Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

V-type proton ATPase subunit a, Golgi isoform

Gene

STV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in Golgi- and endosome-residing V-ATPase complexes. Enzymes containing this subunit have a 4-fold lower ratio of proton transport to ATP hydrolysis than complexes containing the vacuolar isoform and do not dissociate V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.2 Publications

GO - Molecular functioni

  • ATPase binding Source: GO_Central
  • proton-transporting ATPase activity, rotational mechanism Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32759-MONOMER.
ReactomeiR-SCE-1222556. ROS, RNS production in response to bacteria.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-77387. Insulin receptor recycling.
R-SCE-917977. Transferrin endocytosis and recycling.

Protein family/group databases

TCDBi3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase subunit a, Golgi isoform
Short name:
V-ATPase a 2 subunit
Alternative name(s):
Similar to VPH1 protein 1
V-ATPase 101 kDa subunit
V-ATPase subunit AC115
Vacuolar proton translocating ATPase subunit a 2
Gene namesi
Name:STV1
Ordered Locus Names:YMR054W
ORF Names:YM9796.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR054W.
SGDiS000004658. STV1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 450CytoplasmicSequence analysisAdd BLAST450
Transmembranei451 – 469HelicalSequence analysisAdd BLAST19
Topological domaini470 – 471VacuolarSequence analysis2
Transmembranei472 – 488HelicalSequence analysisAdd BLAST17
Topological domaini489 – 502CytoplasmicSequence analysisAdd BLAST14
Transmembranei503 – 532HelicalSequence analysisAdd BLAST30
Topological domaini533 – 580VacuolarSequence analysisAdd BLAST48
Transmembranei581 – 600HelicalSequence analysisAdd BLAST20
Topological domaini601 – 618CytoplasmicSequence analysisAdd BLAST18
Transmembranei619 – 639HelicalSequence analysisAdd BLAST21
Topological domaini640 – 682VacuolarSequence analysisAdd BLAST43
Transmembranei683 – 702HelicalSequence analysisAdd BLAST20
Topological domaini703 – 779CytoplasmicSequence analysisAdd BLAST77
Transmembranei780 – 804HelicalSequence analysisAdd BLAST25
Topological domaini805 – 828VacuolarSequence analysisAdd BLAST24
Transmembranei829 – 867HelicalSequence analysisAdd BLAST39
Topological domaini868 – 890CytoplasmicSequence analysisAdd BLAST23

GO - Cellular componenti

  • endosome membrane Source: UniProtKB-SubCell
  • fungal-type vacuole membrane Source: GO_Central
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • late endosome Source: SGD
  • vacuolar proton-transporting V-type ATPase, V0 domain Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192251 – 890V-type proton ATPase subunit a, Golgi isoformAdd BLAST890

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei223PhosphoserineCombined sources1
Modified residuei228PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP37296.
PRIDEiP37296.

PTM databases

iPTMnetiP37296.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e).

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35230. 102 interactors.
DIPiDIP-4450N.
IntActiP37296. 2 interactors.
MINTiMINT-568679.

Structurei

3D structure databases

ProteinModelPortaliP37296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili113 – 154Sequence analysisAdd BLAST42
Coiled coili297 – 347Sequence analysisAdd BLAST51

Sequence similaritiesi

Belongs to the V-ATPase 116 kDa subunit family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
InParanoidiP37296.
KOiK02154.
OMAiEVLWNMT.
OrthoDBiEOG092C0YCY.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 2 hits.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.

Sequencei

Sequence statusi: Complete.

