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Protein

L-2-hydroxyglutarate oxidase LhgO

Gene

lhgO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate.UniRule annotation1 Publication

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.UniRule annotation1 Publication

Cofactori

FADUniRule annotation1 Publication

Kineticsi

  1. KM=95 µM for L-2-hydroxyglutarate1 Publication
  1. Vmax=113 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:EG12387-MONOMER
MetaCyc:EG12387-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
L-2-hydroxyglutarate oxidase LhgOUniRule annotation (EC:1.1.3.15UniRule annotation)
Gene namesi
Name:lhgOUniRule annotation
Synonyms:ygaF
Ordered Locus Names:b2660, JW2635
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12387 lhgO

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • plasma membrane Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001692951 – 422L-2-hydroxyglutarate oxidase LhgOAdd BLAST422

Proteomic databases

PaxDbiP37339
PRIDEiP37339

Expressioni

Inductioni

Expression is induced by RpoS during carbon starvation and at stationary phase. Is also regulated by cAMP-CRP. Repressed by CsiR.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ppiCP0A9L53EBI-555990,EBI-555953

Protein-protein interaction databases

BioGridi4263106, 11 interactors
DIPiDIP-12096N
IntActiP37339, 4 interactors
STRINGi316385.ECDH10B_2827

Structurei

3D structure databases

ProteinModelPortaliP37339
SMRiP37339
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L2HGDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D5U Bacteria
COG0579 LUCA
HOGENOMiHOG000245180
InParanoidiP37339
KOiK15736
OMAiGVHFTRM
PhylomeDBiP37339

Family and domain databases

Gene3Di3.50.50.60, 3 hits
HAMAPiMF_00990 L_hydroxyglutarate_oxidase, 1 hit
InterProiView protein in InterPro
IPR006076 FAD-dep_OxRdtase
IPR036188 FAD/NAD-bd_sf
IPR030862 L_hydroxyglutarate_Oxase
PfamiView protein in Pfam
PF01266 DAO, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit

Sequencei

Sequence statusi: Complete.

P37339-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDFVIIGGG IIGMSTAMQL IDVYPDARIA LLEKESAPAC HQTGHNSGVI
60 70 80 90 100
HAGVYYTPGS LKAQFCLAGN RATKAFCDQN GIRYDNCGKM LVATSDLEME
110 120 130 140 150
RMRALWERTA ANGIEREWLN ADELREREPN ITGLGGIFVP SSGIVSYRDV
160 170 180 190 200
TAAMAKIFQS RGGEIIYNAE VSGLNEHKNG VVIRTRQGGE YEASTLISCS
210 220 230 240 250
GLMADRLVKM LGLEPGFIIC PFRGEYFRLA PEHNQIVNHL IYPIPDPAMP
260 270 280 290 300
FLGVHLTRMI DGSVTVGPNA VLAFKREGYR KRDFSFSDTL EILGSSGIRR
310 320 330 340 350
VLQNHLRSGL GEMKNSLCKS GYLRLVQKYC PRLSLSDLQP WPAGVRAQAV
360 370 380 390 400
SPDGKLIDDF LFVTTPRTIH TCNAPSPAAT SAIPIGAHIV SKVQTLLASQ
410 420
SNPGRTLRAA RSVDALHAAF NQ
Length:422
Mass (Da):46,082
Last modified:December 4, 2007 - v3
Checksum:i8E13E890B0A7365C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75707.2
AP009048 Genomic DNA Translation: BAA16521.1
M88334 Genomic DNA No translation available.
PIRiE65045
RefSeqiNP_417146.2, NC_000913.3
WP_000271909.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75707; AAC75707; b2660
BAA16521; BAA16521; BAA16521
GeneIDi948069
KEGGiecj:JW2635
eco:b2660
PATRICifig|1411691.4.peg.4081

Similar proteinsi

Entry informationi

Entry nameiLHGO_ECOLI
AccessioniPrimary (citable) accession number: P37339
Secondary accession number(s): P76622, P77020
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 4, 2007
Last modified: April 25, 2018
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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