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Protein

Biphenyl dioxygenase subunit beta

Gene

bphE

Organism
Paraburkholderia xenovorans (strain LB400)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The beta subunit may be responsible for the substrate specificity of the enzyme.

Catalytic activityi

Biphenyl + NADH + O2 = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+.

Pathwayi: biphenyl degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Biphenyl dioxygenase subunit alpha (bphA), Biphenyl dioxygenase subunit beta (bphE)
  2. Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (bphB)
  3. Biphenyl-2,3-diol 1,2-dioxygenase (bphC)
  4. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD)
This subpathway is part of the pathway biphenyl degradation, which is itself part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl, the pathway biphenyl degradation and in Xenobiotic degradation.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processAromatic hydrocarbons catabolism
LigandNAD

Enzyme and pathway databases

BioCyciBXEN266265:G1G66-8628-MONOMER
BRENDAi1.14.12.18 7691
UniPathwayiUPA00155; UER00250

Names & Taxonomyi

Protein namesi
Recommended name:
Biphenyl dioxygenase subunit beta (EC:1.14.12.18)
Alternative name(s):
Biphenyl 2,3-dioxygenase
Gene namesi
Name:bphE
Ordered Locus Names:Bxeno_C1130
ORF Names:Bxe_C1196
OrganismiParaburkholderia xenovorans (strain LB400)
Taxonomic identifieri266265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001817 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000850682 – 188Biphenyl dioxygenase subunit betaAdd BLAST187

Interactioni

Subunit structurei

Heterohexamer consisting of three BphA subunits and three BphE subunits. A ferredoxin (BphF) and a ferredoxin reductase (BphG) must be present to obtain activity.

Protein-protein interaction databases

STRINGi266265.Bxe_C1196

Structurei

Secondary structure

1188
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 12Combined sources4
Helixi21 – 39Combined sources19
Helixi43 – 47Combined sources5
Beta strandi50 – 59Combined sources10
Helixi66 – 71Combined sources6
Beta strandi80 – 84Combined sources5
Helixi86 – 97Combined sources12
Helixi102 – 104Combined sources3
Beta strandi109 – 121Combined sources13
Beta strandi127 – 140Combined sources14
Turni141 – 143Combined sources3
Beta strandi144 – 158Combined sources15
Beta strandi160 – 175Combined sources16
Beta strandi177 – 182Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XR8X-ray2.49B/D/F/H/J/L/N/P/R/T/V/X1-188[»]
2XRXX-ray2.42B/D/F/H/J/L/N/P/R/T/V/X1-188[»]
2XSHX-ray2.29B/D/F/H/J/L1-188[»]
2XSOX-ray2.20B/D/F/H/J/L/N/P/R/T/V/X1-188[»]
2YFIX-ray2.15B/D/F/H/J/L1-188[»]
2YFJX-ray2.15B/D/F/H/J/L1-188[»]
2YFLX-ray2.60B/D/F/H/J/L1-188[»]
5AEUX-ray2.49B/D/F/H1-188[»]
5AEWX-ray1.88B/D/F/H/J/L/N/P/R/T/V/X1-188[»]
ProteinModelPortaliP37334
SMRiP37334
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37334

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106DX9 Bacteria
ENOG410YRH3 LUCA
HOGENOMiHOG000106036
KOiK15750
OMAiLQAHQFL
OrthoDBiPOG091H0C0Q

Family and domain databases

CDDicd00667 ring_hydroxylating_dioxygenase, 1 hit
InterProiView protein in InterPro
IPR032710 NTF2-like_dom_sf
IPR000391 Rng_hydr_dOase-bsu
PANTHERiPTHR41534 PTHR41534, 1 hit
PfamiView protein in Pfam
PF00866 Ring_hydroxyl_B, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNPSPHFFK TFEWPSKAAG LELQNEIEQF YYREAQLLDH RAYEAWFALL
60 70 80 90 100
DKDIHYFMPL RTNRMIREGE LEYSGDQDLA HFDETHETMY GRIRKVTSDV
110 120 130 140 150
GWAENPPSRT RHLVSNVIVK ETATPDTFEV NSAFILYRNR LERQVDIFAG
160 170 180
ERRDVLRRAD NNLGFSIAKR TILLDASTLL SNNLSMFF
Length:188
Mass (Da):22,085
Last modified:January 23, 2007 - v3
Checksum:i36DBE14E22FEA67B
GO

Sequence cautioni

The sequence ABE37058 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86348 Genomic DNA Translation: AAB63426.1
CP000272 Genomic DNA Translation: ABE37058.1 Different initiation.
RefSeqiWP_040123616.1, NZ_CP008761.1

Genome annotation databases

EnsemblBacteriaiABE37058; ABE37058; Bxe_C1196
GeneIDi4010708
KEGGibxb:DR64_8609
bxe:Bxe_C1196
PATRICifig|266265.5.peg.8946

Similar proteinsi

Entry informationi

Entry nameiBPHE_PARXL
AccessioniPrimary (citable) accession number: P37334
Secondary accession number(s): Q13FT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 110 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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