P37270 (CRTB_THET2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytoene synthase
EC=2.5.1.32

Gene names

Name:	crtB
Ordered Locus Names:	TT_P0057

Encoded on

Plasmid pTT27 Ref.2

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene.
Catalytic activity	2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme Plasmid
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Phytoene synthase		PRO_0000067439

Sequences

	Sequence		Length	Mass (Da)
	P37270 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 3E58C1141902D3C5	FASTA	289	31,964
	10 20 30 40 50 60 MKMPASMEPD WKALLRVLRA HSATFYLGSL LFPKEARKGA WAVYAACRLG DEAVDGEGGG 70 80 90 100 110 120 PEALEAWWAG VERAYRGRPL AEWEKGLAWA LERWDIPFEA FLHMREGFLT DLGPVRLGTE 130 140 150 160 170 180 AELLRYCYQV AGTVGRMMAP IAGGGKEAEA RAVKLGQAMQ LTNILRDVGE DLERDRVYLP 190 200 210 220 230 240 LDLLRAHGVE VEDLRAGRVT PGYRALMAHL EGKARALYRE GLAGLGHLKV GRAAIALAAL 250 260 270 280 QYRGILDKLR LSGYDNLGRR AHLKAWERAL LLPKAFLAAR FPPRPEGSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence analysis of the crtB gene of Thermus thermophilus HB27, an extreme thermophile producing carotenoid pigments." Hoshino T., Fujii R., Nakahara T. Appl. Environ. Microbiol. 59:3150-3153(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB27 / ATCC BAA-163 / DSM 7039.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB001637 Genomic DNA. No translation available. AE017222 Genomic DNA. Translation: AAS82387.1.
RefSeq	YP_006040.1. NC_005838.1.
3D structure databases
ProteinModelPortal	P37270.
ModBase	Search...
Protein-protein interaction databases
STRING	262724.TT_P0057.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS82387; AAS82387; TT_P0057.
GeneID	2776542.
KEGG	tth:TT_P0057.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1562.
HOGENOM	HOG000220848.
KO	K02291.
OMA	KGAWAVY.
ProtClustDB	CLSK444753.
Enzyme and pathway databases
UniPathway	UPA00799; UER00773.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTB_THET2

Accession

Primary (citable) accession number: P37270

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program