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P37296

- STV1_YEAST

UniProt

P37296 - STV1_YEAST

Protein

V-type proton ATPase subunit a, Golgi isoform

Gene

STV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in Golgi- and endosome-residing V-ATPase complexes. Enzymes containing this subunit have a 4-fold lower ratio of proton transport to ATP hydrolysis than complexes containing the vacuolar isoform and do not dissociate V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.2 Publications

    GO - Molecular functioni

    1. proton-transporting ATPase activity, rotational mechanism Source: SGD

    GO - Biological processi

    1. ATP hydrolysis coupled proton transport Source: InterPro
    2. vacuolar acidification Source: SGD

    Keywords - Biological processi

    Hydrogen ion transport, Ion transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32759-MONOMER.
    ReactomeiREACT_189031. Phagosomal maturation (early endosomal stage).
    REACT_189190. Transferrin endocytosis and recycling.

    Protein family/group databases

    TCDBi3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    V-type proton ATPase subunit a, Golgi isoform
    Short name:
    V-ATPase a 2 subunit
    Alternative name(s):
    Similar to VPH1 protein 1
    V-ATPase 101 kDa subunit
    V-ATPase subunit AC115
    Vacuolar proton translocating ATPase subunit a 2
    Gene namesi
    Name:STV1
    Ordered Locus Names:YMR054W
    ORF Names:YM9796.07
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR054w.
    SGDiS000004658. STV1.

    Subcellular locationi

    GO - Cellular componenti

    1. endosome membrane Source: UniProtKB-SubCell
    2. Golgi apparatus Source: SGD
    3. Golgi membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. late endosome Source: SGD
    6. vacuolar proton-transporting V-type ATPase, V0 domain Source: SGD

    Keywords - Cellular componenti

    Endosome, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 890890V-type proton ATPase subunit a, Golgi isoformPRO_0000119225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei223 – 2231Phosphoserine3 Publications
    Modified residuei228 – 2281Phosphoserine1 Publication

    Post-translational modificationi

    Glycosylated.

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP37296.
    PaxDbiP37296.

    Expressioni

    Gene expression databases

    GenevestigatoriP37296.

    Interactioni

    Subunit structurei

    V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e).

    Protein-protein interaction databases

    BioGridi35230. 89 interactions.
    DIPiDIP-4450N.
    IntActiP37296. 2 interactions.
    MINTiMINT-568679.
    STRINGi4932.YMR054W.

    Structurei

    3D structure databases

    ProteinModelPortaliP37296.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 450450CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini470 – 4712VacuolarSequence Analysis
    Topological domaini489 – 50214CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini533 – 58048VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini601 – 61818CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini640 – 68243VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini703 – 77977CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini805 – 82824VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini868 – 89023CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei451 – 46919HelicalSequence AnalysisAdd
    BLAST
    Transmembranei472 – 48817HelicalSequence AnalysisAdd
    BLAST
    Transmembranei503 – 53230HelicalSequence AnalysisAdd
    BLAST
    Transmembranei581 – 60020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei619 – 63921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei683 – 70220HelicalSequence AnalysisAdd
    BLAST
    Transmembranei780 – 80425HelicalSequence AnalysisAdd
    BLAST
    Transmembranei829 – 86739HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili113 – 15442Sequence AnalysisAdd
    BLAST
    Coiled coili297 – 34751Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the V-ATPase 116 kDa subunit family.Curated

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1269.
    GeneTreeiENSGT00390000004941.
    HOGENOMiHOG000037059.
    KOiK02154.
    OMAiTSEIRRC.
    OrthoDBiEOG7TJ3S8.

