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Protein

Biphenyl dioxygenase system ferredoxin subunit

Gene

bphF

Organism
Burkholderia xenovorans (strain LB400)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein seems to be a 2Fe-2S ferredoxin.

Redox potential

E0 is -157 mV.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Iron-sulfur (2Fe-2S)
Metal bindingi45 – 451Iron-sulfur (2Fe-2S); via pros nitrogen
Metal bindingi63 – 631Iron-sulfur (2Fe-2S)
Metal bindingi66 – 661Iron-sulfur (2Fe-2S); via pros nitrogen

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Aromatic hydrocarbons catabolism, Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBXEN266265:GJII-8846-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Biphenyl dioxygenase system ferredoxin subunit
Gene namesi
Name:bphF
Ordered Locus Names:Bxeno_C1128
ORF Names:Bxe_C1194
OrganismiBurkholderia xenovorans (strain LB400)
Taxonomic identifieri266265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001817 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 109109Biphenyl dioxygenase system ferredoxin subunitPRO_0000201686Add
BLAST

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (BphA and BphE), a ferredoxin (BphF) and a ferredoxin reductase (BphG).

Protein-protein interaction databases

STRINGi266265.Bxe_C1194.

Structurei

Secondary structure

1
109
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Helixi9 – 113Combined sources
Beta strandi17 – 226Combined sources
Beta strandi25 – 328Combined sources
Beta strandi35 – 428Combined sources
Beta strandi49 – 513Combined sources
Beta strandi60 – 623Combined sources
Turni64 – 663Combined sources
Beta strandi69 – 713Combined sources
Turni72 – 743Combined sources
Beta strandi77 – 815Combined sources
Beta strandi91 – 944Combined sources
Beta strandi97 – 1004Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FQTX-ray1.60A/B1-109[»]
ProteinModelPortaliP37332.
SMRiP37332. Positions 1-109.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37332.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 10097RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VQD. Bacteria.
COG2146. LUCA.
HOGENOMiHOG000128541.
KOiK18087.
OMAiVECSLHM.
OrthoDBiEOG6QVRCJ.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTRVCDRR DVPEGEALKV ESGGTSVAIF NVDGELFATQ DRCTHGDWSL
60 70 80 90 100
SDGGYLEGDV VECSLHMGKF CVRTGKVKSP PPCEALKIFP IRIEDNDVLV

DFEAGYLAP
Length:109
Mass (Da):11,955
Last modified:February 1, 1996 - v2
Checksum:i39AE9C8B84232FA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86348 Genomic DNA. Translation: AAB63428.1.
CP000272 Genomic DNA. Translation: ABE37056.1.
PIRiE41858.

Genome annotation databases

EnsemblBacteriaiABE37056; ABE37056; Bxe_C1194.
KEGGibxb:DR64_8611.
bxe:Bxe_C1194.
PATRICi19343373. VBIBurXen52548_8944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86348 Genomic DNA. Translation: AAB63428.1.
CP000272 Genomic DNA. Translation: ABE37056.1.
PIRiE41858.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FQTX-ray1.60A/B1-109[»]
ProteinModelPortaliP37332.
SMRiP37332. Positions 1-109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266265.Bxe_C1194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE37056; ABE37056; Bxe_C1194.
KEGGibxb:DR64_8611.
bxe:Bxe_C1194.
PATRICi19343373. VBIBurXen52548_8944.

Phylogenomic databases

eggNOGiENOG4105VQD. Bacteria.
COG2146. LUCA.
HOGENOMiHOG000128541.
KOiK18087.
OMAiVECSLHM.
OrthoDBiEOG6QVRCJ.

Enzyme and pathway databases

BioCyciBXEN266265:GJII-8846-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37332.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400."
    Erickson B.D., Mondello F.J.
    J. Bacteriol. 174:2903-2912(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Erickson B.D., Mondello F.J.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LB400.
  4. "Characterization of BphF, a Rieske-type ferredoxin with a low reduction potential."
    Couture M.M.-J., Colbert C.L., Babini E., Rosell F.I., Mauk A.G., Bolin J.T., Eltis L.D.
    Biochemistry 40:84-92(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "A cluster exposed: structure of the Rieske ferredoxin from biphenyl dioxygenase and the redox properties of Rieske Fe-S proteins."
    Colbert C.L., Couture M.M.-J., Eltis L.D., Bolin J.T.
    Structure 8:1267-1278(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiBPHF_BURXL
AccessioniPrimary (citable) accession number: P37332
Secondary accession number(s): Q13FT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.