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P37271

- PSY_ARATH

UniProt

P37271 - PSY_ARATH

Protein

Phytoene synthase, chloroplastic

Gene

PSY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (10 Jan 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reaction from prephytoene diphosphate to phytoene.

    Catalytic activityi

    2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carotenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carotenoid biosynthesis, Isoprene biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT5G17230-MONOMER.
    ARA:GQT-752-MONOMER.
    ARA:GQT-753-MONOMER.
    MetaCyc:AT5G17230-MONOMER.
    UniPathwayiUPA00799; UER00773.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phytoene synthase, chloroplastic (EC:2.5.1.32)
    Gene namesi
    Name:PSY1
    Synonyms:PSY
    Ordered Locus Names:At5g17230
    ORF Names:MKP11.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G17230.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7070ChloroplastSequence AnalysisAdd
    BLAST
    Chaini71 – 422352Phytoene synthase, chloroplasticPRO_0000029852Add
    BLAST

    Proteomic databases

    PaxDbiP37271.
    PRIDEiP37271.

    Expressioni

    Gene expression databases

    ArrayExpressiP37271.
    GenevestigatoriP37271.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT5G17230.3-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP37271.
    SMRiP37271. Positions 132-389.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1562.
    HOGENOMiHOG000220848.
    InParanoidiP37271.
    KOiK02291.
    PhylomeDBiP37271.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P37271-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MSSSVAVLWV ATSSLNPDPM NNCGLVRVLE SSRLFSPCQN QRLNKGKKKQ    50
    IPTWSSSFVR NRSRRIGVVS SSLVASPSGE IALSSEEKVY NVVLKQAALV 100
    NKQLRSSSYD LDVKKPQDVV LPGSLSLLGE AYDRCGEVCA EYAKTFYLGT 150
    LLMTPERRKA IWAIYVWCRR TDELVDGPNA SHITPMALDR WEARLEDLFR 200
    GRPFDMLDAA LADTVARYPV DIQPFRDMIE GMRMDLKKSR YQNFDDLYLY 250
    CYYVAGTVGL MSVPVMGIDP KSKATTESVY NAALALGIAN QLTNILRDVG 300
    EDARRGRVYL PQDELAQAGL SDEDIFAGKV TDKWRNFMKM QLKRARMFFD 350
    EAEKGVTELS AASRWPVWAS LLLYRRILDE IEANDYNNFT KRAYVGKVKK 400
    IAALPLAYAK SVLKTSSSRL SI 422
    Length:422
    Mass (Da):47,487
    Last modified:January 10, 2003 - v2
    Checksum:iC44F0A512F2DD31E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti60 – 601R → M in AAM62787. 1 PublicationCurated
    Sequence conflicti128 – 1281L → LV in AAA32836. (PubMed:8016277)Curated
    Sequence conflicti143 – 1431A → P in AAA32836. (PubMed:8016277)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25812 mRNA. Translation: AAA32836.1.
    AF009954 Genomic DNA. Translation: AAB65697.1.
    AB005238 Genomic DNA. Translation: BAB10510.1.
    CP002688 Genomic DNA. Translation: AED92399.1.
    CP002688 Genomic DNA. Translation: AED92400.1.
    BT000450 mRNA. Translation: AAN17427.1.
    BT002084 mRNA. Translation: AAN72095.1.
    AY085565 mRNA. Translation: AAM62787.1.
    RefSeqiNP_001031895.1. NM_001036818.2. [P37271-1]
    NP_197225.1. NM_121729.2. [P37271-1]
    UniGeneiAt.23726.

    Genome annotation databases

    EnsemblPlantsiAT5G17230.1; AT5G17230.1; AT5G17230. [P37271-1]
    AT5G17230.2; AT5G17230.2; AT5G17230. [P37271-1]
    GeneIDi831587.
    KEGGiath:AT5G17230.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25812 mRNA. Translation: AAA32836.1 .
    AF009954 Genomic DNA. Translation: AAB65697.1 .
    AB005238 Genomic DNA. Translation: BAB10510.1 .
    CP002688 Genomic DNA. Translation: AED92399.1 .
    CP002688 Genomic DNA. Translation: AED92400.1 .
    BT000450 mRNA. Translation: AAN17427.1 .
    BT002084 mRNA. Translation: AAN72095.1 .
    AY085565 mRNA. Translation: AAM62787.1 .
    RefSeqi NP_001031895.1. NM_001036818.2. [P37271-1 ]
    NP_197225.1. NM_121729.2. [P37271-1 ]
    UniGenei At.23726.

    3D structure databases

    ProteinModelPortali P37271.
    SMRi P37271. Positions 132-389.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G17230.3-P.

    Proteomic databases

    PaxDbi P37271.
    PRIDEi P37271.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G17230.1 ; AT5G17230.1 ; AT5G17230 . [P37271-1 ]
    AT5G17230.2 ; AT5G17230.2 ; AT5G17230 . [P37271-1 ]
    GeneIDi 831587.
    KEGGi ath:AT5G17230.

    Organism-specific databases

    TAIRi AT5G17230.

    Phylogenomic databases

    eggNOGi COG1562.
    HOGENOMi HOG000220848.
    InParanoidi P37271.
    KOi K02291.
    PhylomeDBi P37271.

    Enzyme and pathway databases

    UniPathwayi UPA00799 ; UER00773 .
    BioCyci ARA:AT5G17230-MONOMER.
    ARA:GQT-752-MONOMER.
    ARA:GQT-753-MONOMER.
    MetaCyc:AT5G17230-MONOMER.

    Gene expression databases

    ArrayExpressi P37271.
    Genevestigatori P37271.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of an Arabidopsis cDNA for phytoene synthase."
      Scolnik P.A., Bartley G.E.
      Plant Physiol. 104:1471-1472(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence of the phytoene synthase gene of Arabidopsis."
      Castrignano F., Giuliano G.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiPSY_ARATH
    AccessioniPrimary (citable) accession number: P37271
    Secondary accession number(s): O22375, Q8LE86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: January 10, 2003
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3