    Family and domain databases

    InterProiIPR002490. V-ATPase_116kDa_su.
    IPR026028. V-type_ATPase_116kDa_su_euka.
    [Graphical view]
    PANTHERiPTHR11629. PTHR11629. 1 hit.
    PfamiPF01496. V_ATPase_I. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001293. ATP6V0A1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37296-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNQEEAIFRS ADMTYVQLYI PLEVIREVTF LLGKMSVFMV MDLNKDLTAF    50
    QRGYVNQLRR FDEVERMVGF LNEVVEKHAA ETWKYILHID DEGNDIAQPD 100
    MADLINTMEP LSLENVNDMV KEITDCESRA RQLDESLDSL RSKLNDLLEQ 150
    RQVIFECSKF IEVNPGIAGR ATNPEIEQEE RDVDEFRMTP DDISETLSDA 200
    FSFDDETPQD RGALGNDLTR NQSVEDLSFL EQGYQHRYMI TGSIRRTKVD 250
    ILNRILWRLL RGNLIFQNFP IEEPLLEGKE KVEKDCFIIF THGETLLKKV 300
    KRVIDSLNGK IVSLNTRSSE LVDTLNRQID DLQRILDTTE QTLHTELLVI 350
    HDQLPVWSAM TKREKYVYTT LNKFQQESQG LIAEGWVPST ELIHLQDSLK 400
    DYIETLGSEY STVFNVILTN KLPPTYHRTN KFTQAFQSIV DAYGIATYKE 450
    INAGLATVVT FPFMFAIMFG DMGHGFILFL MALFLVLNER KFGAMHRDEI 500
    FDMAFTGRYV LLLMGAFSVY TGLLYNDIFS KSMTIFKSGW QWPSTFRKGE 550
    SIEAKKTGVY PFGLDFAWHG TDNGLLFSNS YKMKLSILMG YAHMTYSFMF 600
    SYINYRAKNS KVDIIGNFIP GLVFMQSIFG YLSWAIVYKW SKDWIKDDKP 650
    APGLLNMLIN MFLAPGTIDD QLYSGQAKLQ VVLLLAALVC VPWLLLYKPL 700
    TLRRLNKNGG GGRPHGYQSV GNIEHEEQIA QQRHSAEGFQ GMIISDVASV 750
    ADSINESVGG GEQGPFNFGD VMIHQVIHTI EFCLNCISHT ASYLRLWALS 800
    LAHAQLSSVL WDMTISNAFS SKNSGSPLAV MKVVFLFAMW FVLTVCILVF 850
    MEGTSAMLHA LRLHWVEAMS KFFEGEGYAY EPFSFRAIIE 890
    Length:890
    Mass (Da):101,661
    Last modified:October 1, 1996 - v2
    Checksum:i7CA12E37C1E42C67
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti805 – 8051Q → E in AAA20596. (PubMed:7514599)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U06465 Genomic DNA. Translation: AAA20596.1.
    Z49703 Genomic DNA. Translation: CAA89764.1.
    BK006946 Genomic DNA. Translation: DAA09953.1.
    PIRiS54554.
    RefSeqiNP_013770.1. NM_001182552.1.

    Genome annotation databases

    EnsemblFungiiYMR054W; YMR054W; YMR054W.
    GeneIDi855076.
    KEGGisce:YMR054W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U06465 Genomic DNA. Translation: AAA20596.1 .
    Z49703 Genomic DNA. Translation: CAA89764.1 .
    BK006946 Genomic DNA. Translation: DAA09953.1 .
    PIRi S54554.
    RefSeqi NP_013770.1. NM_001182552.1.

    3D structure databases

    ProteinModelPortali P37296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35230. 89 interactions.
    DIPi DIP-4450N.
    IntActi P37296. 2 interactions.
    MINTi MINT-568679.
    STRINGi 4932.YMR054W.

    Protein family/group databases

    TCDBi 3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Proteomic databases

    MaxQBi P37296.
    PaxDbi P37296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR054W ; YMR054W ; YMR054W .
    GeneIDi 855076.
    KEGGi sce:YMR054W.

    Organism-specific databases

    CYGDi YMR054w.
    SGDi S000004658. STV1.

    Phylogenomic databases

    eggNOGi COG1269.
    GeneTreei ENSGT00390000004941.
    HOGENOMi HOG000037059.
    KOi K02154.
    OMAi TSEIRRC.
    OrthoDBi EOG7TJ3S8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32759-MONOMER.
    Reactomei REACT_189031. Phagosomal maturation (early endosomal stage).
    REACT_189190. Transferrin endocytosis and recycling.

    Miscellaneous databases

    NextBioi 978354.
    PROi P37296.

    Gene expression databases

    Genevestigatori P37296.

    Family and domain databases

    InterProi IPR002490. V-ATPase_116kDa_su.
    IPR026028. V-type_ATPase_116kDa_su_euka.
    [Graphical view ]
    PANTHERi PTHR11629. PTHR11629. 1 hit.
    Pfami PF01496. V_ATPase_I. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001293. ATP6V0A1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p."
      Manolson M.F., Wu B., Proteau D., Taillon B.E., Roberts B.T., Hoyt M.A., Jones E.W.
      J. Biol. Chem. 269:14064-14074(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: ATCC 26109 / X2180.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation."
      Kawasaki-Nishi S., Nishi T., Forgac M.
      J. Biol. Chem. 276:17941-17948(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223 AND SER-228, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSTV1_YEAST
    AccessioniPrimary (citable) accession number: P37296
    Secondary accession number(s): D6VZM9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1084 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3