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Protein

L-2-hydroxyglutarate oxidase LhgO

Gene

lhgO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate.UniRule annotation1 Publication

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.UniRule annotation1 Publication

Cofactori

FADUniRule annotation1 Publication

Kineticsi

  1. KM=95 µM for L-2-hydroxyglutarate1 Publication
  1. Vmax=113 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:EG12387-MONOMER.
ECOL316407:JW2635-MONOMER.
MetaCyc:EG12387-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-2-hydroxyglutarate oxidase LhgOUniRule annotation (EC:1.1.3.15UniRule annotation)
Gene namesi
Name:lhgOUniRule annotation
Synonyms:ygaF
Ordered Locus Names:b2660, JW2635
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12387. lhgO.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001692951 – 422L-2-hydroxyglutarate oxidase LhgOAdd BLAST422

Proteomic databases

PaxDbiP37339.
PRIDEiP37339.

Expressioni

Inductioni

Expression is induced by RpoS during carbon starvation and at stationary phase. Is also regulated by cAMP-CRP. Repressed by CsiR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263106. 8 interactors.
DIPiDIP-12096N.
IntActiP37339. 4 interactors.
MINTiMINT-1301841.
STRINGi511145.b2660.

Structurei

3D structure databases

ProteinModelPortaliP37339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L2HGDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D5U. Bacteria.
COG0579. LUCA.
HOGENOMiHOG000245180.
InParanoidiP37339.
KOiK15736.
OMAiGVHFTRM.
PhylomeDBiP37339.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_00990. L_hydroxyglutarate_oxidase. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR030862. L_hydroxyglutarate_Oxase.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.

Sequencei

Sequence statusi: Complete.

P37339-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDFVIIGGG IIGMSTAMQL IDVYPDARIA LLEKESAPAC HQTGHNSGVI
60 70 80 90 100
HAGVYYTPGS LKAQFCLAGN RATKAFCDQN GIRYDNCGKM LVATSDLEME
110 120 130 140 150
RMRALWERTA ANGIEREWLN ADELREREPN ITGLGGIFVP SSGIVSYRDV
160 170 180 190 200
TAAMAKIFQS RGGEIIYNAE VSGLNEHKNG VVIRTRQGGE YEASTLISCS
210 220 230 240 250
GLMADRLVKM LGLEPGFIIC PFRGEYFRLA PEHNQIVNHL IYPIPDPAMP
260 270 280 290 300
FLGVHLTRMI DGSVTVGPNA VLAFKREGYR KRDFSFSDTL EILGSSGIRR
310 320 330 340 350
VLQNHLRSGL GEMKNSLCKS GYLRLVQKYC PRLSLSDLQP WPAGVRAQAV
360 370 380 390 400
SPDGKLIDDF LFVTTPRTIH TCNAPSPAAT SAIPIGAHIV SKVQTLLASQ
410 420
SNPGRTLRAA RSVDALHAAF NQ
Length:422
Mass (Da):46,082
Last modified:December 4, 2007 - v3
Checksum:i8E13E890B0A7365C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75707.2.
AP009048 Genomic DNA. Translation: BAA16521.1.
M88334 Genomic DNA. No translation available.
PIRiE65045.
RefSeqiNP_417146.2. NC_000913.3.
WP_000271909.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75707; AAC75707; b2660.
BAA16521; BAA16521; BAA16521.
GeneIDi948069.
KEGGiecj:JW2635.
eco:b2660.
PATRICi32120710. VBIEscCol129921_2752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75707.2.
AP009048 Genomic DNA. Translation: BAA16521.1.
M88334 Genomic DNA. No translation available.
PIRiE65045.
RefSeqiNP_417146.2. NC_000913.3.
WP_000271909.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263106. 8 interactors.
DIPiDIP-12096N.
IntActiP37339. 4 interactors.
MINTiMINT-1301841.
STRINGi511145.b2660.

Proteomic databases

PaxDbiP37339.
PRIDEiP37339.

Protocols and materials databases

DNASUi948069.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75707; AAC75707; b2660.
BAA16521; BAA16521; BAA16521.
GeneIDi948069.
KEGGiecj:JW2635.
eco:b2660.
PATRICi32120710. VBIEscCol129921_2752.

Organism-specific databases

EchoBASEiEB2288.
EcoGeneiEG12387. lhgO.

Phylogenomic databases

eggNOGiENOG4105D5U. Bacteria.
COG0579. LUCA.
HOGENOMiHOG000245180.
InParanoidiP37339.
KOiK15736.
OMAiGVHFTRM.
PhylomeDBiP37339.

Enzyme and pathway databases

BioCyciEcoCyc:EG12387-MONOMER.
ECOL316407:JW2635-MONOMER.
MetaCyc:EG12387-MONOMER.

Miscellaneous databases

PROiP37339.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_00990. L_hydroxyglutarate_oxidase. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR030862. L_hydroxyglutarate_Oxase.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLHGO_ECOLI
AccessioniPrimary (citable) accession number: P37339
Secondary accession number(s): P76622, P77020
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.