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P37291

- GLYC_YEAST

UniProt

P37291 - GLYC_YEAST

Protein

Serine hydroxymethyltransferase, cytosolic

Gene

SHM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Interconversion of serine and glycine.

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: SGD
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. glycine metabolic process Source: InterPro
    2. L-serine metabolic process Source: InterPro
    3. one-carbon metabolic process Source: SGD
    4. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:YLR058C-MONOMER.
    YEAST:YLR058C-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferase, cytosolic (EC:2.1.2.1)
    Short name:
    SHMT
    Alternative name(s):
    Glycine hydroxymethyltransferase
    Serine methylase
    Gene namesi
    Name:SHM2
    Synonyms:SHMT2
    Ordered Locus Names:YLR058C
    ORF Names:L2156
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR058c.
    SGDiS000004048. SHM2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Serine hydroxymethyltransferase, cytosolicPRO_0000113516Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei20 – 201Phosphothreonine1 Publication
    Modified residuei26 – 261Phosphoserine2 Publications
    Modified residuei248 – 2481N6-(pyridoxal phosphate)lysineBy similarity
    Modified residuei429 – 4291Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP37291.
    PaxDbiP37291.
    PeptideAtlasiP37291.
    PRIDEiP37291.

    Expressioni

    Gene expression databases

    GenevestigatoriP37291.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi31333. 65 interactions.
    DIPiDIP-2602N.
    IntActiP37291. 16 interactions.
    MINTiMINT-423439.
    STRINGi4932.YLR058C.

    Structurei

    3D structure databases

    ProteinModelPortaliP37291.
    SMRiP37291. Positions 4-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SHMT family.Curated

    Phylogenomic databases

    eggNOGiCOG0112.
    GeneTreeiENSGT00390000002762.
    HOGENOMiHOG000239405.
    KOiK00600.
    OMAiNQHIDEI.
    OrthoDBiEOG7PVWZT.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P37291-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPYTLSDAHH KLITSHLVDT DPEVDSIIKD EIERQKHSID LIASENFTST    50
    SVFDALGTPL SNKYSEGYPG ARYYGGNEHI DRMEILCQQR ALKAFHVTPD 100
    KWGVNVQTLS GSPANLQVYQ AIMKPHERLM GLYLPDGGHL SHGYATENRK 150
    ISAVSTYFES FPYRVNPETG IIDYDTLEKN AILYRPKVLV AGTSAYCRLI 200
    DYKRMREIAD KCGAYLMVDM AHISGLIAAG VIPSPFEYAD IVTTTTHKSL 250
    RGPRGAMIFF RRGVRSINPK TGKEVLYDLE NPINFSVFPG HQGGPHNHTI 300
    AALATALKQA ATPEFKEYQT QVLKNAKALE SEFKNLGYRL VSNGTDSHMV 350
    LVSLREKGVD GARVEYICEK INIALNKNSI PGDKSALVPG GVRIGAPAMT 400
    TRGMGEEDFH RIVQYINKAV EFAQQVQQSL PKDACRLKDF KAKVDEGSDV 450
    LNTWKKEIYD WAGEYPLAV 469
    Length:469
    Mass (Da):52,218
    Last modified:October 1, 1996 - v2
    Checksum:i4A79F79ED30A5965
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti429 – 4291S → K in AAA21023. (PubMed:8132653)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22529 Genomic DNA. Translation: AAA21023.1.
    X94607 Genomic DNA. Translation: CAA64305.1.
    Z73230 Genomic DNA. Translation: CAA97588.1.
    BK006945 Genomic DNA. Translation: DAA09376.1.
    PIRiS61632.
    RefSeqiNP_013159.1. NM_001181945.1.

    Genome annotation databases

    EnsemblFungiiYLR058C; YLR058C; YLR058C.
    GeneIDi850747.
    KEGGisce:YLR058C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22529 Genomic DNA. Translation: AAA21023.1 .
    X94607 Genomic DNA. Translation: CAA64305.1 .
    Z73230 Genomic DNA. Translation: CAA97588.1 .
    BK006945 Genomic DNA. Translation: DAA09376.1 .
    PIRi S61632.
    RefSeqi NP_013159.1. NM_001181945.1.

    3D structure databases

    ProteinModelPortali P37291.
    SMRi P37291. Positions 4-468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31333. 65 interactions.
    DIPi DIP-2602N.
    IntActi P37291. 16 interactions.
    MINTi MINT-423439.
    STRINGi 4932.YLR058C.

    Proteomic databases

    MaxQBi P37291.
    PaxDbi P37291.
    PeptideAtlasi P37291.
    PRIDEi P37291.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR058C ; YLR058C ; YLR058C .
    GeneIDi 850747.
    KEGGi sce:YLR058C.

    Organism-specific databases

    CYGDi YLR058c.
    SGDi S000004048. SHM2.

    Phylogenomic databases

    eggNOGi COG0112.
    GeneTreei ENSGT00390000002762.
    HOGENOMi HOG000239405.
    KOi K00600.
    OMAi NQHIDEI.
    OrthoDBi EOG7PVWZT.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci MetaCyc:YLR058C-MONOMER.
    YEAST:YLR058C-MONOMER.

    Miscellaneous databases

    NextBioi 966873.
    PROi P37291.

    Gene expression databases

    Genevestigatori P37291.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and molecular characterization of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine."
      McNeil J.B., McIntosh E.M., Taylor B.V., Zhang F.-R., Tang S., Bognar A.L.
      J. Biol. Chem. 269:9155-9165(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 201238 / W303-1B.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3-10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI > 7) proteins."
      Norbeck J., Blomberg A.
      Yeast 13:1519-1534(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 151-161 AND 274-281.
      Strain: ATCC 44827 / SKQ2N.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-26 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLYC_YEAST
    AccessioniPrimary (citable) accession number: P37291
    Secondary accession number(s): D6VY60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are two forms of the enzymes: a cytosolic one and a mitochondrial one.
    Present with 67600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3