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P37294 (CRTB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
All-trans-phytoene synthase

Short name=PSase
EC=2.5.1.99
Gene names
Name:crtB
Synonyms:pys
Ordered Locus Names:slr1255
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield all-trans phytoene. Ref.1

Catalytic activity

2 geranylgeranyl diphosphate = all-trans-phytoene + 2 diphosphate. Ref.1

Cofactor

ATP. ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction By similarity.

Manganese or magnesium By similarity.

Pathway

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Sequence similarities

Belongs to the phytoene/squalene synthase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337All-trans-phytoene synthase
PRO_0000067438

Sequences

Sequence LengthMass (Da)Tools
P37294 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 860C059CFC92B7F8

FASTA33738,707
        10         20         30         40         50         60 
MANGQISPQR AVTKPQSWWL TSEPRPSLML QLPKPSPSAK PCASVEEAYE ICRQVTAQYA 

        70         80         90        100        110        120 
KTFYLGTLLM PPAKRRAIWA IYLWCRRTDE LVDGPQAATT TPETLDHWER RLEGIFAGQP 

       130        140        150        160        170        180 
QDDADVALVD TLETFPLDIQ PFRDMIAGQR MDLYRSRYQT FEELDLYCYR VAGTVGLMSS 

       190        200        210        220        230        240 
AVLGVDTGNG QAPWQPDAVY IPQEEAIALG VANQLTNILR DVGEDVERGR IYLPLEDLER 

       250        260        270        280        290        300 
FNYSEQDLLN GVNDDRWRSL MKFEIDRARH YFEDAERGIR ALNRDARWPV WTALMLYKGI 

       310        320        330 
LDVIEANNYN VFNRRAYVPT PKKLLYLPVA WLRAQVL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression in Escherichia coli of the gene coding for phytoene synthase from the cyanobacterium Synechocystis sp. PCC6803."
Martinez-Ferez I.M., Fernandez-Gonzalez B., Sandmann G., Vioque A.
Biochim. Biophys. Acta 1218:145-152(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69172 Genomic DNA. Translation: CAA48922.1.
BA000022 Genomic DNA. Translation: BAA17848.1.
PIRS45360.
RefSeqNP_441168.1. NC_000911.1.
YP_007451050.1. NC_020286.1.

3D structure databases

ProteinModelPortalP37294.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1148.slr1255.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17848; BAA17848; BAA17848.
GeneID14616705.
954504.
KEGGsyn:slr1255.
syz:MYO_112830.
PATRIC23839649. VBISynSp132158_1387.

Phylogenomic databases

eggNOGCOG1562.
HOGENOMHOG000220848.
KOK02291.
OMAKRRAIWA.
OrthoDBEOG63FW00.
PhylomeDBP37294.
ProtClustDBCLSK893015.

Enzyme and pathway databases

BioCycSSP1148:GJOT-1287-MONOMER.
UniPathwayUPA00799; UER00773.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRTB_SYNY3
AccessionPrimary (citable) accession number: P37294
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways