P37294 (CRTB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytoene synthase
EC=2.5.1.32

Gene names

Name:	crtB
Synonyms:	pys
Ordered Locus Names:	slr1255

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene.
Catalytic activity	2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Phytoene synthase		PRO_0000067438

Sequences

	Sequence		Length	Mass (Da)
	P37294 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 860C059CFC92B7F8	FASTA	337	38,707
	10 20 30 40 50 60 MANGQISPQR AVTKPQSWWL TSEPRPSLML QLPKPSPSAK PCASVEEAYE ICRQVTAQYA 70 80 90 100 110 120 KTFYLGTLLM PPAKRRAIWA IYLWCRRTDE LVDGPQAATT TPETLDHWER RLEGIFAGQP 130 140 150 160 170 180 QDDADVALVD TLETFPLDIQ PFRDMIAGQR MDLYRSRYQT FEELDLYCYR VAGTVGLMSS 190 200 210 220 230 240 AVLGVDTGNG QAPWQPDAVY IPQEEAIALG VANQLTNILR DVGEDVERGR IYLPLEDLER 250 260 270 280 290 300 FNYSEQDLLN GVNDDRWRSL MKFEIDRARH YFEDAERGIR ALNRDARWPV WTALMLYKGI 310 320 330 LDVIEANNYN VFNRRAYVPT PKKLLYLPVA WLRAQVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression in Escherichia coli of the gene coding for phytoene synthase from the cyanobacterium Synechocystis sp. PCC6803." Martinez-Ferez I.M., Fernandez-Gonzalez B., Sandmann G., Vioque A. Biochim. Biophys. Acta 1218:145-152(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69172 Genomic DNA. Translation: CAA48922.1. BA000022 Genomic DNA. Translation: BAA17848.1.
PIR	S45360.
RefSeq	NP_441168.1. NC_000911.1. YP_007451050.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P37294.
ModBase	Search...
Protein-protein interaction databases
STRING	1148.slr1255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17848; BAA17848; BAA17848.
GeneID	14616705. 954504.
KEGG	syn:slr1255.
PATRIC	23839649. VBISynSp132158_1387.
Phylogenomic databases
eggNOG	COG1562.
HOGENOM	HOG000220848.
KO	K02291.
OMA	ELKLYCY.
ProtClustDB	CLSK893015.
Enzyme and pathway databases
BioCyc	SSP1148:GJOT-1287-MONOMER.
UniPathway	UPA00799; UER00773.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTB_SYNY3

Accession

Primary (citable) accession number: P37294

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 29, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program