Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Molybdate-binding periplasmic protein

Gene

modA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of molybdenum into the cell. Binds molybdate with high specificity and affinity.

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:MODA-MONOMER.
ECOL316407:JW0746-MONOMER.
MetaCyc:MODA-MONOMER.
RETL1328306-WGS:GSTH-4183-MONOMER.

Protein family/group databases

TCDBi3.A.1.8.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Molybdate-binding periplasmic protein
Gene namesi
Name:modA
Ordered Locus Names:b0763, JW0746
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12427. modA.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 257233Molybdate-binding periplasmic proteinPRO_0000031827Add
BLAST

Proteomic databases

PaxDbiP37329.
PRIDEiP37329.

2D gel databases

SWISS-2DPAGEP37329.

Interactioni

Protein-protein interaction databases

BioGridi4262087. 14 interactions.
IntActiP37329. 1 interaction.
STRINGi511145.b0763.

Structurei

Secondary structure

1
257
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 347Combined sources
Helixi35 – 373Combined sources
Helixi38 – 5215Combined sources
Beta strandi55 – 617Combined sources
Helixi63 – 7210Combined sources
Beta strandi77 – 804Combined sources
Helixi84 – 929Combined sources
Helixi98 – 1003Combined sources
Beta strandi102 – 1076Combined sources
Beta strandi109 – 1168Combined sources
Helixi130 – 1345Combined sources
Beta strandi139 – 1424Combined sources
Turni144 – 1463Combined sources
Helixi148 – 15912Combined sources
Helixi163 – 1664Combined sources
Helixi167 – 1693Combined sources
Beta strandi170 – 1756Combined sources
Helixi176 – 1849Combined sources
Beta strandi187 – 1948Combined sources
Helixi195 – 2006Combined sources
Beta strandi202 – 2098Combined sources
Helixi212 – 2143Combined sources
Beta strandi218 – 2258Combined sources
Helixi231 – 24010Combined sources
Helixi243 – 2519Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AMFX-ray1.75A25-257[»]
1WODX-ray1.75A25-257[»]
3AXFX-ray2.00A/B/C25-257[»]
3R26X-ray1.70A25-257[»]
ProteinModelPortaliP37329.
SMRiP37329. Positions 27-257.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37329.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108S6J. Bacteria.
COG0725. LUCA.
HOGENOMiHOG000280537.
InParanoidiP37329.
KOiK02020.
OMAiLTHENLW.
OrthoDBiEOG680X4N.
PhylomeDBiP37329.

Family and domain databases

InterProiIPR005950. Mo_ABC_perip-bd.
[Graphical view]
PIRSFiPIRSF004846. ModA. 1 hit.
TIGRFAMsiTIGR01256. modA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37329-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKWLNLFA GAALSFAVAG NALADEGKIT VFAAASLTNA MQDIATQFKK
60 70 80 90 100
EKGVDVVSSF ASSSTLARQI EAGAPADLFI SADQKWMDYA VDKKAIDTAT
110 120 130 140 150
RQTLLGNSLV VVAPKASVQK DFTIDSKTNW TSLLNGGRLA VGDPEHVPAG
160 170 180 190 200
IYAKEALQKL GAWDTLSPKL APAEDVRGAL ALVERNEAPL GIVYGSDAVA
210 220 230 240 250
SKGVKVVATF PEDSHKKVEY PVAVVEGHNN ATVKAFYDYL KGPQAAEIFK

RYGFTIK
Length:257
Mass (Da):27,364
Last modified:October 1, 1994 - v1
Checksum:i847DB740EE0564E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34009 Genomic DNA. Translation: AAB00835.1.
U27192 Genomic DNA. Translation: AAB60171.1.
U07867 Genomic DNA. Translation: AAB06893.1.
U00096 Genomic DNA. Translation: AAC73850.1.
AP009048 Genomic DNA. Translation: BAA35427.1.
PIRiC64812.
RefSeqiNP_415284.1. NC_000913.3.
WP_000101984.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73850; AAC73850; b0763.
BAA35427; BAA35427; BAA35427.
GeneIDi945364.
KEGGiecj:JW0746.
eco:b0763.
PATRICi32116727. VBIEscCol129921_0789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34009 Genomic DNA. Translation: AAB00835.1.
U27192 Genomic DNA. Translation: AAB60171.1.
U07867 Genomic DNA. Translation: AAB06893.1.
U00096 Genomic DNA. Translation: AAC73850.1.
AP009048 Genomic DNA. Translation: BAA35427.1.
PIRiC64812.
RefSeqiNP_415284.1. NC_000913.3.
WP_000101984.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AMFX-ray1.75A25-257[»]
1WODX-ray1.75A25-257[»]
3AXFX-ray2.00A/B/C25-257[»]
3R26X-ray1.70A25-257[»]
ProteinModelPortaliP37329.
SMRiP37329. Positions 27-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262087. 14 interactions.
IntActiP37329. 1 interaction.
STRINGi511145.b0763.

Protein family/group databases

TCDBi3.A.1.8.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP37329.

Proteomic databases

PaxDbiP37329.
PRIDEiP37329.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73850; AAC73850; b0763.
BAA35427; BAA35427; BAA35427.
GeneIDi945364.
KEGGiecj:JW0746.
eco:b0763.
PATRICi32116727. VBIEscCol129921_0789.

Organism-specific databases

EchoBASEiEB2325.
EcoGeneiEG12427. modA.

Phylogenomic databases

eggNOGiENOG4108S6J. Bacteria.
COG0725. LUCA.
HOGENOMiHOG000280537.
InParanoidiP37329.
KOiK02020.
OMAiLTHENLW.
OrthoDBiEOG680X4N.
PhylomeDBiP37329.

Enzyme and pathway databases

BioCyciEcoCyc:MODA-MONOMER.
ECOL316407:JW0746-MONOMER.
MetaCyc:MODA-MONOMER.
RETL1328306-WGS:GSTH-4183-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37329.
PROiP37329.

Family and domain databases

InterProiIPR005950. Mo_ABC_perip-bd.
[Graphical view]
PIRSFiPIRSF004846. ModA. 1 hit.
TIGRFAMsiTIGR01256. modA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availability."
    Rech S., Deppenmeier U., Gunsalus R.P.
    J. Bacteriol. 177:1023-1029(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene."
    Walkenhorst H.M., Hemschemeier S.K., Eichenlaub R.
    Microbiol. Res. 150:347-361(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MC1000 / ATCC 39531.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Properties of the periplasmic ModA molybdate-binding protein of Escherichia coli."
    Rech S., Wolin C., Gunsalus R.P.
    J. Biol. Chem. 271:2557-2562(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-31, CHARACTERIZATION.
  7. "Crystal structure of the molybdate binding protein ModA."
    Hu Y., Rech S., Gunsalus R.P., Rees D.C.
    Nat. Struct. Biol. 4:703-707(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 25-257.

Entry informationi

Entry nameiMODA_ECOLI
AccessioniPrimary (citable) accession number: P37329
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 20, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.