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P37295 (LCPS_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional lycopene cyclase/phytoene synthase
Alternative name(s):
Protein albino-2

Including the following 2 domains:

  1. Lycopene beta-cyclase
    EC=5.5.1.19
    Alternative name(s):
    Carotene cyclase
    Lycopene cyclase
  2. Phytoene synthase
    EC=2.5.1.32
Gene names
Name:al-2
ORF Names:B22I21.230, NCU00585
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and from lycopene to beta-carotene via the intermediate gamma-carotene and from 3,4-didehydrolycopene to torulene (lycopene cyclase). Torulene is further processed to the acidic carotenoid neurosporaxanthin. The cyclase preferentially catalyzes single cyclizations at only one end of the substrate to produce monocyclic carotenoids. Ref.4 Ref.5

Catalytic activity

Carotenoid psi-end group = carotenoid beta-end group.

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathway

Carotenoid biosynthesis; beta-carotene biosynthesis.

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Induction

By blue light.

Sequence similarities

In the N-terminal section; belongs to the lycopene beta-cyclase family.

In the C-terminal section; belongs to the phytoene/squalene synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602Bifunctional lycopene cyclase/phytoene synthase
PRO_0000067440

Regions

Transmembrane6 – 2621Helical; Potential
Transmembrane30 – 5021Helical; Potential
Transmembrane76 – 9621Helical; Potential
Transmembrane118 – 13821Helical; Potential
Transmembrane146 – 16621Helical; Potential
Transmembrane168 – 18821Helical; Potential
Transmembrane230 – 25021Helical; Potential
Region1 – 241241Lycopene beta-cyclase
Region248 – 602355Phytoene synthase

Experimental info

Mutagenesis1891W → R in JA26; Abolishes lycopene cyclase activity and accumulates a polar precursor carotenoid of neurosporaxanthin. Ref.4
Mutagenesis2081K → E in JA28; Abolishes lycopene cyclase activity and accumulates a polar precursor carotenoid of neurosporaxanthin. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P37295 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: DDFFBD725007E5C2

FASTA60268,928
        10         20         30         40         50         60 
MYDYAFVHLK FTVPAAVLLT AIAYPILNRI HLIQTGFLVV VAFTAALPWD AYLIKHKVWS 

        70         80         90        100        110        120 
YPPEAIVGPR LLGIPFEELF FFVIQTYITA LVYILFNKPV LHALHLNNQQ NPPAWMRVVK 

       130        140        150        160        170        180 
VTGQVVLVAL SVWGWNAAQV HQETSYLGLI LVWACPFLLA IWTLAGRFIL SLPWYATVLP 

       190        200        210        220        230        240 
MFLPTFYLWA VDEFALHRGT WSIGSGTKLD FCLFGKLDIE EATFFLVTNM LIVGGMAAFD 

       250        260        270        280        290        300 
QYLAVIYAFP TLFPKVNRYP TTHMLLQSRL INTSRYDLER IEGLREAVER LRLKSRSFYL 

       310        320        330        340        350        360 
ANSLFSGRLR IDLILLYSFC RLADDLVDDA KSRREVLSWT AKLNHFLDLH YKDADATEDP 

       370        380        390        400        410        420 
KKKAERIDAY IKTAFPPCAY QALHLLPTHI LPPKPLYDLI KGFEMDSQFT FHGTSDSTDL 

       430        440        450        460        470        480 
QYPIADDKDL ENYAIYVAGT VGELCIALII YHCLPDMSDT QKRELETAAC RMGIALQYVN 

       490        500        510        520        530        540 
IARDIVVDAR IGRVYLPTTW LKKEGLTHKM VLENPEGPEV IERMRRRLLE NAFELYGGAR 

       550        560        570        580        590        600 
PEMQRIPSEA RGPMIGAVEN YMAIGRVLRE RKEGTVFVRM EGRATVPKRR RLSTLLRALY 


EQ 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of al-2, the phytoene synthase gene of Neurospora crassa. Cloning, sequence analysis, and photoregulation."
Schmidhauser T.J., Lauter F.-R., Schumacher M., Zhou W., Russo V.E.A., Yanofsky C.
J. Biol. Chem. 269:12060-12066(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]"Mutants of the carotene cyclase domain of al-2 from Neurospora crassa."
Arrach N., Schmidhauser T.J., Avalos J.
Mol. Genet. Genomics 266:914-921(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF TRP-189 AND LYS-208.
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[5]"The biotechnological potential of the al-2 gene from Neurospora crassa for the production of monocyclic keto hydroxy carotenoids."
Sandmann G., Zhu C., Krubasik P., Fraser P.D.
Biochim. Biophys. Acta 1761:1085-1092(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L27652 Genomic DNA. Translation: AAA19428.1.
BX842641 Genomic DNA. Translation: CAE76609.1.
CM002236 Genomic DNA. Translation: EAA36489.3.
PIRA53583.
RefSeqXP_965725.2. XM_960632.2.
UniGeneNcr.25908.

3D structure databases

ProteinModelPortalP37295.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU00585.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000000733; EFNCRP00000000733; EFNCRG00000000733.
GeneID3881954.
KEGGncr:NCU00585.

Phylogenomic databases

eggNOGCOG1562.
KOK17841.
OrthoDBEOG7RBZJ2.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16129.
UniPathwayUPA00799; UER00773.
UPA00802.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
TIGRFAMsTIGR03462. CarR_dom_SF. 2 hits.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLCPS_NEUCR
AccessionPrimary (citable) accession number: P37295
Secondary accession number(s): Q7RVN3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways