Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P37271 (PSY_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phytoene synthase, chloroplastic

EC=2.5.1.32
Gene names
Name:PSY1
Synonyms:PSY
Ordered Locus Names:At5g17230
ORF Names:MKP11.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the reaction from prephytoene diphosphate to phytoene.

Catalytic activity

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathway

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Subunit structure

Monomer By similarity.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
   Biological processCarotenoid biosynthesis
Isoprene biosynthesis
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   Molecular functionTransferase
   Technical termComplete proteome
Multifunctional enzyme
Reference proteome
Gene Ontology (GO)
   Biological_processcarotenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiongeranylgeranyl-diphosphate geranylgeranyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P37271-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7070Chloroplast Potential
Chain71 – 422352Phytoene synthase, chloroplastic
PRO_0000029852

Experimental info

Sequence conflict601R → M in AAM62787. Ref.6
Sequence conflict1281L → LV in AAA32836. Ref.1
Sequence conflict1431A → P in AAA32836. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 10, 2003. Version 2.
Checksum: C44F0A512F2DD31E

FASTA42247,487
        10         20         30         40         50         60 
MSSSVAVLWV ATSSLNPDPM NNCGLVRVLE SSRLFSPCQN QRLNKGKKKQ IPTWSSSFVR 

        70         80         90        100        110        120 
NRSRRIGVVS SSLVASPSGE IALSSEEKVY NVVLKQAALV NKQLRSSSYD LDVKKPQDVV 

       130        140        150        160        170        180 
LPGSLSLLGE AYDRCGEVCA EYAKTFYLGT LLMTPERRKA IWAIYVWCRR TDELVDGPNA 

       190        200        210        220        230        240 
SHITPMALDR WEARLEDLFR GRPFDMLDAA LADTVARYPV DIQPFRDMIE GMRMDLKKSR 

       250        260        270        280        290        300 
YQNFDDLYLY CYYVAGTVGL MSVPVMGIDP KSKATTESVY NAALALGIAN QLTNILRDVG 

       310        320        330        340        350        360 
EDARRGRVYL PQDELAQAGL SDEDIFAGKV TDKWRNFMKM QLKRARMFFD EAEKGVTELS 

       370        380        390        400        410        420 
AASRWPVWAS LLLYRRILDE IEANDYNNFT KRAYVGKVKK IAALPLAYAK SVLKTSSSRL 


SI 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of an Arabidopsis cDNA for phytoene synthase."
Scolnik P.A., Bartley G.E.
Plant Physiol. 104:1471-1472(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence of the phytoene synthase gene of Arabidopsis."
Castrignano F., Giuliano G.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Columbia.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L25812 mRNA. Translation: AAA32836.1.
AF009954 Genomic DNA. Translation: AAB65697.1.
AB005238 Genomic DNA. Translation: BAB10510.1.
CP002688 Genomic DNA. Translation: AED92399.1.
CP002688 Genomic DNA. Translation: AED92400.1.
BT000450 mRNA. Translation: AAN17427.1.
BT002084 mRNA. Translation: AAN72095.1.
AY085565 mRNA. Translation: AAM62787.1.
RefSeqNP_001031895.1. NM_001036818.2.
NP_197225.1. NM_121729.2.
UniGeneAt.23726.

3D structure databases

ProteinModelPortalP37271.
SMRP37271. Positions 132-389.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G17230.3-P.

Proteomic databases

PaxDbP37271.
PRIDEP37271.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G17230.1; AT5G17230.1; AT5G17230. [P37271-1]
AT5G17230.2; AT5G17230.2; AT5G17230. [P37271-1]
GeneID831587.
KEGGath:AT5G17230.

Organism-specific databases

TAIRAT5G17230.

Phylogenomic databases

eggNOGCOG1562.
HOGENOMHOG000220848.
InParanoidP37271.
KOK02291.
PhylomeDBP37271.
ProtClustDBPLN02632.

Enzyme and pathway databases

BioCycARA:AT5G17230-MONOMER.
ARA:GQT-752-MONOMER.
ARA:GQT-753-MONOMER.
MetaCyc:AT5G17230-MONOMER.
UniPathwayUPA00799; UER00773.

Gene expression databases

ArrayExpressP37271.
GenevestigatorP37271.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSY_ARATH
AccessionPrimary (citable) accession number: P37271
Secondary accession number(s): O22375, Q8LE86
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 10, 2003
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names