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P37332 (BPHF_BURXL) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biphenyl dioxygenase system ferredoxin subunit
Gene names
Name:bphF
Ordered Locus Names:Bxeno_C1128
ORF Names:Bxe_C1194
OrganismBurkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]
Taxonomic identifier266265 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length109 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein seems to be a 2Fe-2S ferredoxin.

Subunit structure

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (BphA and BphE), a ferredoxin (BphF) and a ferredoxin reductase (BphG).

Sequence similarities

Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin component family.

Contains 1 Rieske domain.

Biophysicochemical properties

Redox potential:

E0 is -157 mV.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 109109Biphenyl dioxygenase system ferredoxin subunit
PRO_0000201686

Regions

Domain4 – 10097Rieske

Sites

Metal binding431Iron-sulfur (2Fe-2S)
Metal binding451Iron-sulfur (2Fe-2S); via pros nitrogen
Metal binding631Iron-sulfur (2Fe-2S)
Metal binding661Iron-sulfur (2Fe-2S); via pros nitrogen

Secondary structure

1 ......................... 109
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P37332 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 39AE9C8B84232FA4

FASTA10911,955
        10         20         30         40         50         60 
MKFTRVCDRR DVPEGEALKV ESGGTSVAIF NVDGELFATQ DRCTHGDWSL SDGGYLEGDV 

        70         80         90        100 
VECSLHMGKF CVRTGKVKSP PPCEALKIFP IRIEDNDVLV DFEAGYLAP

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400."
Erickson B.D., Mondello F.J.
J. Bacteriol. 174:2903-2912(1992) [PubMed: 1569021] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Erickson B.D., Mondello F.J.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility."
Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. expand/collapse author list , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M.
Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LB400.
[4]"Characterization of BphF, a Rieske-type ferredoxin with a low reduction potential."
Couture M.M.-J., Colbert C.L., Babini E., Rosell F.I., Mauk A.G., Bolin J.T., Eltis L.D.
Biochemistry 40:84-92(2001) [PubMed: 11141059] [Abstract]
Cited for: CHARACTERIZATION.
[5]"A cluster exposed: structure of the Rieske ferredoxin from biphenyl dioxygenase and the redox properties of Rieske Fe-S proteins."
Colbert C.L., Couture M.M.-J., Eltis L.D., Bolin J.T.
Structure 8:1267-1278(2000) [PubMed: 11188691] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M86348 Genomic DNA. Translation: AAB63428.1.
CP000272 Genomic DNA. Translation: ABE37056.1.
PIRE41858.
RefSeqYP_556406.1. NC_007953.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FQTX-ray1.60A/B1-109[»]
ProteinModelPortalP37332.
SMRP37332. Positions 1-109.
ModBaseSearch...

Protein-protein interaction databases

STRINGP37332.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4010706.
GenomeReviewsGene locus Bxeno_C1128 in contig CP000272_GR.
KEGGbxe:Bxe_C1194.
PATRIC19343373. VBIBurXen52548_8944.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG668715.
OMAEDECTHA.
ProtClustDBCLSK922464.

Enzyme and pathway databases

BioCycBXEN266265:BXE_C1194-MONOMER.

Family and domain databases

InterProIPR017941. Rieske_2Fe-2S.
[Graphical view]
Gene3DG3DSA:2.102.10.10. Rieske_reg. 1 hit.
KOK08689.
PfamPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMSSF50022. Rieske_dom. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBPHF_BURXL
AccessionPrimary (citable) accession number: P37332
Secondary accession number(s): Q13FT3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1996
Last modified: January 25, 2012
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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