P37293 (NAT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-terminal acetyltransferase 2
EC=2.3.1.88

Alternative name(s):
Amino-terminal, alpha-amino, acetyltransferase 2

Gene names

Name:	NAT2
Ordered Locus Names:	YGR147C
ORF Names:	G6630

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	N-terminal acetylation of proteins. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover. NAT2 acts on proteins with a N-terminal methionine.
Catalytic activity	Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.
Subunit structure	Heterooligomeric Potential.
Subcellular location	Cytoplasm Probable.
Miscellaneous	Present with 1240 molecules/cell in log phase SD medium. Ref.5

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal peptidyl-methionine acetylation Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	peptide alpha-N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	N-terminal acetyltransferase 2		PRO_0000096740
Experimental info
	Sequence conflict	61	1	Q → K Ref.1
	Sequence conflict	63	1	R → S Ref.1
	Sequence conflict	218	1	V → L in AAA34812. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37293 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: C259310FD2D2B080	FASTA	288	32,804
	10 20 30 40 50 60 MMVPRISASP VFKRIFLRWG FVTLPIQKTV SHTLRRDFSA PCRSMVKCLL LRPGISVHSA 70 80 90 100 110 120 QDRKFYSTEE KSSQFDENKS KSNNGKKNEP HGIKGLMAKY GYSALIVYIL LTCVDLPLCF 130 140 150 160 170 180 LGVHSLGEEK IKIYLNRGKQ LIGMGEPDES KVIQDVRRKQ AHREAVQAEN ADKVEDASRK 190 200 210 220 230 240 TFNERWQEMK DSTLLAELLI AYGIHKSLII VRVPLTAVLT PSFVKLLQRF GIDLMKKQKK 250 260 270 280 VFQTMASGAK IRYKGNNPSD FIKNEGTALD ITKRKPRTKG QKWFDGLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAT2, an essential gene encoding methionine N alpha-acetyltransferase in the yeast Saccharomyces cerevisiae." Sherman F., Kulkarni M.S. J. Biol. Chem. 269:13141-13147(1994) [PubMed: 8175741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames." Skala J., Nawrocki A., Goffeau A. Yeast 11:1421-1427(1995) [PubMed: 8585325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L25608 Genomic DNA. Translation: AAA34812.1. X85807 Genomic DNA. Translation: CAA59805.1. Z72932 Genomic DNA. Translation: CAA97161.1. BK006941 Genomic DNA. Translation: DAA08239.1.
PIR	S60438.
RefSeq	NP_011663.1. NM_001181276.1.
3D structure databases
ProteinModelPortal	P37293.
ModBase	Search...
Protein-protein interaction databases
STRING	P37293.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR147C; YGR147C; YGR147C.
GeneID	853050.
KEGG	sce:YGR147C.
NMPDR	fig|4932.3.peg.2783.
Organism-specific databases
CYGD	YGR147c.
SGD	S000003379. NAT2.
Phylogenomic databases
eggNOG	fuNOG11669.
GeneTree	EFGT00050000005538.
OMA	GEPDESK.
OrthoDB	EOG4897XC.
Gene expression databases
ArrayExpress	P37293.
Genevestigator	P37293.
GermOnline	YGR147C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009688. DUF1279. [Graphical view]
KO	K00670.
Pfam	PF06916. DUF1279. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972969.

Entry information

Entry name

NAT2_YEAST

Accession

Primary (citable) accession number: P37293
Secondary accession number(s): D6VUS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program