Reviewed, UniProtKB/Swiss-Prot P37332 (BPHF_BURXL)

Names and origin

Protein names

Recommended name:
    Biphenyl dioxygenase system ferredoxin subunit

Gene names

Name:	bphF
Ordered Locus Names:	Bxeno_C1128
ORF Names:	Bxe_C1194

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein seems to be a 2Fe-2S ferredoxin.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (bphA and bphE), a ferredoxin (bphF) and a ferredoxin reductase (bphG).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin component family. Contains 1 Rieske domain.
biophysicochemical properties	Redox potential: E0 is -157 mV.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Electron transport Transport
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 109

109

Biphenyl dioxygenase system ferredoxin subunit

PRO_0000201686

Regions

Domain

4 – 100

97

Rieske

Sites

Metal binding

43

1

Iron-sulfur (2Fe-2S)

Metal binding

45

1

Iron-sulfur (2Fe-2S); via pros nitrogen

Metal binding

63

1

Iron-sulfur (2Fe-2S)

Metal binding

66

1

Iron-sulfur (2Fe-2S); via pros nitrogen

Secondary structure

1

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109

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

9 – 11

3

Beta strand

17 – 22

6

Beta strand

25 – 32

8

Beta strand

35 – 42

8

Beta strand

49 – 51

3

Beta strand

60 – 62

3

Turn

64 – 66

3

Beta strand

69 – 71

3

Turn

72 – 74

3

Beta strand

77 – 81

5

Beta strand

91 – 94

4

Beta strand

97 – 100

4

Sequences

	Sequence		Length	Mass (Da)
	P37332-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 39AE9C8B84232FA4	FASTA	109	11,955
	10 20 30 40 50 60 MKFTRVCDRR DVPEGEALKV ESGGTSVAIF NVDGELFATQ DRCTHGDWSL SDGGYLEGDV 70 80 90 100 VECSLHMGKF CVRTGKVKSP PPCEALKIFP IRIEDNDVLV DFEAGYLAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400." Erickson B.D., Mondello F.J. J. Bacteriol. 174:2903-2912(1992) [PubMed: 1569021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erickson B.D., Mondello F.J. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Characterization of BphF, a Rieske-type ferredoxin with a low reduction potential." Couture M.M.-J., Colbert C.L., Babini E., Rosell F.I., Mauk A.G., Bolin J.T., Eltis L.D. Biochemistry 40:84-92(2001) [PubMed: 11141059] [Abstract] Cited for: CHARACTERIZATION.
[5]	"A cluster exposed: structure of the Rieske ferredoxin from biphenyl dioxygenase and the redox properties of Rieske Fe-S proteins." Colbert C.L., Couture M.M.-J., Eltis L.D., Bolin J.T. Structure 8:1267-1278(2000) [PubMed: 11188691] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

M86348 Genomic DNA. Translation: AAB63428.1.
CP000272 Genomic DNA. Translation: ABE37056.1.

PIR

E41858.

RefSeq

YP_556406.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FQT	X-ray	1.60	A/B	1-109	[»]

ModBase

Search...

Genome annotation databases

GeneID

4010706.

GenomeReviews

Gene locus Bxeno_C1128 in contig CP000272_GR.

KEGG

bxe:Bxe_C1194.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P37332.

OMA

P37332. ECWAHGS.

Enzyme and pathway databases

BioCyc

BXEN266265:BXE_C1194-MON.

Family and domain databases

InterPro

IPR017941. Rieske_2Fe-2S.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

Pfam

PF00355. Rieske. 1 hit.
[Graphical view]

PROSITE

PS51296. RIESKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BPHF_BURXL

Accession

Primary (citable) accession number: P37332
Secondary accession number(s): Q13FT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)