Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P37292

- GLYM_YEAST

UniProt

P37292 - GLYM_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Serine hydroxymethyltransferase, mitochondrial

Gene

SHM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Interconversion of serine and glycine.

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.

Cofactori

Pathwayi

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: SGD
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glycine metabolic process Source: InterPro
  2. L-serine metabolic process Source: InterPro
  3. one-carbon metabolic process Source: SGD
  4. serine family amino acid biosynthetic process Source: SGD
  5. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YBR263W-MONOMER.
YEAST:YBR263W-MONOMER.
ReactomeiREACT_234771. Carnitine synthesis.
REACT_261783. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferase, mitochondrial (EC:2.1.2.1)
Short name:
SHMT
Alternative name(s):
Glycine hydroxymethyltransferase
Serine methylase
Gene namesi
Name:SHM1
Synonyms:SHMT1
Ordered Locus Names:YBR263W
ORF Names:YBR1732
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR263w.
SGDiS000000467. SHM1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2020MitochondrionSequence AnalysisAdd
BLAST
Chaini21 – 490470Serine hydroxymethyltransferase, mitochondrialPRO_0000032568Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei265 – 2651N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiP37292.
PaxDbiP37292.
PeptideAtlasiP37292.
PRIDEiP37292.

Expressioni

Gene expression databases

GenevestigatoriP37292.

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with NAP1.By similarity1 Publication

Protein-protein interaction databases

BioGridi32958. 35 interactions.
DIPiDIP-4952N.
IntActiP37292. 4 interactions.
MINTiMINT-548725.
STRINGi4932.YBR263W.

Structurei

3D structure databases

ProteinModelPortaliP37292.
SMRiP37292. Positions 23-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SHMT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0112.
GeneTreeiENSGT00390000002762.
HOGENOMiHOG000239405.
InParanoidiP37292.
KOiK00600.
OMAiCGAYLMS.
OrthoDBiEOG7PVWZT.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37292-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFPRASALAK CMATVHRRGL LTSGAQSLVS KPVSEGDPEM FDILQQERHR
60 70 80 90 100
QKHSITLIPS ENFTSKAVMD LLGSELQNKY SEGYPGERYY GGNEIIDKSE
110 120 130 140 150
SLCQARALEL YGLDPAKWGV NVQPLSGAPA NLYVYSAIMN VGERLMGLDL
160 170 180 190 200
PDGGHLSHGY QLKSGTPISF ISKYFQSMPY HVDHTTGLID YDNLQVLAKA
210 220 230 240 250
FRPKVIVAGT SAYSRLIDYA RFKEISQGCG AYLMSDMAHI SGLVAANVVP
260 270 280 290 300
SPFEHSDIVT TTTHKSLRGP RGAMIFFRKG IKSVTKKGKE IPYELEKKIN
310 320 330 340 350
FSVFPGHQGG PHNHTIGAMA VALKQAMSPE FKEYQQKIVD NSKWFAQELT
360 370 380 390 400
KMGYKLVSGG TDNHLIVIDL SGTQVDGARV ETILSALNIA ANKNTIPGDK
410 420 430 440 450
SALFPSGLRI GTPAMTTRGF GREEFSQVAK YIDSAVKLAE NLKTLEPTTK
460 470 480 490
LDARSRLNEF KKLCNESSEV AALSGEISKW VGQYPVPGDI
Length:490
Mass (Da):53,686
Last modified:December 6, 2005 - v2
Checksum:i8DECF1ECD6349450
GO

Sequence cautioni

The sequence CAA49927.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA85226.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721L → S in AAA21024. (PubMed:8132653)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22528 Genomic DNA. Translation: AAA21024.1.
X70529 Genomic DNA. Translation: CAA49927.1. Different initiation.
Z36131 Genomic DNA. Translation: CAA85226.1. Different initiation.
BK006936 Genomic DNA. Translation: DAA07380.1.
PIRiS29348.
RefSeqiNP_009822.4. NM_001178611.3.

Genome annotation databases

EnsemblFungiiYBR263W; YBR263W; YBR263W.
GeneIDi852565.
KEGGisce:YBR263W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22528 Genomic DNA. Translation: AAA21024.1 .
X70529 Genomic DNA. Translation: CAA49927.1 . Different initiation.
Z36131 Genomic DNA. Translation: CAA85226.1 . Different initiation.
BK006936 Genomic DNA. Translation: DAA07380.1 .
PIRi S29348.
RefSeqi NP_009822.4. NM_001178611.3.

3D structure databases

ProteinModelPortali P37292.
SMRi P37292. Positions 23-488.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32958. 35 interactions.
DIPi DIP-4952N.
IntActi P37292. 4 interactions.
MINTi MINT-548725.
STRINGi 4932.YBR263W.

Proteomic databases

MaxQBi P37292.
PaxDbi P37292.
PeptideAtlasi P37292.
PRIDEi P37292.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR263W ; YBR263W ; YBR263W .
GeneIDi 852565.
KEGGi sce:YBR263W.

Organism-specific databases

CYGDi YBR263w.
SGDi S000000467. SHM1.

Phylogenomic databases

eggNOGi COG0112.
GeneTreei ENSGT00390000002762.
HOGENOMi HOG000239405.
InParanoidi P37292.
KOi K00600.
OMAi CGAYLMS.
OrthoDBi EOG7PVWZT.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci MetaCyc:YBR263W-MONOMER.
YEAST:YBR263W-MONOMER.
Reactomei REACT_234771. Carnitine synthesis.
REACT_261783. Metabolism of folate and pterines.

Miscellaneous databases

NextBioi 971682.

Gene expression databases

Genevestigatori P37292.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00051. SHMT.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view ]
PANTHERi PTHR11680. PTHR11680. 1 hit.
Pfami PF00464. SHMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000412. SHMT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00096. SHMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and molecular characterization of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine."
    McNeil J.B., McIntosh E.M., Taylor B.V., Zhang F.-R., Tang S., Bognar A.L.
    J. Biol. Chem. 269:9155-9165(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 208353 / W303-1A.
  2. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Doignon F., Biteau N., Crouzet M., Aigle M.
    Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
    Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
    Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGLYM_YEAST
AccessioniPrimary (citable) accession number: P37292
Secondary accession number(s): D6VQR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 6, 2005
Last modified: November 26, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two forms of the enzymes: a cytosolic one and a mitochondrial one.
Present with 17700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3