P37334 (BPHE_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biphenyl dioxygenase subunit beta
EC=1.14.12.18

Alternative name(s):
Biphenyl 2,3-dioxygenase

Gene names

Name:	bphE
Ordered Locus Names:	Bxeno_C1130
ORF Names:	Bxe_C1196

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Biphenyl + NADH + O2 = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl: step 1/4.
Subunit structure	Heterohexamer consisting of three BphA subunits and three BphE subunits. A ferredoxin (BphF) and a ferredoxin reductase (BphG) must be present to obtain activity.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.
Sequence caution	The sequence ABE37058.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	biphenyl 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 188

187

Biphenyl dioxygenase subunit beta

PRO_0000085068

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

188

Helix Strand Turn

Details...

Helix

22 – 39

18

Helix

43 – 47

5

Beta strand

50 – 59

10

Beta strand

80 – 84

5

Helix

86 – 89

4

Helix

93 – 97

5

Beta strand

109 – 121

13

Beta strand

127 – 140

14

Turn

141 – 143

3

Beta strand

144 – 148

5

Beta strand

152 – 157

6

Beta strand

166 – 172

7

Beta strand

177 – 182

6

Sequences

	Sequence		Length	Mass (Da)
	P37334 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 36DBE14E22FEA67B	FASTA	188	22,085
	10 20 30 40 50 60 MTNPSPHFFK TFEWPSKAAG LELQNEIEQF YYREAQLLDH RAYEAWFALL DKDIHYFMPL 70 80 90 100 110 120 RTNRMIREGE LEYSGDQDLA HFDETHETMY GRIRKVTSDV GWAENPPSRT RHLVSNVIVK 130 140 150 160 170 180 ETATPDTFEV NSAFILYRNR LERQVDIFAG ERRDVLRRAD NNLGFSIAKR TILLDASTLL SNNLSMFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400." Erickson B.D., Mondello F.J. J. Bacteriol. 174:2903-2912(1992) [PubMed: 1569021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.
[3]	"Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400." Haddock J.D., Gibson D.T. J. Bacteriol. 177:5834-5839(1995) [PubMed: 7592331] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12, CHARACTERIZATION.
[4]	Erratum Haddock J.D., Gibson D.T. J. Bacteriol. 178:258-258(1996)
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M86348 Genomic DNA. Translation: AAB63426.1.
CP000272 Genomic DNA. Translation: ABE37058.1. Different initiation.

RefSeq

YP_556408.1. NC_007953.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XR8	X-ray	2.49	B/D/F/H/J/L/N/P/R/T/V/X	1-188	[»]
2XRX	X-ray	2.42	B/D/F/H/J/L/N/P/R/T/V/X	1-188	[»]
2XSH	X-ray	2.29	B/D/F/H/J/L	1-188	[»]
2XSO	X-ray	2.20	B/D/F/H/J/L/N/P/R/T/V/X	1-188	[»]
2YFI	X-ray	2.15	B/D/F/H/J/L	1-188	[»]
2YFJ	X-ray	2.15	B/D/F/H/J/L	1-188	[»]

ProteinModelPortal

P37334.

SMR

P37334. Positions 10-188.

ModBase

Search...

Protein-protein interaction databases

STRING

P37334.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

4010708.

GenomeReviews

Gene locus Bxeno_C1130 in contig CP000272_GR.

KEGG

bxe:Bxe_C1196.

PATRIC

19343377. VBIBurXen52548_8946.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG582498.

OMA

DIFAGER.

ProtClustDB

CLSK922463.

Enzyme and pathway databases

BioCyc

BXEN266265:BXE_C1196-MONOMER.

Family and domain databases

InterPro

IPR000391. Rng_hydr_dOase-bsu.
[Graphical view]

KO

K08689.

Pfam

PF00866. Ring_hydroxyl_B. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BPHE_BURXL

Accession

Primary (citable) accession number: P37334
Secondary accession number(s): Q13FT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program