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Protein

Phosphatidylinositol 4-kinase STT4

Gene

STT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. STT4 functions in PKC1 protein kinase pathway.

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

GO - Molecular functioni

  • 1-phosphatidylinositol 4-kinase activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • cortical actin cytoskeleton organization Source: CACAO
  • macroautophagy Source: SGD
  • mitophagy Source: SGD
  • phosphatidylinositol-mediated signaling Source: InterPro
  • phosphatidylinositol phosphorylation Source: SGD
  • regulation of fungal-type cell wall organization Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-365.
ReactomeiR-SCE-1483248. Synthesis of PIPs at the ER membrane.
R-SCE-1660514. Synthesis of PIPs at the Golgi membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase STT4 (EC:2.7.1.67)
Short name:
PI4-kinase
Short name:
PtdIns-4-kinase
Gene namesi
Name:STT4
Ordered Locus Names:YLR305C
ORF Names:L2142.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR305C.
SGDiS000004296. STT4.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000888351 – 1900Phosphatidylinositol 4-kinase STT4Add BLAST1900

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei459PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP37297.
PRIDEiP37297.

PTM databases

iPTMnetiP37297.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
YPP1P469512EBI-18454,EBI-23455

Protein-protein interaction databases

BioGridi31570. 52 interactors.
DIPiDIP-1677N.
IntActiP37297. 13 interactors.
MINTiMINT-388790.

Structurei

3D structure databases

ProteinModelPortaliP37297.
SMRiP37297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1345 – 1530PIK helicalPROSITE-ProRule annotationAdd BLAST186
Domaini1643 – 1882PI3K/PI4KPROSITE-ProRule annotationAdd BLAST240

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1531 – 1648Pleckstrin homology (PH) domain conferring phosphoinositide binding specificityBy similarityAdd BLAST118

