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Protein

Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial

Gene

SDH4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in system II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH3 and SDH4 form the membrane dimer that anchors the catalytic dimer formed by SDH1 and SDH2 to the matrix surface of the mitochondrial inner membrane. Electrons originating from the catalytic dimer enter the membrane dimer for ubiquinone reduction.

Miscellaneous

Present with 7920 molecules/cell in log phase SD medium.1 Publication

Pathwayi: tricarboxylic acid cycle

This protein is involved in the pathway tricarboxylic acid cycle, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Iron (heme axial ligand)Curated1
Binding sitei120Ubiquinone; shared with IP/SDHBBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular respiration Source: SGD
  • mitochondrial electron transport, succinate to ubiquinone Source: SGD
  • tricarboxylic acid cycle Source: SGD

Keywordsi

Biological processElectron transport, Transport, Tricarboxylic acid cycle
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YDR178W-MONOMER
YEAST:YDR178W-MONOMER
UniPathwayiUPA00223

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
Short name:
CybS
Alternative name(s):
Succinate-ubiquinone reductase membrane anchor subunit
Gene namesi
Name:SDH4
Synonyms:ACN18
Ordered Locus Names:YDR178W
ORF Names:YD9395.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR178W
SGDiS000002585 SDH4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 66Mitochondrial matrixSequence analysisAdd BLAST35
Transmembranei67 – 88HelicalSequence analysisAdd BLAST22
Topological domaini89 – 98Mitochondrial intermembraneSequence analysis10
Transmembranei99 – 118HelicalSequence analysisAdd BLAST20
Topological domaini119 – 127Mitochondrial matrixSequence analysis9
Transmembranei128 – 148HelicalSequence analysisAdd BLAST21
Topological domaini149 – 181Mitochondrial intermembraneSequence analysisAdd BLAST33

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi100F → V: Impairs respiratory growth and reduces quinone reductase activity. 1 Publication1
Mutagenesisi102S → A: Impairs respiratory growth and reduces quinone reductase activity. 1 Publication1
Mutagenesisi109C → A: Decreases SDH cytochrome b content. 1 Publication1
Mutagenesisi120Y → C: Abolishes quinone reductase activity. Little effect on complex assembly. 1 Publication1
Mutagenesisi130H → L: Reduces SDH FAD content. Probably impairs complex assembly. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 31Mitochondrion1 PublicationAdd BLAST31
ChainiPRO_000000649632 – 181Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrialAdd BLAST150

Keywords - PTMi

Quinone

Proteomic databases

MaxQBiP37298
PaxDbiP37298
PRIDEiP37298

PTM databases

iPTMnetiP37298

Interactioni

Subunit structurei

Forms part of complex II containing four subunits: a flavoprotein (FP), an iron-sulfur protein (IP) and a cytochrome b composed of a large and a small subunit.

Protein-protein interaction databases

BioGridi32231, 90 interactors
DIPiDIP-5704N
IntActiP37298, 5 interactors
MINTiP37298
STRINGi4932.YDR178W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ORZmodel-D32-181[»]
1PB4model-D32-181[»]
ProteinModelPortaliP37298
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CybS family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000009201
HOGENOMiHOG000204209
InParanoidiP37298
KOiK00237
OMAiAHGSYHW
OrthoDBiEOG092C5DUX

Family and domain databases

CDDicd03496 SQR_TypeC_CybS, 1 hit
Gene3Di1.20.1300.10, 1 hit
InterProiView protein in InterPro
IPR007992 CybS
IPR034804 SQR/QFR_C/D
PANTHERiPTHR13337 PTHR13337, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37298-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLPRSMKFM TGRRIFHTAT VRAFQSTAKK SLTIPFLPVL PQKPGGVRGT
60 70 80 90 100
PNDAYVPPPE NKLEGSYHWY MEKIFALSVV PLATTAMLTT GPLSTAADSF
110 120 130 140 150
FSVMLLGYCY MEFNSCITDY ISERVYGVWH KYAMYMLGLG SAVSLFGIYK
160 170 180
LETENDGVVG LVKSLWDSSE KDNSQKIEAK K
Length:181
Mass (Da):20,249
Last modified:October 1, 1994 - v1
Checksum:i80D76A32E7E6DA70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26333 Unassigned RNA Translation: AAA19637.1
Z46727 Genomic DNA Translation: CAA86683.1
AY557671 Genomic DNA Translation: AAS55997.1
BK006938 Genomic DNA Translation: DAA12020.1
PIRiA54380
RefSeqiNP_010463.1, NM_001180485.1

Genome annotation databases

EnsemblFungiiYDR178W; YDR178W; YDR178W
GeneIDi851758
KEGGisce:YDR178W

Similar proteinsi

Entry informationi

Entry nameiDHSD_YEAST
AccessioniPrimary (citable) accession number: P37298
Secondary accession number(s): D6VSG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health