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P37323 (BLA4_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase SHV-4

EC=3.5.2.6
Alternative name(s):
Ceftazidimase 5
Short name=CAZ-5
Gene names
Name:bla
Synonyms:shv4
Encoded onPlasmid pUD21
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

SHV enzymes hydrolyze broad spectrum cephalosporins notably cefotaxime and ceftazidime. SHV-4 causes particularly high levels of resistance to aztreonam and ceftazidime.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termDirect protein sequencing
Plasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Beta-lactamase SHV-4
PRO_0000195440

Regions

Region209 – 2113Substrate binding By similarity

Sites

Active site451Acyl-ester intermediate By similarity
Active site1431Proton acceptor By similarity

Amino acid modifications

Disulfide bond52 ↔ 98 By similarity

Sequences

Sequence LengthMass (Da)Tools
P37323 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: E575CF38B1ADE304

FASTA26528,860
        10         20         30         40         50         60 
SPQPLEQIKL SESQLSGRVG MIEMDLASGR TLTAWRADER FPMMSTFKVV LCGAVLARVD 

        70         80         90        100        110        120 
AGDEQLERKI HYRQQDLVDY SPVSEKHLAD GMTVGELCAA AITMSDNSAA NLLLATVGGP 

       130        140        150        160        170        180 
AGLTAFLRQI GDNVTRLDRW ETELNEALPG DARDTTTPAS MAATLRKLLT SQRLSARSQL 

       190        200        210        220        230        240 
QLLQWMVDDR VAGPLIRSVL PAGWFIADKT GASKRGARGI VALLGPNNKA ERIVVIYLRD 

       250        260 
TPASMAERNQ QIAGIGAALI EHWQR 

« Hide

References

[1]"Structural features related to hydrolytic activity against ceftazidime of plasmid-mediated SHV-type CAZ-5 beta-lactamase."
Peduzzi J., Barthelemy M., Tiwari K., Mattioni D., Labia R.
Antimicrob. Agents Chemother. 33:2160-2163(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Strain: 210-2.

Cross-references

Sequence databases

PIRA60448.

3D structure databases

ProteinModelPortalP37323.
SMRP37323. Positions 1-265.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLA4_KLEPN
AccessionPrimary (citable) accession number: P37323
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 16, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families