Sequence similaritiesi

Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074798.
HOGENOMiHOG000179764.
InParanoidiP37297.
KOiK00888.
OMAiIRFQEAR.
OrthoDBiEOG092C0K2T.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 2 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFTRGLKAS SSLRAKAIGR LTKLSTGAPN DQNSNGTTLD LITHTLPIFY
60 70 80 90 100
STNTSKIYTI PLTLSEWEVL TSLCVAIPTT LDLVETMLKE IIAPYFLETP
110 120 130 140 150
RQRISDVLSS KFKLEQMRNP IELLTFQLTK FMIQACEQYP VLYENIGGII
160 170 180 190 200
STYFERVLKI FTIKQSGLLS LVGFINAFIQ FPNSTELTKF TWKKLAKLVL
210 220 230 240 250
RGSFLNEVDK ILNSSATFTN DSIVQYYDAG NELSSAYLLE LISRLQVSLI
260 270 280 290 300
SHLLNTSHVG ANLSEFLLNQ QYQFYKFDQE VADENDDTKC IDDFFFNVRS
310 320 330 340 350
NKQFFTDMCK ISLQFCSESH ILDLSTDNRA RFSFDTRAHY LQTLCLIPFI
360 370 380 390 400
EDTESELFES FTNVVSESID KFFLSDVVTP SLIKAIVASA SLLNFFTEKL
410 420 430 440 450
SLTLIRMFPL LVASPHITTE TVNDVAKIFT TGLYPLNEDA IVSTIYSMNN
460 470 480 490 500
LLAVSEDGSP VPVLRERQLT ITSGKNIEKD YFPLRNSSAS LDGTGALLGN
510 520 530 540 550
TTVGQLSSHD VNSGATMTYH ASLISNCVAA TTTIASYYNT QSITALTISI
560 570 580 590 600
LTQKVNSMSK ELDGVILNSL ARLAPNTSLT EFSLLLKFFK SRTVIATKID
610 620 630 640 650
DSALLKNIIK AKCVISKELL ARHFSSDLYF MYLHDLLDSI IASGEVERLE
660 670 680 690 700
HHRPQTEISR VADQIATYLE PLAALLPVPG DTPLDINKDE VTTNKFRNAW
710 720 730 740 750
FNFVIHGYHL GGPIVKRNFS FLLTIAYNSP PLASEFPANN KELSLEMNTI
760 770 780 790 800
LRRGSSNENI KQQKQQITEY FNTNIVQYRT TSSSKIMFLA AAVLLETIRC
810 820 830 840 850
EAGDCSKTLL YFSDPSILSG SIEKCIAVLS VSMIRKYARL IQKGNDAIFN
860 870 880 890 900
SKMIAQQLNN LLLCLSHREP TLQDAAFHAC EIFIRSIPSS LCHHLSLYTL
910 920 930 940 950
LDMLTALFDS ILDSEAHKFE PRYEFKLKHS KTTILVPSSS SWRATTLSRL
960 970 980 990 1000
HKSAKEWVRI LLNRSNQDTK ILLQSYISDL GEYSRLNSVE FGVSFAMDMA
1010 1020 1030 1040 1050
GLILPADKEL SRLTYYGPEK PNTISGFISL HSWRSKYLFD TAITSSPEDI
1060 1070 1080 1090 1100
KRQIGISTQN IRKNLTLGNK IITKDVTDFL DMATALLILG NGAPASLIYD
1110 1120 1130 1140 1150
IVHIPFEVFT SASLKIATNV WLTIITEKPE VAHLLLVEVC YCWMRSIDDN
1160 1170 1180 1190 1200
IGLYSRDHDL KGEEYQKMEY SPYDKAGINR DAKNASQAMQ PHLHVIKFFA
1210 1220 1230 1240 1250
SHFEGTLFQS DFLLKIFTKC ALYGIKNLYK ASLHPFARMI RHELLLFATL
1260 1270 1280 1290 1300
VLNASYKQGS KYMGRLSQEI TNGALSWFKR PVAWPFGSNE LKIKADLSVT
1310 1320 1330 1340 1350
RDLFLQLNKL SSLMSRHCGK DYKILNYFLA SEIQQIQTWL TPTEKIEGAD
1360 1370 1380 1390 1400
SNELTSDIVE ATFAKDPTLA INLLQRCYSK KAEDVLVGLV AKHALMCVGS
1410 1420 1430 1440 1450
PSALDLFIKG SHLSSKKDLH ATLYWAPVSP LKSINLFLPE WQGNSFILQF
1460 1470 1480 1490 1500
SIYSLESQDV NLAFFYVPQI VQCLRYDKTG YVERLILDTA KISVLFSHQI
1510 1520 1530 1540 1550
IWNMLANCYK DDEGIQEDEI KPTLDRIRER MVSSFSQSHR DFYEREFEFF
1560 1570 1580 1590 1600
DEVTGISGKL KPYIKKSKAE KKHKIDEEMS KIEVKPDVYL PSNPDGVVID
1610 1620 1630 1640 1650
IDRKSGKPLQ SHAKAPFMAT FKIKKDVKDP LTGKNKEVEK WQAAIFKVGD
1660 1670 1680 1690 1700
DCRQDVLALQ LISLFRTIWS SIGLDVYVFP YRVTATAPGC GVIDVLPNSV
1710 1720 1730 1740 1750
SRDMLGREAV NGLYEYFTSK FGNESTIEFQ NARNNFVKSL AGYSVISYLL
1760 1770 1780 1790 1800
QFKDRHNGNI MYDDQGHCLH IDFGFIFDIV PGGIKFEAVP FKLTKEMVKV
1810 1820 1830 1840 1850
MGGSPQTPAY LDFEELCIKA YLAARPHVEA IIECVNPMLG SGLPCFKGHK
1860 1870 1880 1890 1900
TIRNLRARFQ PQKTDHEAAL YMKALIRKSY ESIFTKGYDE FQRLTNGIPY
Length:1,900
Mass (Da):214,607
Last modified:October 1, 1994 - v1
Checksum:iF210BAF987BA276A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13717 Genomic DNA. Translation: BAA02870.1.
U17247 Genomic DNA. Translation: AAB67358.1.
U17243 Genomic DNA. Translation: AAB67354.1.
BK006945 Genomic DNA. Translation: DAA09614.1.
PIRiS45530.
RefSeqiNP_013408.1. NM_001182193.1.

Genome annotation databases

EnsemblFungiiYLR305C; YLR305C; YLR305C.
GeneIDi851014.
KEGGisce:YLR305C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13717 Genomic DNA. Translation: BAA02870.1.
U17247 Genomic DNA. Translation: AAB67358.1.
U17243 Genomic DNA. Translation: AAB67354.1.
BK006945 Genomic DNA. Translation: DAA09614.1.
PIRiS45530.
RefSeqiNP_013408.1. NM_001182193.1.

3D structure databases

ProteinModelPortaliP37297.
SMRiP37297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31570. 52 interactors.
DIPiDIP-1677N.
IntActiP37297. 13 interactors.
MINTiMINT-388790.

PTM databases

iPTMnetiP37297.

Proteomic databases

MaxQBiP37297.
PRIDEiP37297.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR305C; YLR305C; YLR305C.
GeneIDi851014.
KEGGisce:YLR305C.

Organism-specific databases

EuPathDBiFungiDB:YLR305C.
SGDiS000004296. STT4.

Phylogenomic databases

GeneTreeiENSGT00550000074798.
HOGENOMiHOG000179764.
InParanoidiP37297.
KOiK00888.
OMAiIRFQEAR.
OrthoDBiEOG092C0K2T.

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-365.
ReactomeiR-SCE-1483248. Synthesis of PIPs at the ER membrane.
R-SCE-1660514. Synthesis of PIPs at the Golgi membrane.

Miscellaneous databases

PROiP37297.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 2 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTT4_YEAST
AccessioniPrimary (citable) accession number: P37297
Secondary accession number(s): D6VYU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.