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Protein

Low specificity L-threonine aldolase

Gene

GLY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of L-allo-threonine and L-threonine to glycine and acetaldehyde.

Catalytic activityi

L-threonine = glycine + acetaldehyde.1 Publication
L-allo-threonine = glycine + acetaldehyde.1 Publication

Cofactori

Pathwayi: glycine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glycine from L-allo-threonine.
Proteins known to be involved in this subpathway in this organism are:
  1. Low specificity L-threonine aldolase (GLY1)
This subpathway is part of the pathway glycine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-allo-threonine, the pathway glycine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-threonine degradation via aldolase pathway

This protein is involved in step 1 of the subpathway that synthesizes acetaldehyde and glycine from L-threonine.
Proteins known to be involved in this subpathway in this organism are:
  1. Low specificity L-threonine aldolase (GLY1)
This subpathway is part of the pathway L-threonine degradation via aldolase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetaldehyde and glycine from L-threonine, the pathway L-threonine degradation via aldolase pathway and in Amino-acid degradation.

GO - Molecular functioni

  • L-allo-threonine aldolase activity Source: SGD
  • threonine aldolase activity Source: SGD

GO - Biological processi

  • glycine biosynthetic process Source: SGD
  • threonine catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12832.
YEAST:YEL046C-MONOMER.
BRENDAi4.1.2.48. 984.
UniPathwayiUPA00044; UER00429.
UPA00288; UER00427.

Names & Taxonomyi

Protein namesi
Recommended name:
Low specificity L-threonine aldolase (EC:4.1.2.48)
Short name:
Low specificity L-TA
Short name:
TA
Gene namesi
Name:GLY1
Ordered Locus Names:YEL046C
ORF Names:SYGP-ORF34
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL046C.
SGDiS000000772. GLY1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Low specificity L-threonine aldolasePRO_0000121572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei213 – 2131N6-(pyridoxal phosphate)lysineBy similarity
Cross-linki228 – 228Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources2 Publications
Modified residuei367 – 3671PhosphoserineCombined sources
Modified residuei369 – 3691PhosphoserineCombined sources
Modified residuei370 – 3701PhosphothreonineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP37303.
PRIDEiP37303.

PTM databases

iPTMnetiP37303.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi36684. 31 interactions.
DIPiDIP-4287N.
IntActiP37303. 6 interactions.
MINTiMINT-560402.

Structurei

3D structure databases

ProteinModelPortaliP37303.
SMRiP37303. Positions 16-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the threonine aldolase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014681.
HOGENOMiHOG000219824.
InParanoidiP37303.
KOiK01620.
OMAiLPVQTNM.
OrthoDBiEOG7GXPMP.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR023603. Threonine_aldolase.
[Graphical view]
PfamiPF01212. Beta_elim_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF017617. Thr_aldolase. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

P37303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEFELPPKY ITAANDLRSD TFTTPTAEMM EAALEASIGD AVYGEDVDTV
60 70 80 90 100
RLEQTVARMA GKEAGLFCVS GTLSNQIAIR THLMQPPYSI LCDYRAHVYT
110 120 130 140 150
HEAAGLAILS QAMVVPVVPS NGDYLTLEDI KSHYVPDDGD IHGAPTRLIS
160 170 180 190 200
LENTLHGIVY PLEELVRIKA WCMENGLKLH CDGARIWNAA AQSGVPLKQY
210 220 230 240 250
GEIFDSISIC LSKSMGAPIG SVLVGNLKFV KKATHFRKQQ GGGIRQSGMM
260 270 280 290 300
ARMALVNINN DWKSQLLYSH SLAHELAEYC EAKGIPLESP ADTNFVFINL
310 320 330 340 350
KAARMDPDVL VKKGLKYNVK LMGGRVSFHY QVTRDTLEKV KLAISEAFDY
360 370 380
AKEHPFDCNG PTQIYRSEST EVDVDGNAIR EIKTYKY
Length:387
Mass (Da):42,815
Last modified:October 5, 2010 - v2
Checksum:i46139EB459442852
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2531M → I in AAA72430 (PubMed:8132653).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28739 Genomic DNA. Translation: AAA72430.1.
U18779 Genomic DNA. Translation: AAB64996.1.
BK006939 Genomic DNA. Translation: DAA07608.1.
PIRiS30831.
RefSeqiNP_010868.1. NM_001178861.1.

Genome annotation databases

EnsemblFungiiYEL046C; YEL046C; YEL046C.
GeneIDi856665.
KEGGisce:YEL046C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28739 Genomic DNA. Translation: AAA72430.1.
U18779 Genomic DNA. Translation: AAB64996.1.
BK006939 Genomic DNA. Translation: DAA07608.1.
PIRiS30831.
RefSeqiNP_010868.1. NM_001178861.1.

3D structure databases

ProteinModelPortaliP37303.
SMRiP37303. Positions 16-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36684. 31 interactions.
DIPiDIP-4287N.
IntActiP37303. 6 interactions.
MINTiMINT-560402.

PTM databases

iPTMnetiP37303.

Proteomic databases

MaxQBiP37303.
PRIDEiP37303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL046C; YEL046C; YEL046C.
GeneIDi856665.
KEGGisce:YEL046C.

Organism-specific databases

EuPathDBiFungiDB:YEL046C.
SGDiS000000772. GLY1.

Phylogenomic databases

GeneTreeiENSGT00390000014681.
HOGENOMiHOG000219824.
InParanoidiP37303.
KOiK01620.
OMAiLPVQTNM.
OrthoDBiEOG7GXPMP.

Enzyme and pathway databases

UniPathwayiUPA00044; UER00429.
UPA00288; UER00427.
BioCyciMetaCyc:MONOMER-12832.
YEAST:YEL046C-MONOMER.
BRENDAi4.1.2.48. 984.

Miscellaneous databases

PROiP37303.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR023603. Threonine_aldolase.
[Graphical view]
PfamiPF01212. Beta_elim_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF017617. Thr_aldolase. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and molecular characterization of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine."
    McNeil J.B., McIntosh E.M., Taylor B.V., Zhang F.-R., Tang S., Bognar A.L.
    J. Biol. Chem. 269:9155-9165(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 201238 / W303-1B.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The GLY1 gene of Saccharomyces cerevisiae encodes a low-specific L-threonine aldolase that catalyzes cleavage of L-allo-threonine and L-threonine to glycine -- expression of the gene in Escherichia coli and purification and characterization of the enzyme."
    Liu J.-Q., Nagata S., Dairi T., Misono H., Shimizu S., Yamada H.
    Eur. J. Biochem. 245:289-293(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, CHARACTERIZATION.
  5. "Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis."
    Monschau N., Stahmann K.-P., Sahm H., McNeil J.B., Bognar A.L.
    FEMS Microbiol. Lett. 150:55-60(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-228.
    Strain: SUB592.
  8. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
    Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
    Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-228.
  9. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-367; SER-369 AND THR-370, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-228, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLY1_YEAST
AccessioniPrimary (citable) accession number: P37303
Secondary accession number(s): D3DLK4, P32615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 5, 2010
Last modified: July 6, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 106000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.