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P37296

- STV1_YEAST

UniProt

P37296 - STV1_YEAST

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Protein

V-type proton ATPase subunit a, Golgi isoform

Gene

STV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in Golgi- and endosome-residing V-ATPase complexes. Enzymes containing this subunit have a 4-fold lower ratio of proton transport to ATP hydrolysis than complexes containing the vacuolar isoform and do not dissociate V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.2 Publications

GO - Molecular functioni

  1. proton-transporting ATPase activity, rotational mechanism Source: SGD

GO - Biological processi

  1. ATP hydrolysis coupled proton transport Source: InterPro
  2. vacuolar acidification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32759-MONOMER.
ReactomeiREACT_189031. Phagosomal maturation (early endosomal stage).
REACT_189190. Transferrin endocytosis and recycling.
REACT_255567. Insulin receptor recycling.

Protein family/group databases

TCDBi3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase subunit a, Golgi isoform
Short name:
V-ATPase a 2 subunit
Alternative name(s):
Similar to VPH1 protein 1
V-ATPase 101 kDa subunit
V-ATPase subunit AC115
Vacuolar proton translocating ATPase subunit a 2
Gene namesi
Name:STV1
Ordered Locus Names:YMR054W
ORF Names:YM9796.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR054w.
SGDiS000004658. STV1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 450450CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei451 – 46919HelicalSequence AnalysisAdd
BLAST
Topological domaini470 – 4712VacuolarSequence Analysis
Transmembranei472 – 48817HelicalSequence AnalysisAdd
BLAST
Topological domaini489 – 50214CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei503 – 53230HelicalSequence AnalysisAdd
BLAST
Topological domaini533 – 58048VacuolarSequence AnalysisAdd
BLAST
Transmembranei581 – 60020HelicalSequence AnalysisAdd
BLAST
Topological domaini601 – 61818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei619 – 63921HelicalSequence AnalysisAdd
BLAST
Topological domaini640 – 68243VacuolarSequence AnalysisAdd
BLAST
Transmembranei683 – 70220HelicalSequence AnalysisAdd
BLAST
Topological domaini703 – 77977CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei780 – 80425HelicalSequence AnalysisAdd
BLAST
Topological domaini805 – 82824VacuolarSequence AnalysisAdd
BLAST
Transmembranei829 – 86739HelicalSequence AnalysisAdd
BLAST
Topological domaini868 – 89023CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. late endosome Source: SGD
  4. vacuolar proton-transporting V-type ATPase, V0 domain Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 890890V-type proton ATPase subunit a, Golgi isoformPRO_0000119225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei223 – 2231Phosphoserine3 Publications
Modified residuei228 – 2281Phosphoserine1 Publication

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP37296.
PaxDbiP37296.

Expressioni

Gene expression databases

GenevestigatoriP37296.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e).

Protein-protein interaction databases

BioGridi35230. 90 interactions.
DIPiDIP-4450N.
IntActiP37296. 2 interactions.
MINTiMINT-568679.
STRINGi4932.YMR054W.

Structurei

3D structure databases

ProteinModelPortaliP37296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili113 – 15442Sequence AnalysisAdd
BLAST
Coiled coili297 – 34751Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the V-ATPase 116 kDa subunit family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1269.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
InParanoidiP37296.
KOiK02154.
OMAiTSEIRRC.
OrthoDBiEOG7TJ3S8.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.

Sequencei

Sequence statusi: Complete.

