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Protein

Serine hydroxymethyltransferase, cytosolic

Gene

SHM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interconversion of serine and glycine.

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.

Cofactori

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

GO - Molecular functioni

  • glycine hydroxymethyltransferase activity Source: SGD
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • glycine metabolic process Source: InterPro
  • L-serine metabolic process Source: InterPro
  • one-carbon metabolic process Source: SGD
  • tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YLR058C-MONOMER.
YEAST:YLR058C-MONOMER.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.
R-SCE-71262. Carnitine synthesis.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferase, cytosolic (EC:2.1.2.1)
Short name:
SHMT
Alternative name(s):
Glycine hydroxymethyltransferase
Serine methylase
Gene namesi
Name:SHM2
Synonyms:SHMT2
Ordered Locus Names:YLR058C
ORF Names:L2156
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR058C.
SGDiS000004048. SHM2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001135161 – 469Serine hydroxymethyltransferase, cytosolicAdd BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphothreonineCombined sources1
Modified residuei26PhosphoserineCombined sources1
Modified residuei248N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei429PhosphoserineCombined sources1
Cross-linki456Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP37291.
PRIDEiP37291.

PTM databases

iPTMnetiP37291.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi31333. 71 interactors.
DIPiDIP-2602N.
IntActiP37291. 17 interactors.
MINTiMINT-423439.

Structurei

3D structure databases

ProteinModelPortaliP37291.
SMRiP37291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SHMT family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002762.
HOGENOMiHOG000239405.
InParanoidiP37291.
KOiK00600.
OMAiASIMKDE.
OrthoDBiEOG092C2GWL.

Family and domain databases

CDDicd00378. SHMT. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT. 1 hit.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYTLSDAHH KLITSHLVDT DPEVDSIIKD EIERQKHSID LIASENFTST
60 70 80 90 100
SVFDALGTPL SNKYSEGYPG ARYYGGNEHI DRMEILCQQR ALKAFHVTPD
110 120 130 140 150
KWGVNVQTLS GSPANLQVYQ AIMKPHERLM GLYLPDGGHL SHGYATENRK
160 170 180 190 200
ISAVSTYFES FPYRVNPETG IIDYDTLEKN AILYRPKVLV AGTSAYCRLI
210 220 230 240 250
DYKRMREIAD KCGAYLMVDM AHISGLIAAG VIPSPFEYAD IVTTTTHKSL
260 270 280 290 300
RGPRGAMIFF RRGVRSINPK TGKEVLYDLE NPINFSVFPG HQGGPHNHTI
310 320 330 340 350
AALATALKQA ATPEFKEYQT QVLKNAKALE SEFKNLGYRL VSNGTDSHMV
360 370 380 390 400
LVSLREKGVD GARVEYICEK INIALNKNSI PGDKSALVPG GVRIGAPAMT
410 420 430 440 450
TRGMGEEDFH RIVQYINKAV EFAQQVQQSL PKDACRLKDF KAKVDEGSDV
460
LNTWKKEIYD WAGEYPLAV
Length:469
Mass (Da):52,218
Last modified:October 1, 1996 - v2
Checksum:i4A79F79ED30A5965
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti429S → K in AAA21023 (PubMed:8132653).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22529 Genomic DNA. Translation: AAA21023.1.
X94607 Genomic DNA. Translation: CAA64305.1.
Z73230 Genomic DNA. Translation: CAA97588.1.
BK006945 Genomic DNA. Translation: DAA09376.1.
PIRiS61632.
RefSeqiNP_013159.1. NM_001181945.1.

Genome annotation databases

EnsemblFungiiYLR058C; YLR058C; YLR058C.
GeneIDi850747.
KEGGisce:YLR058C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22529 Genomic DNA. Translation: AAA21023.1.
X94607 Genomic DNA. Translation: CAA64305.1.
Z73230 Genomic DNA. Translation: CAA97588.1.
BK006945 Genomic DNA. Translation: DAA09376.1.
PIRiS61632.
RefSeqiNP_013159.1. NM_001181945.1.

3D structure databases

ProteinModelPortaliP37291.
SMRiP37291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31333. 71 interactors.
DIPiDIP-2602N.
IntActiP37291. 17 interactors.
MINTiMINT-423439.

PTM databases

iPTMnetiP37291.

Proteomic databases

MaxQBiP37291.
PRIDEiP37291.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR058C; YLR058C; YLR058C.
GeneIDi850747.
KEGGisce:YLR058C.

Organism-specific databases

EuPathDBiFungiDB:YLR058C.
SGDiS000004048. SHM2.

Phylogenomic databases

GeneTreeiENSGT00390000002762.
HOGENOMiHOG000239405.
InParanoidiP37291.
KOiK00600.
OMAiASIMKDE.
OrthoDBiEOG092C2GWL.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciMetaCyc:YLR058C-MONOMER.
YEAST:YLR058C-MONOMER.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.
R-SCE-71262. Carnitine synthesis.

Miscellaneous databases

PROiP37291.

Family and domain databases

CDDicd00378. SHMT. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT. 1 hit.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLYC_YEAST
AccessioniPrimary (citable) accession number: P37291
Secondary accession number(s): D6VY60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two forms of the enzymes: a cytosolic one and a mitochondrial one.
Present with 67600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.