ID   CRTB_SYNY3              Reviewed;         337 AA.
AC   P37294;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   27-MAR-2024, entry version 129.
DE   RecName: Full=15-cis-phytoene synthase {ECO:0000305};
DE            Short=PSase;
DE            EC=2.5.1.32 {ECO:0000269|PubMed:8018713};
GN   Name=crtB; Synonyms=pys; OrderedLocusNames=slr1255;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales; Merismopediaceae;
OC   Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=8018713; DOI=10.1016/0167-4781(94)90003-5;
RA   Martinez-Ferez I.M., Fernandez-Gonzalez B., Sandmann G., Vioque A.;
RT   "Cloning and expression in Escherichia coli of the gene coding for phytoene
RT   synthase from the cyanobacterium Synechocystis sp. PCC6803.";
RL   Biochim. Biophys. Acta 1218:145-152(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes the
CC       condensation of two molecules of geranylgeranyl diphosphate (GGPP) to
CC       give prephytoene diphosphate (PPPP) and the subsequent rearrangement of
CC       the cyclopropylcarbinyl intermediate to yield 15-cis-phytoene.
CC       {ECO:0000269|PubMed:8018713}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC         diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC         Evidence={ECO:0000269|PubMed:8018713};
CC   -!- COFACTOR:
CC       Name=ATP; Xref=ChEBI:CHEBI:30616; Evidence={ECO:0000250};
CC       Note=ATP is required for the transferase activity but it does not seem
CC       to be hydrolyzed during the reaction. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The enzyme produces 15-cis-phytoene. The conversion to all-
CC       trans-phytoene is due to photoisomerisation.
CC       {ECO:0000250|UniProtKB:D5KXJ0}.
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DR   EMBL; X69172; CAA48922.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA17848.1; -; Genomic_DNA.
DR   PIR; S45360; S45360.
DR   AlphaFoldDB; P37294; -.
DR   SMR; P37294; -.
DR   STRING; 1148.gene:10498716; -.
DR   PaxDb; 1148-1652930; -.
DR   EnsemblBacteria; BAA17848; BAA17848; BAA17848.
DR   KEGG; syn:slr1255; -.
DR   eggNOG; COG1562; Bacteria.
DR   InParanoid; P37294; -.
DR   PhylomeDB; P37294; -.
DR   UniPathway; UPA00799; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   PANTHER; PTHR31480; BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE; 1.
DR   PANTHER; PTHR31480:SF2; PHYTOENE SYNTHASE, CHLOROPLASTIC; 1.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDS00005; Isoprenoid_Synthase_Type_I; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; Terpenoid synthases; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Magnesium; Manganese; Metal-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..337
FT                   /note="15-cis-phytoene synthase"
FT                   /id="PRO_0000067438"
SQ   SEQUENCE   337 AA;  38707 MW;  860C059CFC92B7F8 CRC64;
     MANGQISPQR AVTKPQSWWL TSEPRPSLML QLPKPSPSAK PCASVEEAYE ICRQVTAQYA
     KTFYLGTLLM PPAKRRAIWA IYLWCRRTDE LVDGPQAATT TPETLDHWER RLEGIFAGQP
     QDDADVALVD TLETFPLDIQ PFRDMIAGQR MDLYRSRYQT FEELDLYCYR VAGTVGLMSS
     AVLGVDTGNG QAPWQPDAVY IPQEEAIALG VANQLTNILR DVGEDVERGR IYLPLEDLER
     FNYSEQDLLN GVNDDRWRSL MKFEIDRARH YFEDAERGIR ALNRDARWPV WTALMLYKGI
     LDVIEANNYN VFNRRAYVPT PKKLLYLPVA WLRAQVL
//