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P37339 (LHGO_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-2-hydroxyglutarate oxidase LhgO

EC=1.1.3.15
Gene names
Name:lhgO
Synonyms:ygaF
Ordered Locus Names:b2660, JW2635
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate. Ref.7

Catalytic activity

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2. Ref.7

Cofactor

FAD. Ref.7

Induction

Expression is induced by RpoS during carbon starvation and at stationary phase. Is also regulated by cAMP-CRP. Repressed by CsiR. Ref.6

Sequence similarities

Belongs to the L2HGDH family.

Biophysicochemical properties

Kinetic parameters:

KM=95 µM for L-2-hydroxyglutarate Ref.7

Vmax=113 nmol/min/mg enzyme

Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP0A6F51EBI-555990,EBI-543750

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422L-2-hydroxyglutarate oxidase LhgO HAMAP-Rule MF_00990
PRO_0000169295

Sequences

Sequence LengthMass (Da)Tools
P37339 [UniParc].

Last modified December 4, 2007. Version 3.
Checksum: 8E13E890B0A7365C

FASTA42246,082
        10         20         30         40         50         60 
MYDFVIIGGG IIGMSTAMQL IDVYPDARIA LLEKESAPAC HQTGHNSGVI HAGVYYTPGS 

        70         80         90        100        110        120 
LKAQFCLAGN RATKAFCDQN GIRYDNCGKM LVATSDLEME RMRALWERTA ANGIEREWLN 

       130        140        150        160        170        180 
ADELREREPN ITGLGGIFVP SSGIVSYRDV TAAMAKIFQS RGGEIIYNAE VSGLNEHKNG 

       190        200        210        220        230        240 
VVIRTRQGGE YEASTLISCS GLMADRLVKM LGLEPGFIIC PFRGEYFRLA PEHNQIVNHL 

       250        260        270        280        290        300 
IYPIPDPAMP FLGVHLTRMI DGSVTVGPNA VLAFKREGYR KRDFSFSDTL EILGSSGIRR 

       310        320        330        340        350        360 
VLQNHLRSGL GEMKNSLCKS GYLRLVQKYC PRLSLSDLQP WPAGVRAQAV SPDGKLIDDF 

       370        380        390        400        410        420 
LFVTTPRTIH TCNAPSPAAT SAIPIGAHIV SKVQTLLASQ SNPGRTLRAA RSVDALHAAF 


NQ 

« Hide

References

« Hide 'large scale' references
[1]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Molecular organization of the Escherichia coli gab cluster: nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene."
Niegemann E., Schulz A., Bartsch K.
Arch. Microbiol. 160:454-460(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 219-422.
Strain: K12 / JM103 / ATCC 39403 / DSM 2829 / NCIMB 12044.
[5]"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
Borodovsky M., Rudd K.E., Koonin E.V.
Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[6]"Multiple stress signal integration in the regulation of the complex sigma S-dependent csiD-ygaF-gabDTP operon in Escherichia coli."
Metzner M., Germer J., Hengge R.
Mol. Microbiol. 51:799-811(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[7]"Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase."
Kalliri E., Mulrooney S.B., Hausinger R.P.
J. Bacteriol. 190:3793-3798(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC75707.2.
AP009048 Genomic DNA. Translation: BAA16521.1.
M88334 Genomic DNA. No translation available.
PIRE65045.
RefSeqNP_417146.2. NC_000913.3.
YP_490875.1. NC_007779.1.

3D structure databases

ProteinModelPortalP37339.
SMRP37339. Positions 2-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-12096N.
IntActP37339. 4 interactions.
MINTMINT-1301841.
STRING511145.b2660.

Proteomic databases

PaxDbP37339.

Protocols and materials databases

DNASU948069.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75707; AAC75707; b2660.
BAA16521; BAA16521; BAA16521.
GeneID12934112.
948069.
KEGGecj:Y75_p2603.
eco:b2660.
PATRIC32120710. VBIEscCol129921_2752.

Organism-specific databases

EchoBASEEB2288.
EcoGeneEG12387. lhgO.

Phylogenomic databases

eggNOGCOG0579.
HOGENOMHOG000245180.
KOK15736.
OMAGVHFTRM.
OrthoDBEOG6X9MHV.
PhylomeDBP37339.
ProtClustDBPRK11728.

Enzyme and pathway databases

BioCycEcoCyc:EG12387-MONOMER.
ECOL316407:JW2635-MONOMER.
MetaCyc:EG12387-MONOMER.

Gene expression databases

GenevestigatorP37339.

Family and domain databases

HAMAPMF_00990. L_hydroxyglutarate_oxidase.
InterProIPR006076. FAD-dep_OxRdtase.
[Graphical view]
PfamPF01266. DAO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP37339.

Entry information

Entry nameLHGO_ECOLI
AccessionPrimary (citable) accession number: P37339
Secondary accession number(s): P76622, P77020
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene