P37271 (PSY_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytoene synthase, chloroplastic
EC=2.5.1.32

Gene names

Name:	PSY1
Synonyms:	PSY
Ordered Locus Names:	At5g17230
ORF Names:	MKP11.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene.
Catalytic activity	2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P37271-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 70	70	Chloroplast Potential
	Chain	71 – 422	352	Phytoene synthase, chloroplastic		PRO_0000029852
Experimental info
	Sequence conflict	60	1	R → M in AAM62787. Ref.6
	Sequence conflict	128	1	L → LV in AAA32836. Ref.1
	Sequence conflict	143	1	A → P in AAA32836. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: C44F0A512F2DD31E	FASTA	422	47,487
	10 20 30 40 50 60 MSSSVAVLWV ATSSLNPDPM NNCGLVRVLE SSRLFSPCQN QRLNKGKKKQ IPTWSSSFVR 70 80 90 100 110 120 NRSRRIGVVS SSLVASPSGE IALSSEEKVY NVVLKQAALV NKQLRSSSYD LDVKKPQDVV 130 140 150 160 170 180 LPGSLSLLGE AYDRCGEVCA EYAKTFYLGT LLMTPERRKA IWAIYVWCRR TDELVDGPNA 190 200 210 220 230 240 SHITPMALDR WEARLEDLFR GRPFDMLDAA LADTVARYPV DIQPFRDMIE GMRMDLKKSR 250 260 270 280 290 300 YQNFDDLYLY CYYVAGTVGL MSVPVMGIDP KSKATTESVY NAALALGIAN QLTNILRDVG 310 320 330 340 350 360 EDARRGRVYL PQDELAQAGL SDEDIFAGKV TDKWRNFMKM QLKRARMFFD EAEKGVTELS 370 380 390 400 410 420 AASRWPVWAS LLLYRRILDE IEANDYNNFT KRAYVGKVKK IAALPLAYAK SVLKTSSSRL SI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of an Arabidopsis cDNA for phytoene synthase." Scolnik P.A., Bartley G.E. Plant Physiol. 104:1471-1472(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence of the phytoene synthase gene of Arabidopsis." Castrignano F., Giuliano G. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L25812 mRNA. Translation: AAA32836.1. AF009954 Genomic DNA. Translation: AAB65697.1. AB005238 Genomic DNA. Translation: BAB10510.1. CP002688 Genomic DNA. Translation: AED92399.1. CP002688 Genomic DNA. Translation: AED92400.1. BT000450 mRNA. Translation: AAN17427.1. BT002084 mRNA. Translation: AAN72095.1. AY085565 mRNA. Translation: AAM62787.1.
IPI	IPI00527204.
RefSeq	NP_001031895.1. NM_001036818.2. NP_197225.1. NM_121729.2.
UniGene	At.23726.
3D structure databases
ProteinModelPortal	P37271.
SMR	P37271. Positions 132-389.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G17230.3-P.
Proteomic databases
PaxDb	P37271.
PRIDE	P37271.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G17230.1; AT5G17230.1; AT5G17230. AT5G17230.2; AT5G17230.2; AT5G17230.
GeneID	831587.
KEGG	ath:AT5G17230.
Organism-specific databases
TAIR	At5g17230.
Phylogenomic databases
eggNOG	COG1562.
HOGENOM	HOG000220848.
InParanoid	P37271.
KO	K02291.
PhylomeDB	P37271.
ProtClustDB	PLN02632.
Enzyme and pathway databases
BioCyc	ARA:AT5G17230-MONOMER. MetaCyc:AT5G17230-MONOMER.
UniPathway	UPA00799; UER00773.
Gene expression databases
ArrayExpress	P37271.
Genevestigator	P37271.
GermOnline	AT5G17230. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSY_ARATH

Accession

Primary (citable) accession number: P37271
Secondary accession number(s): O22375, Q8LE86

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 10, 2003
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program