Reviewed, UniProtKB/Swiss-Prot P37334 (BPHE_BURXL)

Names and origin

Protein names

Recommended name:
    Biphenyl dioxygenase subunit beta
    EC=1.14.12.18
Alternative name(s):
    Biphenyl 2,3-dioxygenase

Gene names

Name:	bphE
Ordered Locus Names:	Bxeno_C1130
ORF Names:	Bxe_C1196

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Biphenyl + NADH + O2 = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoic acid and benzoic acid from biphenyl: step 1/4.
Subunit structure	Heterohexamer consisting of three BphA subunits and three BphE subunits. A ferredoxin (BphF) and a ferredoxin reductase (BphG) must be present to obtain activity.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	biphenyl 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 188	187	Biphenyl dioxygenase subunit beta		PRO_0000085068

Sequences

	Sequence		Length	Mass (Da)
	P37334-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 36DBE14E22FEA67B	FASTA	188	22,085
	10 20 30 40 50 60 MTNPSPHFFK TFEWPSKAAG LELQNEIEQF YYREAQLLDH RAYEAWFALL DKDIHYFMPL 70 80 90 100 110 120 RTNRMIREGE LEYSGDQDLA HFDETHETMY GRIRKVTSDV GWAENPPSRT RHLVSNVIVK 130 140 150 160 170 180 ETATPDTFEV NSAFILYRNR LERQVDIFAG ERRDVLRRAD NNLGFSIAKR TILLDASTLL SNNLSMFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400." Erickson B.D., Mondello F.J. J. Bacteriol. 174:2903-2912(1992) [PubMed: 1569021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400." Haddock J.D., Gibson D.T. J. Bacteriol. 177:5834-5839(1995) [PubMed: 7592331] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12, CHARACTERIZATION.
[4]	Erratum Haddock J.D., Gibson D.T. J. Bacteriol. 178:258-258(1996)

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86348 Genomic DNA. Translation: AAB63426.1. CP000272 Genomic DNA. Translation: ABE37058.1. Different initiation.
RefSeq	YP_556408.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4010708.
GenomeReviews	Gene locus Bxeno_C1130 in contig CP000272_GR.
KEGG	bxe:Bxe_C1196.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P37334.
OMA	P37334. DIFAGER.
Enzyme and pathway databases
BioCyc	BXEN266265:BXE_C1196-MON.
Family and domain databases
InterPro	IPR000391. Rng_hydr_dOase-bsu. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BPHE_BURXL

Accession

Primary (citable) accession number: P37334
Secondary accession number(s): Q13FT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	May 5, 2009
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)