P37296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQEEAIFRS ADMTYVQLYI PLEVIREVTF LLGKMSVFMV MDLNKDLTAF
60 70 80 90 100
QRGYVNQLRR FDEVERMVGF LNEVVEKHAA ETWKYILHID DEGNDIAQPD
110 120 130 140 150
MADLINTMEP LSLENVNDMV KEITDCESRA RQLDESLDSL RSKLNDLLEQ
160 170 180 190 200
RQVIFECSKF IEVNPGIAGR ATNPEIEQEE RDVDEFRMTP DDISETLSDA
210 220 230 240 250
FSFDDETPQD RGALGNDLTR NQSVEDLSFL EQGYQHRYMI TGSIRRTKVD
260 270 280 290 300
ILNRILWRLL RGNLIFQNFP IEEPLLEGKE KVEKDCFIIF THGETLLKKV
310 320 330 340 350
KRVIDSLNGK IVSLNTRSSE LVDTLNRQID DLQRILDTTE QTLHTELLVI
360 370 380 390 400
HDQLPVWSAM TKREKYVYTT LNKFQQESQG LIAEGWVPST ELIHLQDSLK
410 420 430 440 450
DYIETLGSEY STVFNVILTN KLPPTYHRTN KFTQAFQSIV DAYGIATYKE
460 470 480 490 500
INAGLATVVT FPFMFAIMFG DMGHGFILFL MALFLVLNER KFGAMHRDEI
510 520 530 540 550
FDMAFTGRYV LLLMGAFSVY TGLLYNDIFS KSMTIFKSGW QWPSTFRKGE
560 570 580 590 600
SIEAKKTGVY PFGLDFAWHG TDNGLLFSNS YKMKLSILMG YAHMTYSFMF
610 620 630 640 650
SYINYRAKNS KVDIIGNFIP GLVFMQSIFG YLSWAIVYKW SKDWIKDDKP
660 670 680 690 700
APGLLNMLIN MFLAPGTIDD QLYSGQAKLQ VVLLLAALVC VPWLLLYKPL
710 720 730 740 750
TLRRLNKNGG GGRPHGYQSV GNIEHEEQIA QQRHSAEGFQ GMIISDVASV
760 770 780 790 800
ADSINESVGG GEQGPFNFGD VMIHQVIHTI EFCLNCISHT ASYLRLWALS
810 820 830 840 850
LAHAQLSSVL WDMTISNAFS SKNSGSPLAV MKVVFLFAMW FVLTVCILVF
860 870 880 890
MEGTSAMLHA LRLHWVEAMS KFFEGEGYAY EPFSFRAIIE
Length:890
Mass (Da):101,661
Last modified:October 1, 1996 - v2
Checksum:i7CA12E37C1E42C67
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti805Q → E in AAA20596 (PubMed:7514599).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06465 Genomic DNA. Translation: AAA20596.1.
Z49703 Genomic DNA. Translation: CAA89764.1.
BK006946 Genomic DNA. Translation: DAA09953.1.
PIRiS54554.
RefSeqiNP_013770.1. NM_001182552.1.

Genome annotation databases

EnsemblFungiiYMR054W; YMR054W; YMR054W.
GeneIDi855076.
KEGGisce:YMR054W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06465 Genomic DNA. Translation: AAA20596.1.
Z49703 Genomic DNA. Translation: CAA89764.1.
BK006946 Genomic DNA. Translation: DAA09953.1.
PIRiS54554.
RefSeqiNP_013770.1. NM_001182552.1.

3D structure databases

ProteinModelPortaliP37296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35230. 102 interactors.
DIPiDIP-4450N.
IntActiP37296. 2 interactors.
MINTiMINT-568679.

Protein family/group databases

TCDBi3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

iPTMnetiP37296.

Proteomic databases

MaxQBiP37296.
PRIDEiP37296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR054W; YMR054W; YMR054W.
GeneIDi855076.
KEGGisce:YMR054W.

Organism-specific databases

EuPathDBiFungiDB:YMR054W.
SGDiS000004658. STV1.

Phylogenomic databases

GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
InParanoidiP37296.
KOiK02154.
OMAiEVLWNMT.
OrthoDBiEOG092C0YCY.

Enzyme and pathway databases

BioCyciYEAST:G3O-32759-MONOMER.
ReactomeiR-SCE-1222556. ROS, RNS production in response to bacteria.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-77387. Insulin receptor recycling.
R-SCE-917977. Transferrin endocytosis and recycling.

Miscellaneous databases

PROiP37296.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 2 hits.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTV1_YEAST
AccessioniPrimary (citable) accession number: P37296
Secondary accession number(s): D6VZM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1084 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.