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Protein

Kinesin light chain 1

Gene

Klc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.1 Publication

GO - Molecular functioni

  • microtubule motor activity Source: InterPro
  • tubulin binding Source: RGD

GO - Biological processi

  • intracellular protein transport Source: RGD
  • protein localization to synapse Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin light chain 1
Short name:
KLC 1
Gene namesi
Name:Klc1
Synonyms:Klc, Kns2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi621411. Klc1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • growth cone Source: UniProtKB
  • kinesin complex Source: RGD
  • membrane Source: BHF-UCL
  • microtubule Source: UniProtKB-KW
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Microtubule

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2176819.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Kinesin light chain 1PRO_0000215094Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei162 – 1621PhosphoserineCombined sources
Modified residuei449 – 4491PhosphotyrosineBy similarity
Modified residuei460 – 4601PhosphoserineBy similarity
Modified residuei521 – 5211PhosphoserineCombined sources
Modified residuei524 – 5241PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP37285.
PRIDEiP37285.

PTM databases

iPTMnetiP37285.
PhosphoSiteiP37285.
SwissPalmiP37285.

Expressioni

Tissue specificityi

Expressed in brain (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiP37285. baseline and differential.
GenevisibleiP37285. RN.

Interactioni

Subunit structurei

Oligomeric complex composed of two heavy chains and two light chains. Interacts with SPAG9. Interacts with ATCAY; may link mitochondria to KLC1 and regulate mitochondria localization into neuron projections. Interacts (via TPR repeats) with TOR1A; the interaction associates TOR1A with the kinesin oligomeric complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VACWR159P686194EBI-917396,EBI-7133540From a different organism.

GO - Molecular functioni

  • tubulin binding Source: RGD

Protein-protein interaction databases

BioGridi251058. 3 interactions.
DIPiDIP-36927N.
IntActiP37285. 2 interactions.
MINTiMINT-4591399.
STRINGi10116.ENSRNOP00000015935.

Structurei

3D structure databases

ProteinModelPortaliP37285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati213 – 24634TPR 1Add
BLAST
Repeati255 – 28834TPR 2Add
BLAST
Repeati297 – 33034TPR 3Add
BLAST
Repeati339 – 37234TPR 4Add
BLAST
Repeati381 – 41434TPR 5Add
BLAST
Repeati464 – 49734TPR 6Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili27 – 156130Add
BLAST

Sequence similaritiesi

Belongs to the kinesin light chain family.Curated
Contains 6 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1840. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00390000006393.
HOGENOMiHOG000261663.
HOVERGENiHBG006217.
InParanoidiP37285.
KOiK10407.
OMAiEREEHKY.
OrthoDBiEOG7TXKGD.
PhylomeDBiP37285.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR002151. Kinesin_light.
IPR015792. Kinesin_light_repeat.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13374. TPR_10. 2 hits.
[Graphical view]
PRINTSiPR00381. KINESINLIGHT.
SMARTiSM00028. TPR. 5 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS01160. KINESIN_LIGHT. 4 hits.
PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform C (identifier: P37285-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHDNMSTMVY MKEEKLEKLT QDEIISKTKQ VIQGLEALKN EHNSILQSLL
60 70 80 90 100
ETLKCLKKDD ESNLVEEKSS MIRKSLEMLE LGLSEAQVMM ALSNHLNAVE
110 120 130 140 150
SEKQKLRAQV RRLCQENQWL RDELANTQQK LQKSEQSVAQ LEEEKKHLEF
160 170 180 190 200
MNQLKKYDDD ISPSEDKDSD SSKEPLDDLF PNDEDDPGQG IQQQHSSAAA
210 220 230 240 250
AAQQGGYEIP ARLRTLHNLV IQYASQGRYE VAVPLCKQAL EDLEKTSGHD
260 270 280 290 300
HPDVATMLNI LALVYRDQNK YKDAANLLND ALAIREKTLG RDHPAVAATL
310 320 330 340 350
NNLAVLYGKR GKYKEAEPLC KRALEIREKV LGKDHPDVAK QLNNLALLCQ
360 370 380 390 400
NQGKYEEVEY YYQRALEIYQ TKLGPDDPNV AKTKNNLASC YLKQGKFKQA
410 420 430 440 450
ETLYKEILTR AHEREFGSVD DENKPIWMHA EEREECKGKQ KDGSSFGEYG
460 470 480 490 500
GWYKACKVDS PTVTTTLKNL GALYRRQGKF EAAETLEEAA LRSRKQGLDN
510 520 530 540 550
VHKQRVAEVL NDPENVEKRR SRESLNVDVV KYESGPDGGE EVSMSVEWNG
560
MRKMKLGLVK
Length:560
Mass (Da):63,745
Last modified:November 25, 2008 - v2
Checksum:i24D0C4CF9E304366
GO
Isoform A (identifier: P37285-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     542-560: VSMSVEWNGMRKMKLGLVK → A

Show »
Length:542
Mass (Da):61,640
Checksum:iC3C983FE82395AD9
GO
Isoform B (identifier: P37285-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     542-550: Missing.

