Reviewed, UniProtKB/Swiss-Prot P37337 (BPHG_BURXL)

Names and origin

Protein names

Recommended name:
    Biphenyl dioxygenase system ferredoxin--NAD(+) reductase component
    EC=1.18.1.3

Gene names

Name:	bphG
Ordered Locus Names:	Bxeno_C1127
ORF Names:	Bxe_C1193

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the electron transfer component of biphenyl dioxygenase, transfers electrons from ferredoxin (bphF) to NADH.
Catalytic activity	Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.
Cofactor	FAD.
Pathway	Xenobiotic degradation; biphenyl degradation.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (bphA and bphE), a ferredoxin (bphF) and a ferredoxin reductase (bphG).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NAD+ reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	Biphenyl dioxygenase system ferredoxin--NAD(+) reductase component		PRO_0000167653
Regions
	Nucleotide binding	4 – 35	32	FAD Potential
	Nucleotide binding	145 – 173	29	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P37337-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 8A52BB01688667A9	FASTA	408	42,954
	10 20 30 40 50 60 MIDTIAIIGA GLAGSTAARA LRAQGYEGRI HLLGDESHQA YDRTTLSKTV LAGEQPEPPA 70 80 90 100 110 120 ILDSAWYASA HVDVQLGRRV SCLDLANRQI QFESGAPLAY DRLLLATGAR ARRMAIRGGD 130 140 150 160 170 180 LAGIHTLRDL ADSQALRQAL QPGQSLVIVG GGLIGCEVAT TARKLSVHVT ILEAGDELLV 190 200 210 220 230 240 RVLGHRTGAW CRAELERMGV RVERNAQAAR FEGQGQVRAV ICADGRRVPA DVVLVSIGAE 250 260 270 280 290 300 PADELARAAG IACARGVLVD ATGATSCPEV FAAGDVAAWP LRQGGQRSLE TYLNSQMEAE 310 320 330 340 350 360 IAASAMLSQP VPAPQVPTSW TEIAGHRIQM IGDAEGPGEI VVRGDAQSGQ PIVLLRLLDG 370 380 390 400 CVEAATAINA TREFSVATRL VGTRVSVSAE QLQDVGSNLR DLLKAKPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400." Erickson B.D., Mondello F.J. J. Bacteriol. 174:2903-2912(1992) [PubMed: 1569021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erickson B.D., Mondello F.J. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86348 Genomic DNA. Translation: AAB63429.1. CP000272 Genomic DNA. Translation: ABE37055.1.
PIR	F41858.
RefSeq	YP_556405.1.
3D structure databases
HSSP	HSSP built from PDB template 1D7Y based on UniProtKB Q52437.
ModBase	Search...
Genome annotation databases
GeneID	4010705.
GenomeReviews	Gene locus Bxeno_C1127 in contig CP000272_GR.
KEGG	bxe:Bxe_C1193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P37337.
OMA	CEVATTA.
Enzyme and pathway databases
BioCyc	BXEN266265:BXE_C1193-MON.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

BPHG_BURXL

Accession

Primary (citable) accession number: P37337
Secondary accession number(s): Q13FT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)