P37302 (APE3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminopeptidase Y
EC=3.4.11.15

Gene names

Name:	APE3
Ordered Locus Names:	YBR286W
ORF Names:	YBR2024

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Preferentially, release of N-terminal lysine.
Cofactor	Binds 1 zinc ion per subunit.
Subunit structure	Monomer.
Subcellular location	Vacuole. Note: Lysosome-like vacuoles.
Miscellaneous	Present with 4740 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase M28 family. M28A subfamily.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Ligand	Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
PTM	Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW vacuolar protein catabolic process Inferred from direct assay Ref.5. Source: SGD
Cellular_component	fungal-type vacuole Inferred from direct assay Ref.5. Source: SGD
Molecular_function	aminopeptidase activity Inferred from direct assay Ref.5. Source: SGD metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SEH1	P53011	1	EBI-2649,EBI-16940

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 56	35	Potential		PRO_0000026847
	Chain	57 – 537	481	Aminopeptidase Y		PRO_0000026848
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	150	1	N-linked (GlcNAc...) Potential
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	371	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential
	Glycosylation	480	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	14 – 15	2	YA → LR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P37302 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: B34B7819194CF7F3	FASTA	537	60,137
	10 20 30 40 50 60 MHFSLKQLAV AAFYATNLGS AYVIPQFFQE AFQQEEPIEN YLPQLNDDDS SAVAANIPKP 70 80 90 100 110 120 HIPYFMKPHV ESEKLQDKIK VDDLNATAWD LYRLANYSTP DYGHPTRVIG SKGHNKTMEY 130 140 150 160 170 180 ILNVFDDMQD YYDVSLQEFE ALSGKIISFN LSDAETGKSF ANTTAFALSP PVDGFVGKLV 190 200 210 220 230 240 EIPNLGCEEK DYASVVPPRH NEKQIALIER GKCPFGDKSN LAGKFGFTAV VIYDNEPKSK 250 260 270 280 290 300 EGLHGTLGEP TKHTVATVGV PYKVGKKLIA NIALNIDYSL YFAMDSYVEF IKTQNIIADT 310 320 330 340 350 360 KHGDPDNIVA LGAHSDSVEE GPGINDDGSG TISLLNVAKQ LTHFKINNKV RFAWWAAEEE 370 380 390 400 410 420 GLLGSNFYAY NLTKEENSKI RVFMDYDMMA SPNYEYEIYD ANNKENPKGS EELKNLYVDY 430 440 450 460 470 480 YKAHHLNYTL VPFDGRSDYV GFINNGIPAG GIATGAEKNN VNNGKVLDRC YHQLCDDVSN 490 500 510 520 530 LSWDAFITNT KLIAHSVATY ADSFEGFPKR ETQKHKEVDI LNAQQPQFKY RADFLII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the aminopeptidase Y gene of Saccharomyces cerevisiae. Sequence analysis and gene disruption of a new aminopeptidase." Nishizawa M., Yasuhara T., Nakai T., Fujiki Y., Ohashi A. J. Biol. Chem. 269:13651-13655(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 57-70. Strain: ATCC 24657 / D273-10B.
[2]	"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Holmstroem K., Brandt T., Kallesoe T. Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Aminopeptidase Y, a new aminopeptidase from Saccharomyces cerevisiae. Purification, properties, localization, and processing by protease B." Yasuhara T., Nakai T., Ohashi A. J. Biol. Chem. 269:13644-13650(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 57-70, CHARACTERIZATION. Strain: D273-1B.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L31635 Unassigned DNA. Translation: AAA19559.1. X76053 Genomic DNA. Translation: CAA53649.1. Z36155 Genomic DNA. Translation: CAA85251.1. BK006936 Genomic DNA. Translation: DAA07401.1.
PIR	A54134.
RefSeq	NP_009845.2. NM_001178634.1.
3D structure databases
ProteinModelPortal	P37302.
SMR	P37302. Positions 295-501.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3975N.
IntAct	P37302. 2 interactions.
MINT	MINT-542381.
STRING	4932.YBR286W.
Protein family/group databases
MEROPS	M28.001.
Proteomic databases
PaxDb	P37302.
PeptideAtlas	P37302.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR286W; YBR286W; YBR286W.
GeneID	852589.
KEGG	sce:YBR286W.
Organism-specific databases
CYGD	YBR286w.
SGD	S000000490. APE3.
Phylogenomic databases
eggNOG	COG2234.
HOGENOM	HOG000051000.
KO	K01264.
OMA	CYHQLCD.
OrthoDB	EOG4TB7KV.
Enzyme and pathway databases
BioCyc	YEAST:YBR286W-MONOMER.
Gene expression databases
Genevestigator	P37302.
GermOnline	YBR286W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007484. Peptidase_M28. IPR003137. Protease-assoc_domain. [Graphical view]
Pfam	PF02225. PA. 1 hit. PF04389. Peptidase_M28. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971748.

Entry information

Entry name

APE3_YEAST

Accession

Primary (citable) accession number: P37302
Secondary accession number(s): D6VQT1, P38154

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 29, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program