P37297 (STT4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-kinase STT4
Short name=PI4-kinase
Short name=PtdIns-4-kinase
EC=2.7.1.67

Gene names

Name:	STT4
Ordered Locus Names:	YLR305C
ORF Names:	L2142.4

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1900 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. STT4 functions in PKC1 protein kinase pathway.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Miscellaneous	Present with 846 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: SGD plasma membrane Inferred from direct assay. Source: SGD
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from direct assay. Source: SGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YPP1	P46951	2	EBI-18454,EBI-23455

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1900	1900	Phosphatidylinositol 4-kinase STT4		PRO_0000088835
Regions
	Domain	1345 – 1530	186	PIK helical
	Domain	1643 – 1882	240	PI3K/PI4K
	Region	1531 – 1648	118	Pleckstrin homology (PH) domain conferring phosphoinositide binding specificity By similarity
Amino acid modifications
	Modified residue	459	1	Phosphoserine Ref.5
	Modified residue	473	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P37297 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: F210BAF987BA276A	FASTA	1,900	214,607
	10 20 30 40 50 60 MRFTRGLKAS SSLRAKAIGR LTKLSTGAPN DQNSNGTTLD LITHTLPIFY STNTSKIYTI 70 80 90 100 110 120 PLTLSEWEVL TSLCVAIPTT LDLVETMLKE IIAPYFLETP RQRISDVLSS KFKLEQMRNP 130 140 150 160 170 180 IELLTFQLTK FMIQACEQYP VLYENIGGII STYFERVLKI FTIKQSGLLS LVGFINAFIQ 190 200 210 220 230 240 FPNSTELTKF TWKKLAKLVL RGSFLNEVDK ILNSSATFTN DSIVQYYDAG NELSSAYLLE 250 260 270 280 290 300 LISRLQVSLI SHLLNTSHVG ANLSEFLLNQ QYQFYKFDQE VADENDDTKC IDDFFFNVRS 310 320 330 340 350 360 NKQFFTDMCK ISLQFCSESH ILDLSTDNRA RFSFDTRAHY LQTLCLIPFI EDTESELFES 370 380 390 400 410 420 FTNVVSESID KFFLSDVVTP SLIKAIVASA SLLNFFTEKL SLTLIRMFPL LVASPHITTE 430 440 450 460 470 480 TVNDVAKIFT TGLYPLNEDA IVSTIYSMNN LLAVSEDGSP VPVLRERQLT ITSGKNIEKD 490 500 510 520 530 540 YFPLRNSSAS LDGTGALLGN TTVGQLSSHD VNSGATMTYH ASLISNCVAA TTTIASYYNT 550 560 570 580 590 600 QSITALTISI LTQKVNSMSK ELDGVILNSL ARLAPNTSLT EFSLLLKFFK SRTVIATKID 610 620 630 640 650 660 DSALLKNIIK AKCVISKELL ARHFSSDLYF MYLHDLLDSI IASGEVERLE HHRPQTEISR 670 680 690 700 710 720 VADQIATYLE PLAALLPVPG DTPLDINKDE VTTNKFRNAW FNFVIHGYHL GGPIVKRNFS 730 740 750 760 770 780 FLLTIAYNSP PLASEFPANN KELSLEMNTI LRRGSSNENI KQQKQQITEY FNTNIVQYRT 790 800 810 820 830 840 TSSSKIMFLA AAVLLETIRC EAGDCSKTLL YFSDPSILSG SIEKCIAVLS VSMIRKYARL 850 860 870 880 890 900 IQKGNDAIFN SKMIAQQLNN LLLCLSHREP TLQDAAFHAC EIFIRSIPSS LCHHLSLYTL 910 920 930 940 950 960 LDMLTALFDS ILDSEAHKFE PRYEFKLKHS KTTILVPSSS SWRATTLSRL HKSAKEWVRI 970 980 990 1000 1010 1020 LLNRSNQDTK ILLQSYISDL GEYSRLNSVE FGVSFAMDMA GLILPADKEL SRLTYYGPEK 1030 1040 1050 1060 1070 1080 PNTISGFISL HSWRSKYLFD TAITSSPEDI KRQIGISTQN IRKNLTLGNK