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Protein

All-trans-phytoene synthase

Gene

crtB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield all-trans phytoene.1 Publication

Catalytic activityi

2 geranylgeranyl diphosphate = all-trans-phytoene + 2 diphosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • ATPBy similarityNote: ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction.By similarity
  • Mn2+By similarity, Mg2+By similarity

Pathwayi: phytoene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. All-trans-phytoene synthase (crtB)
This subpathway is part of the pathway phytoene biosynthesis, which is itself part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate, the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • carotenoid biosynthetic process Source: UniProtKB
  • ergosterol biosynthetic process Source: InterPro

Keywordsi

Molecular functionTransferase
Biological processCarotenoid biosynthesis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.

Names & Taxonomyi

Protein namesi
Recommended name:
All-trans-phytoene synthase (EC:2.5.1.99)
Short name:
PSase
Gene namesi
Name:crtB
Synonyms:pys
Ordered Locus Names:slr1255
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000674381 – 337All-trans-phytoene synthaseAdd BLAST337

Proteomic databases

PRIDEiP37294.

Interactioni

Protein-protein interaction databases

STRINGi1148.SYNGTS_1272.

Structurei

3D structure databases

ProteinModelPortaliP37294.
SMRiP37294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000220848.
InParanoidiP37294.
KOiK02291.
OMAiMIAGQRM.
PhylomeDBiP37294.

Family and domain databases

CDDicd00683. Trans_IPPS_HH. 1 hit.
Gene3Di1.10.600.10. 1 hit.
InterProiView protein in InterPro
IPR008949. Isoprenoid_synthase_dom_sf.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR033904. Trans_IPPS_HH.
PfamiView protein in Pfam
PF00494. SQS_PSY. 1 hit.
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiView protein in PROSITE
PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P37294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANGQISPQR AVTKPQSWWL TSEPRPSLML QLPKPSPSAK PCASVEEAYE
60 70 80 90 100
ICRQVTAQYA KTFYLGTLLM PPAKRRAIWA IYLWCRRTDE LVDGPQAATT
110 120 130 140 150
TPETLDHWER RLEGIFAGQP QDDADVALVD TLETFPLDIQ PFRDMIAGQR
160 170 180 190 200
MDLYRSRYQT FEELDLYCYR VAGTVGLMSS AVLGVDTGNG QAPWQPDAVY
210 220 230 240 250
IPQEEAIALG VANQLTNILR DVGEDVERGR IYLPLEDLER FNYSEQDLLN
260 270 280 290 300
GVNDDRWRSL MKFEIDRARH YFEDAERGIR ALNRDARWPV WTALMLYKGI
310 320 330
LDVIEANNYN VFNRRAYVPT PKKLLYLPVA WLRAQVL
Length:337
Mass (Da):38,707
Last modified:October 1, 1994 - v1
Checksum:i860C059CFC92B7F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69172 Genomic DNA. Translation: CAA48922.1.
BA000022 Genomic DNA. Translation: BAA17848.1.
PIRiS45360.

Genome annotation databases

EnsemblBacteriaiBAA17848; BAA17848; BAA17848.
KEGGisyn:slr1255.

Similar proteinsi

Entry informationi

Entry nameiCRTB_SYNY3
AccessioniPrimary (citable) accession number: P37294
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 22, 2017
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names