Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein PAM1

Gene

PAM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Not known. It is a suppressor of protein phosphatase 2A depletion.

GO - Biological processi

  • pseudohyphal growth Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29823-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PAM1
Gene namesi
Name:PAM1
Ordered Locus Names:YDR251W
ORF Names:YD8419.18, YD9320A.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR251W.
SGDiS000002659. PAM1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830Protein PAM1PRO_0000058220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei659 – 6591PhosphoserineCombined sources
Modified residuei732 – 7321PhosphoserineCombined sources
Modified residuei767 – 7671PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP37304.
PeptideAtlasiP37304.
PRIDEiP37304.

PTM databases

iPTMnetiP37304.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PKH3Q033063EBI-12870,EBI-37683

Protein-protein interaction databases

BioGridi32301. 35 interactions.
DIPiDIP-5213N.
IntActiP37304. 11 interactions.
MINTiMINT-485906.

Structurei

3D structure databases

ProteinModelPortaliP37304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili379 – 40022Add
BLAST
Coiled coili481 – 51434Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi515 – 5228Poly-Gln
Compositional biasi810 – 83021Arg/Lys-rich (highly basic)Add
BLAST

Sequence similaritiesi

Belongs to the PAM1/SVL3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000068916.
HOGENOMiHOG000115436.
InParanoidiP37304.
OrthoDBiEOG7JQBWT.

Sequencei

Sequence statusi: Complete.

P37304-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSALRVLVC GDHPNLILYT SRFQHAKNIE FYLVNNSKNA NYEVSSLFYG
60 70 80 90 100
TERFQIQNHF QSLLDLVDLN NENGGLVFDL IIMSASSLQE IPQVLRDIKP
110 120 130 140 150
MMNKTTKILF ESSGFIYLEP FIKASVDLSL SNIFSIFTDY DIRRLDNGSY
160 170 180 190 200
KQFTTANAKS FSVSIGQTTS VHENSYSSDI IPILNTFQKL FQKLFPRDVV
210 220 230 240 250
TLYDHSPSAF LAKEWELALP QICFDPLLII LEEKNPSTLD DHVLAKPLIS
260 270 280 290 300
GLLGESLLII KKMGIAMNNP NFQNEQTILK HWKNKCEDLP DGPALLYNFI
310 320 330 340 350
HKASSLNIDL LLLQPILLAD DFGVKTPYLE CLFTMMTQYQ LLNKGDSEWF
360 370 380 390 400
IRKDENTALT RVDDLQNSIA LKDGKIMQLQ NSESTLKNEI KELQSQVLSL
410 420 430 440 450
KQEVSSSKAN NGQELEILKK KVQMGDNSLF DRPNSNTNGI SPSDNIVDVD
460 470 480 490 500
LNYERSDQGN NSSGNDSRRQ SFFNSTSDTT LSRDETSLKE RELEVRMKEL
510 520 530 540 550
ELQERELELQ RKALQQQQQY QQRPPKQVYS GPSGTPTSGN NNNKSYNPNR
560 570 580 590 600
KSSYSQPQHV AMMTSRGLHG PSAASSSPVI SANNFVDPVS SGTPYSSNSS
610 620 630 640 650
RFSQQIPSQQ YMHTVKPTSR KNRSSVMPNI GYVPGLTNNE YGRKFNGNGM
660 670 680 690 700
NGTQSRLNSL SNQSTFRSQQ GPPITQQKSF QNNGGSMRTN RIPSANYNIS
710 720 730 740 750
NQQSGFVNSI SSPNLSNLEN RNTVQNSRNA DSAPCVNQLN SDSPPQLQSL
760 770 780 790 800
SQNGTSKVPQ INITQPSPIQ TNFATSDNPA AVIKLGTPSE DTVSAAATAN
810 820 830
NISTMGDESR KEDVKEKKKK KFSFFGKRKK
Length:830
Mass (Da):92,872
Last modified:December 21, 2004 - v2
Checksum:i23DC105C4AB45FB9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti256 – 2561S → T in CAA51833 (PubMed:8106383).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73454 Genomic DNA. Translation: CAA51833.1.
Z49701 Genomic DNA. Translation: CAA89737.1.
Z70202 Genomic DNA. Translation: CAA94090.1.
Z68329 Genomic DNA. Translation: CAA92708.1.
BK006938 Genomic DNA. Translation: DAA12091.1.
PIRiS54547.
RefSeqiNP_010537.3. NM_001180559.3.

Genome annotation databases

EnsemblFungiiYDR251W; YDR251W; YDR251W.
GeneIDi851838.
KEGGisce:YDR251W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73454 Genomic DNA. Translation: CAA51833.1.
Z49701 Genomic DNA. Translation: CAA89737.1.
Z70202 Genomic DNA. Translation: CAA94090.1.
Z68329 Genomic DNA. Translation: CAA92708.1.
BK006938 Genomic DNA. Translation: DAA12091.1.
PIRiS54547.
RefSeqiNP_010537.3. NM_001180559.3.

3D structure databases

ProteinModelPortaliP37304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32301. 35 interactions.
DIPiDIP-5213N.
IntActiP37304. 11 interactions.
MINTiMINT-485906.

PTM databases

iPTMnetiP37304.

Proteomic databases

MaxQBiP37304.
PeptideAtlasiP37304.
PRIDEiP37304.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR251W; YDR251W; YDR251W.
GeneIDi851838.
KEGGisce:YDR251W.

Organism-specific databases

EuPathDBiFungiDB:YDR251W.
SGDiS000002659. PAM1.

Phylogenomic databases

GeneTreeiENSGT00530000068916.
HOGENOMiHOG000115436.
InParanoidiP37304.
OrthoDBiEOG7JQBWT.

Enzyme and pathway databases

BioCyciYEAST:G3O-29823-MONOMER.

Miscellaneous databases

PROiP37304.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Overexpression of yeast PAM1 gene permits survival without protein phosphatase 2A and induces a filamentous phenotype."
    Hu G.-Z., Ronne H.
    J. Biol. Chem. 269:3429-3435(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 208353 / W303-1A.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-659, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-659 AND SER-767, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-659 AND SER-732, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPAM1_YEAST
AccessioniPrimary (citable) accession number: P37304
Secondary accession number(s): D6VSN1, Q03789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 21, 2004
Last modified: June 8, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.