Show »
Length:551
Mass (Da):62,755
Checksum:i3DFC93C236D877DA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei542 – 56019VSMSV…LGLVK → A in isoform A. 1 PublicationVSP_002872Add
BLAST
Alternative sequencei542 – 5509Missing in isoform B. 1 PublicationVSP_002871

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75146 mRNA. No translation available.
M75147 mRNA. No translation available.
M75148 mRNA. No translation available.
RefSeqiNP_001075442.1. NM_001081973.1. [P37285-3]
NP_001075443.1. NM_001081974.1. [P37285-1]
XP_006240688.1. XM_006240626.2. [P37285-1]
UniGeneiRn.106230.

Genome annotation databases

EnsembliENSRNOT00000065281; ENSRNOP00000060469; ENSRNOG00000011572. [P37285-2]
GeneIDi171041.
KEGGirno:171041.
UCSCiRGD:621411. rat. [P37285-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75146 mRNA. No translation available.
M75147 mRNA. No translation available.
M75148 mRNA. No translation available.
RefSeqiNP_001075442.1. NM_001081973.1. [P37285-3]
NP_001075443.1. NM_001081974.1. [P37285-1]
XP_006240688.1. XM_006240626.2. [P37285-1]
UniGeneiRn.106230.

3D structure databases

ProteinModelPortaliP37285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251058. 3 interactions.
DIPiDIP-36927N.
IntActiP37285. 2 interactions.
MINTiMINT-4591399.
STRINGi10116.ENSRNOP00000015935.

Chemistry

ChEMBLiCHEMBL2176819.

PTM databases

iPTMnetiP37285.
PhosphoSiteiP37285.
SwissPalmiP37285.

Proteomic databases

PaxDbiP37285.
PRIDEiP37285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000065281; ENSRNOP00000060469; ENSRNOG00000011572. [P37285-2]
GeneIDi171041.
KEGGirno:171041.
UCSCiRGD:621411. rat. [P37285-1]

Organism-specific databases

CTDi3831.
RGDi621411. Klc1.

Phylogenomic databases

eggNOGiKOG1840. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00390000006393.
HOGENOMiHOG000261663.
HOVERGENiHBG006217.
InParanoidiP37285.
KOiK10407.
OMAiEREEHKY.
OrthoDBiEOG7TXKGD.
PhylomeDBiP37285.

Miscellaneous databases

NextBioi621537.
PROiP37285.

Gene expression databases

ExpressionAtlasiP37285. baseline and differential.
GenevisibleiP37285. RN.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR002151. Kinesin_light.
IPR015792. Kinesin_light_repeat.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13374. TPR_10. 2 hits.
[Graphical view]
PRINTSiPR00381. KINESINLIGHT.
SMARTiSM00028. TPR. 5 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS01160. KINESIN_LIGHT. 4 hits.
PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular genetics of kinesin light chains: generation of isoforms by alternative splicing."
    Cyr J.L., Pfister K.K., Bloom G.S., Slaughter C.A., Brady S.T.
    Proc. Natl. Acad. Sci. U.S.A. 88:10114-10118(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C).
    Tissue: Brain.
  2. "The early onset dystonia protein torsinA interacts with kinesin light chain 1."
    Kamm C., Boston H., Hewett J., Wilbur J., Corey D.P., Hanson P.I., Ramesh V., Breakefield X.O.
    J. Biol. Chem. 279:19882-19892(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TOR1A, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "Cayman ataxia protein caytaxin is transported by kinesin along neurites through binding to kinesin light chains."
    Aoyama T., Hata S., Nakao T., Tanigawa Y., Oka C., Kawaichi M.
    J. Cell Sci. 122:4177-4185(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATCAY.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162; SER-521 AND SER-524, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKLC1_RAT
AccessioniPrimary (citable) accession number: P37285
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 25, 2008
Last modified: May 11, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-5 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.