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Protein

Phytoene synthase, chloroplastic

Gene

PSY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reaction from prephytoene diphosphate to phytoene.

Catalytic activityi

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathway:iphytoene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Phytoene synthase, chloroplastic (PSY1)
This subpathway is part of the pathway phytoene biosynthesis, which is itself part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate, the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis, Isoprene biosynthesis

Enzyme and pathway databases

BioCyciARA:AT5G17230-MONOMER.
ARA:GQT-752-MONOMER.
ARA:GQT-753-MONOMER.
MetaCyc:AT5G17230-MONOMER.
BRENDAi2.5.1.32. 399.
UniPathwayiUPA00799; UER00773.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene synthase, chloroplastic (EC:2.5.1.32)
Gene namesi
Name:PSY1
Synonyms:PSY
Ordered Locus Names:At5g17230
ORF Names:MKP11.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G17230.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7070ChloroplastSequence AnalysisAdd
BLAST
Chaini71 – 422352Phytoene synthase, chloroplasticPRO_0000029852Add
BLAST

Proteomic databases

PaxDbiP37271.
PRIDEiP37271.

Expressioni

Gene expression databases

ExpressionAtlasiP37271. baseline and differential.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G17230.3.

Structurei

3D structure databases

ProteinModelPortaliP37271.
SMRiP37271. Positions 132-389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000220848.
InParanoidiP37271.
KOiK02291.
PhylomeDBiP37271.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P37271-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSVAVLWV ATSSLNPDPM NNCGLVRVLE SSRLFSPCQN QRLNKGKKKQ
60 70 80 90 100
IPTWSSSFVR NRSRRIGVVS SSLVASPSGE IALSSEEKVY NVVLKQAALV
110 120 130 140 150
NKQLRSSSYD LDVKKPQDVV LPGSLSLLGE AYDRCGEVCA EYAKTFYLGT
160 170 180 190 200
LLMTPERRKA IWAIYVWCRR TDELVDGPNA SHITPMALDR WEARLEDLFR
210 220 230 240 250
GRPFDMLDAA LADTVARYPV DIQPFRDMIE GMRMDLKKSR YQNFDDLYLY
260 270 280 290 300
CYYVAGTVGL MSVPVMGIDP KSKATTESVY NAALALGIAN QLTNILRDVG
310 320 330 340 350
EDARRGRVYL PQDELAQAGL SDEDIFAGKV TDKWRNFMKM QLKRARMFFD
360 370 380 390 400
EAEKGVTELS AASRWPVWAS LLLYRRILDE IEANDYNNFT KRAYVGKVKK
410 420
IAALPLAYAK SVLKTSSSRL SI
Length:422
Mass (Da):47,487
Last modified:January 10, 2003 - v2
Checksum:iC44F0A512F2DD31E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601R → M in AAM62787 (Ref. 6) Curated
Sequence conflicti128 – 1281L → LV in AAA32836 (PubMed:8016277).Curated
Sequence conflicti143 – 1431A → P in AAA32836 (PubMed:8016277).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25812 mRNA. Translation: AAA32836.1.
AF009954 Genomic DNA. Translation: AAB65697.1.
AB005238 Genomic DNA. Translation: BAB10510.1.
CP002688 Genomic DNA. Translation: AED92399.1.
CP002688 Genomic DNA. Translation: AED92400.1.
BT000450 mRNA. Translation: AAN17427.1.
BT002084 mRNA. Translation: AAN72095.1.
AY085565 mRNA. Translation: AAM62787.1.
RefSeqiNP_001031895.1. NM_001036818.2. [P37271-1]
NP_197225.1. NM_121729.2. [P37271-1]
UniGeneiAt.23726.

Genome annotation databases

EnsemblPlantsiAT5G17230.1; AT5G17230.1; AT5G17230. [P37271-1]
AT5G17230.2; AT5G17230.2; AT5G17230. [P37271-1]
GeneIDi831587.
KEGGiath:AT5G17230.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25812 mRNA. Translation: AAA32836.1.
AF009954 Genomic DNA. Translation: AAB65697.1.
AB005238 Genomic DNA. Translation: BAB10510.1.
CP002688 Genomic DNA. Translation: AED92399.1.
CP002688 Genomic DNA. Translation: AED92400.1.
BT000450 mRNA. Translation: AAN17427.1.
BT002084 mRNA. Translation: AAN72095.1.
AY085565 mRNA. Translation: AAM62787.1.
RefSeqiNP_001031895.1. NM_001036818.2. [P37271-1]
NP_197225.1. NM_121729.2. [P37271-1]
UniGeneiAt.23726.

3D structure databases

ProteinModelPortaliP37271.
SMRiP37271. Positions 132-389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G17230.3.

Proteomic databases

PaxDbiP37271.
PRIDEiP37271.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G17230.1; AT5G17230.1; AT5G17230. [P37271-1]
AT5G17230.2; AT5G17230.2; AT5G17230. [P37271-1]
GeneIDi831587.
KEGGiath:AT5G17230.

Organism-specific databases

TAIRiAT5G17230.

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000220848.
InParanoidiP37271.
KOiK02291.
PhylomeDBiP37271.

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
BioCyciARA:AT5G17230-MONOMER.
ARA:GQT-752-MONOMER.
ARA:GQT-753-MONOMER.
MetaCyc:AT5G17230-MONOMER.
BRENDAi2.5.1.32. 399.

Miscellaneous databases

PROiP37271.

Gene expression databases

ExpressionAtlasiP37271. baseline and differential.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of an Arabidopsis cDNA for phytoene synthase."
    Scolnik P.A., Bartley G.E.
    Plant Physiol. 104:1471-1472(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence of the phytoene synthase gene of Arabidopsis."
    Castrignano F., Giuliano G.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiPSY_ARATH
AccessioniPrimary (citable) accession number: P37271
Secondary accession number(s): O22375, Q8LE86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 10, 2003
Last modified: June 24, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.