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P37291 (GLYC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine hydroxymethyltransferase, cytosolic

Short name=SHMT
EC=2.1.2.1
Alternative name(s):
Glycine hydroxymethyltransferase
Serine methylase
Gene names
Name:SHM2
Synonyms:SHMT2
Ordered Locus Names:YLR058C
ORF Names:L2156
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interconversion of serine and glycine. HAMAP-Rule MF_00051

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP-Rule MF_00051

Cofactor

Pyridoxal phosphate.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_00051

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00051

Subcellular location

Cytoplasm HAMAP-Rule MF_00051.

Miscellaneous

In eukaryotes there are two forms of the enzymes: a cytosolic one and a mitochondrial one.

Present with 67600 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Serine hydroxymethyltransferase, cytosolic HAMAP-Rule MF_00051
PRO_0000113516

Amino acid modifications

Modified residue201Phosphothreonine Ref.7
Modified residue261Phosphoserine Ref.6 Ref.7
Modified residue2481N6-(pyridoxal phosphate)lysine By similarity
Modified residue4291Phosphoserine Ref.6 Ref.7

Experimental info

Sequence conflict4291S → K in AAA21023. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P37291 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 4A79F79ED30A5965

FASTA46952,218
        10         20         30         40         50         60 
MPYTLSDAHH KLITSHLVDT DPEVDSIIKD EIERQKHSID LIASENFTST SVFDALGTPL 

        70         80         90        100        110        120 
SNKYSEGYPG ARYYGGNEHI DRMEILCQQR ALKAFHVTPD KWGVNVQTLS GSPANLQVYQ 

       130        140        150        160        170        180 
AIMKPHERLM GLYLPDGGHL SHGYATENRK ISAVSTYFES FPYRVNPETG IIDYDTLEKN 

       190        200        210        220        230        240 
AILYRPKVLV AGTSAYCRLI DYKRMREIAD KCGAYLMVDM AHISGLIAAG VIPSPFEYAD 

       250        260        270        280        290        300 
IVTTTTHKSL RGPRGAMIFF RRGVRSINPK TGKEVLYDLE NPINFSVFPG HQGGPHNHTI 

       310        320        330        340        350        360 
AALATALKQA ATPEFKEYQT QVLKNAKALE SEFKNLGYRL VSNGTDSHMV LVSLREKGVD 

       370        380        390        400        410        420 
GARVEYICEK INIALNKNSI PGDKSALVPG GVRIGAPAMT TRGMGEEDFH RIVQYINKAV 

       430        440        450        460 
EFAQQVQQSL PKDACRLKDF KAKVDEGSDV LNTWKKEIYD WAGEYPLAV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and molecular characterization of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine."
McNeil J.B., McIntosh E.M., Taylor B.V., Zhang F.-R., Tang S., Bognar A.L.
J. Biol. Chem. 269:9155-9165(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 201238 / W303-1B.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3-10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI > 7) proteins."
Norbeck J., Blomberg A.
Yeast 13:1519-1534(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 151-161 AND 274-281.
Strain: ATCC 44827 / SKQ2N.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-26 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L22529 Genomic DNA. Translation: AAA21023.1.
X94607 Genomic DNA. Translation: CAA64305.1.
Z73230 Genomic DNA. Translation: CAA97588.1.
BK006945 Genomic DNA. Translation: DAA09376.1.
PIRS61632.
RefSeqNP_013159.1. NM_001181945.1.

3D structure databases

ProteinModelPortalP37291.
SMRP37291. Positions 4-468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31333. 65 interactions.
DIPDIP-2602N.
IntActP37291. 16 interactions.
MINTMINT-423439.
STRING4932.YLR058C.

Proteomic databases

MaxQBP37291.
PaxDbP37291.
PeptideAtlasP37291.
PRIDEP37291.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR058C; YLR058C; YLR058C.
GeneID850747.
KEGGsce:YLR058C.

Organism-specific databases

CYGDYLR058c.
SGDS000004048. SHM2.

Phylogenomic databases

eggNOGCOG0112.
GeneTreeENSGT00390000002762.
HOGENOMHOG000239405.
KOK00600.
OMANQHIDEI.
OrthoDBEOG7PVWZT.

Enzyme and pathway databases

BioCycMetaCyc:YLR058C-MONOMER.
YEAST:YLR058C-MONOMER.
UniPathwayUPA00193.

Gene expression databases

GenevestigatorP37291.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00051. SHMT.
InterProIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERPTHR11680. PTHR11680. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966873.
PROP37291.

Entry information

Entry nameGLYC_YEAST
AccessionPrimary (citable) accession number: P37291
Secondary accession number(s): D6VY60
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways