Reviewed, UniProtKB/Swiss-Prot P37323 (BLA4_KLEPN)

Names and origin

Protein names

Recommended name:
    Beta-lactamase SHV-4
    EC=3.5.2.6
Alternative name(s):
    Ceftazidimase 5
      Short name=CAZ-5

Gene names

Name:	bla
Synonyms:	shv4

Encoded on

Plasmid pUD21

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	SHV enzymes hydrolyze broad spectrum cephalosporins notably cefotaxime and ceftazidime. SHV-4 causes particularly high levels of resistance to aztreonam and ceftazidime.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Beta-lactamase SHV-4		PRO_0000195440
Regions
	Region	209 – 211	3	Substrate binding By similarity
Sites
	Active site	45	1	Acyl-ester intermediate By similarity
	Active site	143	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	52 ↔ 98		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37323-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: E575CF38B1ADE304	FASTA	265	28,860
	10 20 30 40 50 60 SPQPLEQIKL SESQLSGRVG MIEMDLASGR TLTAWRADER FPMMSTFKVV LCGAVLARVD 70 80 90 100 110 120 AGDEQLERKI HYRQQDLVDY SPVSEKHLAD GMTVGELCAA AITMSDNSAA NLLLATVGGP 130 140 150 160 170 180 AGLTAFLRQI GDNVTRLDRW ETELNEALPG DARDTTTPAS MAATLRKLLT SQRLSARSQL 190 200 210 220 230 240 QLLQWMVDDR VAGPLIRSVL PAGWFIADKT GASKRGARGI VALLGPNNKA ERIVVIYLRD 250 260 TPASMAERNQ QIAGIGAALI EHWQR

References

[1]

"Structural features related to hydrolytic activity against ceftazidime of plasmid-mediated SHV-type CAZ-5 beta-lactamase." Peduzzi J., Barthelemy M., Tiwari K., Mattioni D., Labia R. Antimicrob. Agents Chemother. 33:2160-2163(1989) [PubMed: 2694955] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: 210-2.

Cross-references

Sequence databases
PIR	A60448.
3D structure databases
SMR	P37323. Positions 1-265.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 758.
Family and domain databases
InterPro	IPR012338. B-lactamase-typ_transpept_fold. IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA4_KLEPN

Accession

Primary (citable) accession number: P37323

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 24, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)