Reviewed, UniProtKB/Swiss-Prot P37293 (NAT2_YEAST)

Names and origin

Protein names

Recommended name:
    N-terminal acetyltransferase 2
    EC=2.3.1.88
Alternative name(s):
    Amino-terminal, alpha-amino, acetyltransferase 2

Gene names

Name:	NAT2
Ordered Locus Names:	YGR147C
ORF Names:	G6630

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	N-terminal acetylation of proteins. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover. NAT2 acts on proteins with a N-terminal methionine.
Catalytic activity	Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.
Subunit structure	Heterooligomeric Potential.
Subcellular location	Cytoplasm Probable.
Miscellaneous	Present with 1240 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal peptidyl-methionine acetylation Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	peptide alpha-N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	N-terminal acetyltransferase 2		PRO_0000096740
Experimental info
	Sequence conflict	61	1	Q → K Ref.1
	Sequence conflict	63	1	R → S Ref.1
	Sequence conflict	218	1	V → L in AAA34812. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37293-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: C259310FD2D2B080	FASTA	288	32,804
	10 20 30 40 50 60 MMVPRISASP VFKRIFLRWG FVTLPIQKTV SHTLRRDFSA PCRSMVKCLL LRPGISVHSA 70 80 90 100 110 120 QDRKFYSTEE KSSQFDENKS KSNNGKKNEP HGIKGLMAKY GYSALIVYIL LTCVDLPLCF 130 140 150 160 170 180 LGVHSLGEEK IKIYLNRGKQ LIGMGEPDES KVIQDVRRKQ AHREAVQAEN ADKVEDASRK 190 200 210 220 230 240 TFNERWQEMK DSTLLAELLI AYGIHKSLII VRVPLTAVLT PSFVKLLQRF GIDLMKKQKK 250 260 270 280 VFQTMASGAK IRYKGNNPSD FIKNEGTALD ITKRKPRTKG QKWFDGLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAT2, an essential gene encoding methionine N alpha-acetyltransferase in the yeast Saccharomyces cerevisiae." Sherman F., Kulkarni M.S. J. Biol. Chem. 269:13141-13147(1994) [PubMed: 8175741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames." Skala J., Nawrocki A., Goffeau A. Yeast 11:1421-1427(1995) [PubMed: 8585325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L25608 Genomic DNA. Translation: AAA34812.1. X85807 Genomic DNA. Translation: CAA59805.1. Z72932 Genomic DNA. Translation: CAA97161.1.
PIR	S60438.
RefSeq	NP_011663.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P37293.
Genome annotation databases
Ensembl	YGR147C; YGR147C; YGR147C; Saccharomyces cerevisiae. [Genome view]
GeneID	853050.
GenomeReviews	Gene locus YGR147C in contig Y13135_GR.
KEGG	sce:YGR147C.
NMPDR	fig|4932.3.peg.2783.
Organism-specific databases
CYGD	YGR147c.
SGD	S000003379. NAT2.
Phylogenomic databases
HOGENOM	P37293.
OMA	RNEIREK.
Enzyme and pathway databases
BRENDA	2.3.1.88. 250.
Gene expression databases
ArrayExpress	P37293.
Genevestigator	P37293.
GermOnline	YGR147C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	972969.

Entry information

Entry name

NAT2_YEAST

Accession

Primary (citable) accession number: P37293

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)