P37296-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQEEAIFRS ADMTYVQLYI PLEVIREVTF LLGKMSVFMV MDLNKDLTAF
60 70 80 90 100
QRGYVNQLRR FDEVERMVGF LNEVVEKHAA ETWKYILHID DEGNDIAQPD
110 120 130 140 150
MADLINTMEP LSLENVNDMV KEITDCESRA RQLDESLDSL RSKLNDLLEQ
160 170 180 190 200
RQVIFECSKF IEVNPGIAGR ATNPEIEQEE RDVDEFRMTP DDISETLSDA
210 220 230 240 250
FSFDDETPQD RGALGNDLTR NQSVEDLSFL EQGYQHRYMI TGSIRRTKVD
260 270 280 290 300
ILNRILWRLL RGNLIFQNFP IEEPLLEGKE KVEKDCFIIF THGETLLKKV
310 320 330 340 350
KRVIDSLNGK IVSLNTRSSE LVDTLNRQID DLQRILDTTE QTLHTELLVI
360 370 380 390 400
HDQLPVWSAM TKREKYVYTT LNKFQQESQG LIAEGWVPST ELIHLQDSLK
410 420 430 440 450
DYIETLGSEY STVFNVILTN KLPPTYHRTN KFTQAFQSIV DAYGIATYKE
460 470 480 490 500
INAGLATVVT FPFMFAIMFG DMGHGFILFL MALFLVLNER KFGAMHRDEI
510 520 530 540 550
FDMAFTGRYV LLLMGAFSVY TGLLYNDIFS KSMTIFKSGW QWPSTFRKGE
560 570 580 590 600
SIEAKKTGVY PFGLDFAWHG TDNGLLFSNS YKMKLSILMG YAHMTYSFMF
610 620 630 640 650
SYINYRAKNS KVDIIGNFIP GLVFMQSIFG YLSWAIVYKW SKDWIKDDKP
660 670 680 690 700
APGLLNMLIN MFLAPGTIDD QLYSGQAKLQ VVLLLAALVC VPWLLLYKPL
710 720 730 740 750
TLRRLNKNGG GGRPHGYQSV GNIEHEEQIA QQRHSAEGFQ GMIISDVASV
760 770 780 790 800
ADSINESVGG GEQGPFNFGD VMIHQVIHTI EFCLNCISHT ASYLRLWALS
810 820 830 840 850
LAHAQLSSVL WDMTISNAFS SKNSGSPLAV MKVVFLFAMW FVLTVCILVF
860 870 880 890
MEGTSAMLHA LRLHWVEAMS KFFEGEGYAY EPFSFRAIIE
Length:890
Mass (Da):101,661
Last modified:October 1, 1996 - v2
Checksum:i7CA12E37C1E42C67
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti805 – 8051Q → E in AAA20596. (PubMed:7514599)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06465 Genomic DNA. Translation: AAA20596.1.
Z49703 Genomic DNA. Translation: CAA89764.1.
BK006946 Genomic DNA. Translation: DAA09953.1.
PIRiS54554.
RefSeqiNP_013770.1. NM_001182552.1.

Genome annotation databases

EnsemblFungiiYMR054W; YMR054W; YMR054W.
GeneIDi855076.
KEGGisce:YMR054W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06465 Genomic DNA. Translation: AAA20596.1 .
Z49703 Genomic DNA. Translation: CAA89764.1 .
BK006946 Genomic DNA. Translation: DAA09953.1 .
PIRi S54554.
RefSeqi NP_013770.1. NM_001182552.1.

3D structure databases

ProteinModelPortali P37296.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35230. 90 interactions.
DIPi DIP-4450N.
IntActi P37296. 2 interactions.
MINTi MINT-568679.
STRINGi 4932.YMR054W.

Protein family/group databases

TCDBi 3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

MaxQBi P37296.
PaxDbi P37296.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR054W ; YMR054W ; YMR054W .
GeneIDi 855076.
KEGGi sce:YMR054W.

Organism-specific databases

CYGDi YMR054w.
SGDi S000004658. STV1.

Phylogenomic databases

eggNOGi COG1269.
GeneTreei ENSGT00390000004941.
HOGENOMi HOG000037059.
InParanoidi P37296.
KOi K02154.
OMAi TSEIRRC.
OrthoDBi EOG7TJ3S8.

Enzyme and pathway databases

BioCyci YEAST:G3O-32759-MONOMER.
Reactomei REACT_189031. Phagosomal maturation (early endosomal stage).
REACT_189190. Transferrin endocytosis and recycling.
REACT_255567. Insulin receptor recycling.

Miscellaneous databases

NextBioi 978354.
PROi P37296.

Gene expression databases

Genevestigatori P37296.

Family and domain databases

InterProi IPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view ]
PANTHERi PTHR11629. PTHR11629. 1 hit.
Pfami PF01496. V_ATPase_I. 1 hit.
[Graphical view ]
PIRSFi PIRSF001293. ATP6V0A1. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p."
    Manolson M.F., Wu B., Proteau D., Taillon B.E., Roberts B.T., Hoyt M.A., Jones E.W.
    J. Biol. Chem. 269:14064-14074(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: ATCC 26109 / X2180.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation."
    Kawasaki-Nishi S., Nishi T., Forgac M.
    J. Biol. Chem. 276:17941-17948(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223 AND SER-228, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTV1_YEAST
AccessioniPrimary (citable) accession number: P37296
Secondary accession number(s): D6VZM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1084 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3