IITKDVTDFL 1090 1100 1110 1120 1130 1140 DMATALLILG NGAPASLIYD IVHIPFEVFT SASLKIATNV WLTIITEKPE VAHLLLVEVC 1150 1160 1170 1180 1190 1200 YCWMRSIDDN IGLYSRDHDL KGEEYQKMEY SPYDKAGINR DAKNASQAMQ PHLHVIKFFA 1210 1220 1230 1240 1250 1260 SHFEGTLFQS DFLLKIFTKC ALYGIKNLYK ASLHPFARMI RHELLLFATL VLNASYKQGS 1270 1280 1290 1300 1310 1320 KYMGRLSQEI TNGALSWFKR PVAWPFGSNE LKIKADLSVT RDLFLQLNKL SSLMSRHCGK 1330 1340 1350 1360 1370 1380 DYKILNYFLA SEIQQIQTWL TPTEKIEGAD SNELTSDIVE ATFAKDPTLA INLLQRCYSK 1390 1400 1410 1420 1430 1440 KAEDVLVGLV AKHALMCVGS PSALDLFIKG SHLSSKKDLH ATLYWAPVSP LKSINLFLPE 1450 1460 1470 1480 1490 1500 WQGNSFILQF SIYSLESQDV NLAFFYVPQI VQCLRYDKTG YVERLILDTA KISVLFSHQI 1510 1520 1530 1540 1550 1560 IWNMLANCYK DDEGIQEDEI KPTLDRIRER MVSSFSQSHR DFYEREFEFF DEVTGISGKL 1570 1580 1590 1600 1610 1620 KPYIKKSKAE KKHKIDEEMS KIEVKPDVYL PSNPDGVVID IDRKSGKPLQ SHAKAPFMAT 1630 1640 1650 1660 1670 1680 FKIKKDVKDP LTGKNKEVEK WQAAIFKVGD DCRQDVLALQ LISLFRTIWS SIGLDVYVFP 1690 1700 1710 1720 1730 1740 YRVTATAPGC GVIDVLPNSV SRDMLGREAV NGLYEYFTSK FGNESTIEFQ NARNNFVKSL 1750 1760 1770 1780 1790 1800 AGYSVISYLL QFKDRHNGNI MYDDQGHCLH IDFGFIFDIV PGGIKFEAVP FKLTKEMVKV 1810 1820 1830 1840 1850 1860 MGGSPQTPAY LDFEELCIKA YLAARPHVEA IIECVNPMLG SGLPCFKGHK TIRNLRARFQ 1870 1880 1890 1900 PQKTDHEAAL YMKALIRKSY ESIFTKGYDE FQRLTNGIPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae." Yoshida S., Goebl M., Ohya Y., Nakano A., Anraku Y. J. Biol. Chem. 269:1166-1172(1994) [PubMed: 8288577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459 AND SER-473, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13717 Genomic DNA. Translation: BAA02870.1. U17247 Genomic DNA. Translation: AAB67358.1. U17243 Genomic DNA. Translation: AAB67354.1. BK006945 Genomic DNA. Translation: DAA09614.1.
PIR	S45530.
RefSeq	NP_013408.1. NM_001182193.1.
3D structure databases
ProteinModelPortal	P37297.
SMR	P37297. Positions 1373-1890.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1677N.
IntAct	P37297. 13 interactions.
MINT	MINT-388790.
STRING	P37297.
Proteomic databases
PeptideAtlas	P37297.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR305C; YLR305C; YLR305C.
GeneID	851014.
KEGG	sce:YLR305C.
NMPDR	fig|4932.3.peg.4426.
Organism-specific databases
CYGD	YLR305c.
SGD	S000004296. STT4.
Phylogenomic databases
eggNOG	fuNOG06080.
GeneTree	EFGT00050000002040.
HOGENOM	HBG325491.
OMA	CGVIDVL.
OrthoDB	EOG4GF6P8.
Gene expression databases
ArrayExpress	P37297.
Genevestigator	P37297.
GermOnline	YLR305C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit. G3DSA:1.25.40.70. PI3Ka. 1 hit.
KO	K00888.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
SMART	SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967574.

Entry information

Entry name

STT4_YEAST

Accession

Primary (citable) accession number: P37297
Secondary accession number(s): D6VYU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program