HAMAP
  • dipeptide transmembrane transporter activity Source: EcoCyc
  • drug transmembrane transporter activity Source: EcoliWiki
  • transporter activity Source: EcoliWiki

GO - Biological processi

  • antibiotic transport Source: EcoCyc
  • cellular response to reactive oxygen species Source: EcoCyc
  • dipeptide transmembrane transport Source: EcoCyc
  • response to antibiotic Source: EcoCyc
  • sodium ion transport Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Antiport, Ion transport, Peptide transport, Protein transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

BioCyciEcoCyc:YDHE-MONOMER.
ECOL316407:JW1655-MONOMER.

Protein family/group databases

TCDBi2.A.66.1.3. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein MdtK
Alternative name(s):
Multidrug-efflux transporter
Gene namesi
Name:mdtK
Synonyms:norE1 Publication, norM1 Publication, ydhE
Ordered Locus Names:b1663, JW1655
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12400. mdtK.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence analysis
Transmembranei11 – 3121HelicalSequence analysisAdd
BLAST
Topological domaini32 – 5221PeriplasmicSequence analysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence analysisAdd
BLAST
Topological domaini74 – 9219CytoplasmicSequence analysisAdd
BLAST
Transmembranei93 – 11321HelicalSequence analysisAdd
BLAST
Topological domaini114 – 12613PeriplasmicSequence analysisAdd
BLAST
Transmembranei127 – 14721HelicalSequence analysisAdd
BLAST
Topological domaini148 – 15912CytoplasmicSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Topological domaini181 – 1888PeriplasmicSequence analysis
Transmembranei189 – 20921HelicalSequence analysisAdd
BLAST
Topological domaini210 – 24233CytoplasmicSequence analysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence analysisAdd
BLAST
Topological domaini264 – 27512PeriplasmicSequence analysisAdd
BLAST
Transmembranei276 – 29621HelicalSequence analysisAdd
BLAST
Topological domaini297 – 31317CytoplasmicSequence analysisAdd
BLAST
Transmembranei314 – 33421HelicalSequence analysisAdd
BLAST
Topological domaini335 – 34915PeriplasmicSequence analysisAdd
BLAST
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST
Topological domaini371 – 38616CytoplasmicSequence analysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence analysisAdd
BLAST
Topological domaini408 – 41710PeriplasmicSequence analysis
Transmembranei418 – 43821HelicalSequence analysisAdd
BLAST
Topological domaini439 – 45719Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1681613.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Multidrug resistance protein MdtKPRO_0000164181Add
BLAST

Proteomic databases

PaxDbiP37340.
PRIDEiP37340.

Interactioni

Protein-protein interaction databases

BioGridi4260264. 200 interactions.
STRINGi511145.b1663.

Chemistry

BindingDBiP37340.

Structurei

3D structure databases

ProteinModelPortaliP37340.
SMRiP37340. Positions 1-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QI1. Bacteria.
COG0534. LUCA.
HOGENOMiHOG000038538.
InParanoidiP37340.
KOiK03327.
OMAiSIVFMLP.
PhylomeDBiP37340.

Family and domain databases

HAMAPiMF_00400. MdtK. 1 hit.
InterProiIPR002528. MATE_fam.
IPR022913. Multidrug-R_MdtK.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
PIRSFiPIRSF006603. DinF. 1 hit.
TIGRFAMsiTIGR00797. matE. 1 hit.

Sequencei

Sequence statusi: Complete.

P37340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKYISEARL LLALAIPVIL AQIAQTAMGF VDTVMAGGYS ATDMAAVAIG
60 70 80 90 100
TSIWLPAILF GHGLLLALTP VIAQLNGSGR RERIAHQVRQ GFWLAGFVSV
110 120 130 140 150
LIMLVLWNAG YIIRSMENID PALADKAVGY LRALLWGAPG YLFFQVARNQ
160 170 180 190 200
CEGLAKTKPG MVMGFIGLLV NIPVNYIFIY GHFGMPELGG VGCGVATAAV
210 220 230 240 250
YWVMFLAMVS YIKRARSMRD IRNEKGTAKP DPAVMKRLIQ LGLPIALALF
260 270 280 290 300
FEVTLFAVVA LLVSPLGIVD VAGHQIALNF SSLMFVLPMS LAAAVTIRVG
310 320 330 340 350
YRLGQGSTLD AQTAARTGLM VGVCMATLTA IFTVSLREQI ALLYNDNPEV
360 370 380 390 400
VTLAAHLMLL AAVYQISDSI QVIGSGILRG YKDTRSIFYI TFTAYWVLGL
410 420 430 440 450
PSGYILALTD LVVEPMGPAG FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI

ILQRASR
Length:457
Mass (Da):49,447
Last modified:November 1, 1997 - v2
Checksum:i33A60FC42ED6CFD7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321D → S in AAB47941 (PubMed:9023191).Curated
Sequence conflicti100 – 1001V → A in X69109 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68703 Genomic DNA. Translation: AAB47941.1.
U00096 Genomic DNA. Translation: AAT48136.1.
AP009048 Genomic DNA. Translation: BAA15430.1.
X69109 Genomic DNA. No translation available.
PIRiA64924.
RefSeqiWP_001174940.1. NZ_LN832404.1.
YP_025307.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48136; AAT48136; b1663.
BAA15430; BAA15430; BAA15430.
GeneIDi945883.
KEGGiecj:JW1655.
eco:b1663.
PATRICi32118632. VBIEscCol129921_1736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68703 Genomic DNA. Translation: AAB47941.1.
U00096 Genomic DNA. Translation: AAT48136.1.
AP009048 Genomic DNA. Translation: BAA15430.1.
X69109 Genomic DNA. No translation available.
PIRiA64924.
RefSeqiWP_001174940.1. NZ_LN832404.1.
YP_025307.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP37340.
SMRiP37340. Positions 1-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260264. 200 interactions.
STRINGi511145.b1663.

Chemistry

BindingDBiP37340.
ChEMBLiCHEMBL1681613.

Protein family/group databases

TCDBi2.A.66.1.3. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Proteomic databases

PaxDbiP37340.
PRIDEiP37340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48136; AAT48136; b1663.
BAA15430; BAA15430; BAA15430.
GeneIDi945883.
KEGGiecj:JW1655.
eco:b1663.
PATRICi32118632. VBIEscCol129921_1736.

Organism-specific databases

EchoBASEiEB2300.
EcoGeneiEG12400. mdtK.

Phylogenomic databases

eggNOGiENOG4107QI1. Bacteria.
COG0534. LUCA.
HOGENOMiHOG000038538.
InParanoidiP37340.
KOiK03327.
OMAiSIVFMLP.
PhylomeDBiP37340.

Enzyme and pathway databases

BioCyciEcoCyc:YDHE-MONOMER.
ECOL316407:JW1655-MONOMER.

Miscellaneous databases

PROiP37340.

Family and domain databases

HAMAPiMF_00400. MdtK. 1 hit.
InterProiIPR002528. MATE_fam.
IPR022913. Multidrug-R_MdtK.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
PIRSFiPIRSF006603. DinF. 1 hit.
TIGRFAMsiTIGR00797. matE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDTK_ECOLI
AccessioniPrimary (citable) accession number: P37340
Secondary accession number(s): P77276, P77765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.