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P37295

- LCPS_NEUCR

UniProt

P37295 - LCPS_NEUCR

Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

al-2

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and from lycopene to beta-carotene via the intermediate gamma-carotene and from 3,4-didehydrolycopene to torulene (lycopene cyclase). Torulene is further processed to the acidic carotenoid neurosporaxanthin. The cyclase preferentially catalyzes single cyclizations at only one end of the substrate to produce monocyclic carotenoids.2 Publications

    Catalytic activityi

    Carotenoid psi-end group = carotenoid beta-end group.
    2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
    2. intramolecular lyase activity Source: InterPro
    3. lycopene beta cyclase activity Source: UniProtKB
    4. phytoene synthase activity Source: UniProtKB

    GO - Biological processi

    1. carotene biosynthetic process Source: UniProtKB
    2. carotenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Transferase

    Keywords - Biological processi

    Carotenoid biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16129.
    UniPathwayiUPA00799; UER00773.
    UPA00802.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional lycopene cyclase/phytoene synthase
    Alternative name(s):
    Protein albino-2
    Including the following 2 domains:
    Lycopene beta-cyclase (EC:5.5.1.19)
    Alternative name(s):
    Carotene cyclase
    Lycopene cyclase
    Phytoene synthase (EC:2.5.1.32)
    Gene namesi
    Name:al-2
    ORF Names:B22I21.230, NCU00585
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 1, Linkage Group I

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi189 – 1891W → R in JA26; Abolishes lycopene cyclase activity and accumulates a polar precursor carotenoid of neurosporaxanthin. 1 Publication
    Mutagenesisi208 – 2081K → E in JA28; Abolishes lycopene cyclase activity and accumulates a polar precursor carotenoid of neurosporaxanthin. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 602602Bifunctional lycopene cyclase/phytoene synthasePRO_0000067440Add
    BLAST

    Expressioni

    Inductioni

    By blue light.

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU00585.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP37295.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei30 – 5021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei76 – 9621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei168 – 18821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei230 – 25021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 241241Lycopene beta-cyclaseAdd
    BLAST
    Regioni248 – 602355Phytoene synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
    In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1562.
    KOiK17841.
    OrthoDBiEOG7RBZJ2.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR017825. Lycopene_cyclase_dom.
    IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P37295-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYDYAFVHLK FTVPAAVLLT AIAYPILNRI HLIQTGFLVV VAFTAALPWD    50
    AYLIKHKVWS YPPEAIVGPR LLGIPFEELF FFVIQTYITA LVYILFNKPV 100
    LHALHLNNQQ NPPAWMRVVK VTGQVVLVAL SVWGWNAAQV HQETSYLGLI 150
    LVWACPFLLA IWTLAGRFIL SLPWYATVLP MFLPTFYLWA VDEFALHRGT 200
    WSIGSGTKLD FCLFGKLDIE EATFFLVTNM LIVGGMAAFD QYLAVIYAFP 250
    TLFPKVNRYP TTHMLLQSRL INTSRYDLER IEGLREAVER LRLKSRSFYL 300
    ANSLFSGRLR IDLILLYSFC RLADDLVDDA KSRREVLSWT AKLNHFLDLH 350
    YKDADATEDP KKKAERIDAY IKTAFPPCAY QALHLLPTHI LPPKPLYDLI 400
    KGFEMDSQFT FHGTSDSTDL QYPIADDKDL ENYAIYVAGT VGELCIALII 450
    YHCLPDMSDT QKRELETAAC RMGIALQYVN IARDIVVDAR IGRVYLPTTW 500
    LKKEGLTHKM VLENPEGPEV IERMRRRLLE NAFELYGGAR PEMQRIPSEA 550
    RGPMIGAVEN YMAIGRVLRE RKEGTVFVRM EGRATVPKRR RLSTLLRALY 600
    EQ 602
    Length:602
    Mass (Da):68,928
    Last modified:October 1, 1994 - v1
    Checksum:iDDFFBD725007E5C2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27652 Genomic DNA. Translation: AAA19428.1.
    BX842641 Genomic DNA. Translation: CAE76609.1.
    CM002236 Genomic DNA. Translation: EAA36489.3.
    PIRiA53583.
    RefSeqiXP_965725.2. XM_960632.2.
    UniGeneiNcr.25908.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000000733; EFNCRP00000000733; EFNCRG00000000733.
    GeneIDi3881954.
    KEGGincr:NCU00585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27652 Genomic DNA. Translation: AAA19428.1 .
    BX842641 Genomic DNA. Translation: CAE76609.1 .
    CM002236 Genomic DNA. Translation: EAA36489.3 .
    PIRi A53583.
    RefSeqi XP_965725.2. XM_960632.2.
    UniGenei Ncr.25908.

    3D structure databases

    ProteinModelPortali P37295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU00585.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000000733 ; EFNCRP00000000733 ; EFNCRG00000000733 .
    GeneIDi 3881954.
    KEGGi ncr:NCU00585.

    Phylogenomic databases

    eggNOGi COG1562.
    KOi K17841.
    OrthoDBi EOG7RBZJ2.

    Enzyme and pathway databases

    UniPathwayi UPA00799 ; UER00773 .
    UPA00802 .
    BioCyci MetaCyc:MONOMER-16129.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR017825. Lycopene_cyclase_dom.
    IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    TIGRFAMsi TIGR03462. CarR_dom_SF. 2 hits.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of al-2, the phytoene synthase gene of Neurospora crassa. Cloning, sequence analysis, and photoregulation."
      Schmidhauser T.J., Lauter F.-R., Schumacher M., Zhou W., Russo V.E.A., Yanofsky C.
      J. Biol. Chem. 269:12060-12066(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    4. "Mutants of the carotene cyclase domain of al-2 from Neurospora crassa."
      Arrach N., Schmidhauser T.J., Avalos J.
      Mol. Genet. Genomics 266:914-921(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TRP-189 AND LYS-208.
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    5. "The biotechnological potential of the al-2 gene from Neurospora crassa for the production of monocyclic keto hydroxy carotenoids."
      Sandmann G., Zhu C., Krubasik P., Fraser P.D.
      Biochim. Biophys. Acta 1761:1085-1092(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiLCPS_NEUCR
    AccessioniPrimary (citable) accession number: P37295
    Secondary accession number(s): Q7RVN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3