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Protein

Phytoene synthase, chloroplastic

Gene

PSY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reaction from prephytoene diphosphate to phytoene.1 Publication

Catalytic activityi

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.1 Publication

Pathwayi: phytoene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate.Curated
Proteins known to be involved in this subpathway in this organism are:
  1. Phytoene synthase, chloroplastic (PSY1)
This subpathway is part of the pathway phytoene biosynthesis, which is itself part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate, the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • carotenoid biosynthetic process Source: UniProtKB
  • ergosterol biosynthetic process Source: InterPro

Keywordsi

Molecular functionTransferase
Biological processCarotenoid biosynthesis, Isoprene biosynthesis

Enzyme and pathway databases

BioCyciARA:AT5G17230-MONOMER.
MetaCyc:AT5G17230-MONOMER.
BRENDAi2.5.1.32. 399.
UniPathwayiUPA00799; UER00773.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene synthase, chloroplastic (EC:2.5.1.321 Publication)
Gene namesi
Name:PSY1
Synonyms:PSY
Ordered Locus Names:At5g17230
ORF Names:MKP11.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G17230.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70ChloroplastSequence analysisAdd BLAST70
ChainiPRO_000002985271 – 422Phytoene synthase, chloroplasticAdd BLAST352

Proteomic databases

PaxDbiP37271.

Expressioni

Gene expression databases

ExpressionAtlasiP37271. baseline and differential.
GenevisibleiP37271. AT.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with OR (PubMed:26224804, PubMed:25675505). Interacts with ORLIKE (PubMed:25675505).By similarity2 Publications

Protein-protein interaction databases

BioGridi16863. 3 interactors.
STRINGi3702.AT5G17230.3.

Structurei

3D structure databases

ProteinModelPortaliP37271.
SMRiP37271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1459. Eukaryota.
COG1562. LUCA.
HOGENOMiHOG000220848.
InParanoidiP37271.
KOiK02291.
PhylomeDBiP37271.

Family and domain databases

CDDicd00683. Trans_IPPS_HH. 1 hit.
Gene3Di1.10.600.10. 1 hit.
InterProiView protein in InterPro
IPR008949. Isoprenoid_synthase_dom_sf.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR033904. Trans_IPPS_HH.
PfamiView protein in Pfam
PF00494. SQS_PSY. 1 hit.
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiView protein in PROSITE
PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P37271-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSVAVLWV ATSSLNPDPM NNCGLVRVLE SSRLFSPCQN QRLNKGKKKQ
60 70 80 90 100
IPTWSSSFVR NRSRRIGVVS SSLVASPSGE IALSSEEKVY NVVLKQAALV
110 120 130 140 150
NKQLRSSSYD LDVKKPQDVV LPGSLSLLGE AYDRCGEVCA EYAKTFYLGT
160 170 180 190 200
LLMTPERRKA IWAIYVWCRR TDELVDGPNA SHITPMALDR WEARLEDLFR
210 220 230 240 250
GRPFDMLDAA LADTVARYPV DIQPFRDMIE GMRMDLKKSR YQNFDDLYLY
260 270 280 290 300
CYYVAGTVGL MSVPVMGIDP KSKATTESVY NAALALGIAN QLTNILRDVG
310 320 330 340 350
EDARRGRVYL PQDELAQAGL SDEDIFAGKV TDKWRNFMKM QLKRARMFFD
360 370 380 390 400
EAEKGVTELS AASRWPVWAS LLLYRRILDE IEANDYNNFT KRAYVGKVKK
410 420
IAALPLAYAK SVLKTSSSRL SI
Length:422
Mass (Da):47,487
Last modified:January 10, 2003 - v2
Checksum:iC44F0A512F2DD31E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60R → M in AAM62787 (Ref. 6) Curated1
Sequence conflicti128L → LV in AAA32836 (PubMed:8016277).Curated1
Sequence conflicti143A → P in AAA32836 (PubMed:8016277).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25812 mRNA. Translation: AAA32836.1.
AF009954 Genomic DNA. Translation: AAB65697.1.
AB005238 Genomic DNA. Translation: BAB10510.1.
CP002688 Genomic DNA. Translation: AED92399.1.
CP002688 Genomic DNA. Translation: AED92400.1.
CP002688 Genomic DNA. Translation: ANM69856.1.
BT000450 mRNA. Translation: AAN17427.1.
BT002084 mRNA. Translation: AAN72095.1.
AY085565 mRNA. Translation: AAM62787.1.
RefSeqiNP_001031895.1. NM_001036818.2. [P37271-1]
NP_001331504.1. NM_001343484.1. [P37271-1]
NP_197225.1. NM_121729.3. [P37271-1]
UniGeneiAt.23726.

Genome annotation databases

EnsemblPlantsiAT5G17230.1; AT5G17230.1; AT5G17230. [P37271-1]
AT5G17230.2; AT5G17230.2; AT5G17230. [P37271-1]
AT5G17230.4; AT5G17230.4; AT5G17230. [P37271-1]
GeneIDi831587.
GrameneiAT5G17230.1; AT5G17230.1; AT5G17230.
AT5G17230.2; AT5G17230.2; AT5G17230.
AT5G17230.4; AT5G17230.4; AT5G17230.
KEGGiath:AT5G17230.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSY_ARATH
AccessioniPrimary (citable) accession number: P37271
Secondary accession number(s): O22375, Q8LE86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 10, 2003
Last modified: November 22, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families