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Protein

Putative prophage CPS-53 integrase

Gene

intS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei360 – 3601O-(3'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
  • prophage integrase activity Source: GO_Central
  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • DNA recombination Source: UniProtKB-KW
  • provirus excision Source: EcoCyc
  • viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Enzyme and pathway databases

BioCyciEcoCyc:G7218-MONOMER.
ECOL316407:JW2345-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative prophage CPS-53 integrase
Gene namesi
Name:intS
Synonyms:intC, yfdB
Ordered Locus Names:b2349, JW2345
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12413. intS.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Putative prophage CPS-53 integrasePRO_0000197518Add
BLAST

Proteomic databases

PaxDbiP37326.
PRIDEiP37326.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259384. 12 interactions.
DIPiDIP-10041N.
IntActiP37326. 12 interactions.
STRINGi511145.b2349.

Structurei

Secondary structure

1
385
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi93 – 10412Combined sources
Turni105 – 1073Combined sources
Helixi110 – 12819Combined sources
Helixi138 – 14912Combined sources
Turni150 – 1523Combined sources
Helixi154 – 17320Combined sources
Helixi182 – 19110Combined sources
Helixi192 – 1943Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KJ8NMR-A87-196[»]
ProteinModelPortaliP37326.
SMRiP37326. Positions 2-197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37326.

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

eggNOGiENOG4105DFP. Bacteria.
COG0582. LUCA.
HOGENOMiHOG000263105.
InParanoidiP37326.
OMAiQIPAFNQ.
OrthoDBiEOG6D8B85.
PhylomeDBiP37326.

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR025166. DUF4102.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR023109. Integrase_recombinase_N.
[Graphical view]
PfamiPF13356. DUF4102. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P37326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTVKQIEAA KPKEKPYRLL DGNGLYLYVP VSGKKVWQLR YKIDGKEKIL
60 70 80 90 100
TVGKYPLMTL QEARDKAWTA RKDISVGIDP VKAKKASSNN NSFSAIYKEW
110 120 130 140 150
YEHKKQVWSV GYATELAKMF DDDILPIIGG LEIQDIEPMQ LLEVIRRFED
160 170 180 190 200
RGAMERANKA RRRCGEVFRY AIVTGRAKYN PAPDLADAMK GYRKKNFPFL
210 220 230 240 250
PADQIPAFNK ALATFSGSIV SLIATKVLRY TALRTKELRS MLWKNVDFEN
260 270 280 290 300
RIITIDASVM KGRKIHVVPM SDQVVELLTT LSSITKPVSE FVFAGRNDKK
310 320 330 340 350
KPICENAVLL VIKQIGYEGL ESGHGFRHEF STIMNEHEWP ADAIEVQLAH
360 370 380
ANGGSVRGIY NHAQYLDKRR EMMQWWADWL DEKVE
Length:385
Mass (Da):44,061
Last modified:October 1, 1994 - v1
Checksum:iC3D9B1C59A06DBBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11296 Unassigned DNA. Translation: AAA19609.1.
U00096 Genomic DNA. Translation: AAC75408.1.
AP009048 Genomic DNA. Translation: BAA16208.1.
PIRiI84552.
RefSeqiNP_416850.1. NC_000913.3.
WP_000958671.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75408; AAC75408; b2349.
BAA16208; BAA16208; BAA16208.
GeneIDi946821.
KEGGiecj:JW2345.
eco:b2349.
PATRICi32120073. VBIEscCol129921_2445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11296 Unassigned DNA. Translation: AAA19609.1.
U00096 Genomic DNA. Translation: AAC75408.1.
AP009048 Genomic DNA. Translation: BAA16208.1.
PIRiI84552.
RefSeqiNP_416850.1. NC_000913.3.
WP_000958671.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KJ8NMR-A87-196[»]
ProteinModelPortaliP37326.
SMRiP37326. Positions 2-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259384. 12 interactions.
DIPiDIP-10041N.
IntActiP37326. 12 interactions.
STRINGi511145.b2349.

Proteomic databases

PaxDbiP37326.
PRIDEiP37326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75408; AAC75408; b2349.
BAA16208; BAA16208; BAA16208.
GeneIDi946821.
KEGGiecj:JW2345.
eco:b2349.
PATRICi32120073. VBIEscCol129921_2445.

Organism-specific databases

EchoBASEiEB2312.
EcoGeneiEG12413. intS.

Phylogenomic databases

eggNOGiENOG4105DFP. Bacteria.
COG0582. LUCA.
HOGENOMiHOG000263105.
InParanoidiP37326.
OMAiQIPAFNQ.
OrthoDBiEOG6D8B85.
PhylomeDBiP37326.

Enzyme and pathway databases

BioCyciEcoCyc:G7218-MONOMER.
ECOL316407:JW2345-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37326.
PROiP37326.

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR025166. DUF4102.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR023109. Integrase_recombinase_N.
[Graphical view]
PfamiPF13356. DUF4102. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Baumann S.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiINTS_ECOLI
AccessioniPrimary (citable) accession number: P37326
Secondary accession number(s): P76942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 20, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.