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Protein

15-cis-phytoene synthase

Gene

crtB

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield 15-cis-phytoene.1 Publication

Caution

The enzyme produces 15-cis-phytoene. The conversion to all-trans-phytoene is due to photoisomerisation.By similarity

Catalytic activityi

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • ATPBy similarityNote: ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction.By similarity
  • Mn2+By similarity, Mg2+By similarity

Pathwayi: phytoene biosynthesis

This protein is involved in the pathway phytoene biosynthesis, which is part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • carotenoid biosynthetic process Source: UniProtKB
  • ergosterol biosynthetic process Source: InterPro

Keywordsi

Molecular functionTransferase
Biological processCarotenoid biosynthesis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:SYNPCC7942_1984-MONOMER
SYNEL:SYNPCC7942_1984-MONOMER
UniPathwayiUPA00799

Names & Taxonomyi

Protein namesi
Recommended name:
15-cis-phytoene synthaseCurated (EC:2.5.1.321 Publication)
Short name:
PSase
Gene namesi
Name:crtB
Synonyms:pys
Ordered Locus Names:Synpcc7942_1984
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002717 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000674371 – 30815-cis-phytoene synthaseAdd BLAST308

Proteomic databases

PRIDEiP37269

Interactioni

Protein-protein interaction databases

STRINGi1140.Synpcc7942_1984

Structurei

3D structure databases

ProteinModelPortaliP37269
SMRiP37269
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105FE1 Bacteria
COG1562 LUCA
HOGENOMiHOG000220848
KOiK02291
OrthoDBiPOG091H0EAX

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P37269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQMIPARPS RALASLSEAY EECRQITARY AKTFYLGTLL MPEAKRQAIW
60 70 80 90 100
AIYVWCRRTD ELVDGPQAAQ TNFATLDAWE RRLERLFAGE PEDDCDVALV
110 120 130 140 150
DTLARYPLDI QPFRDMIEGQ RMDLLQNRYS TFEDLYTYCY RVAGTVGLMS
160 170 180 190 200
QPVMGIESTN SRAPWDPTTP PDPTQEALAL GIANQLTNIL RDVGEDARRG
210 220 230 240 250
RIYLPQEELA QFNYSEQDLF NGVIDDRWRA FMQFQLDRAR DYFEQAERGI
260 270 280 290 300
RQLSHDARWP VWASLMLYRE ILDVIEQNNY DVFRKRAYVP TWRKLCSLPV

AMLRATVL
Length:308
Mass (Da):35,891
Last modified:April 18, 2006 - v2
Checksum:i9399E7308C4FFC18
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti249G → C (PubMed:1537409).Curated1
Sequence conflicti251 – 252RQ → E (PubMed:1537409).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63873 Genomic DNA Translation: CAA45350.1
CP000100 Genomic DNA Translation: ABB58014.1
PIRiS20383
RefSeqiWP_011244422.1, NC_007604.1

Genome annotation databases

EnsemblBacteriaiABB58014; ABB58014; Synpcc7942_1984
KEGGisyf:Synpcc7942_1984

Similar proteinsi

Entry informationi

Entry nameiCRTB_SYNE7
AccessioniPrimary (citable) accession number: P37269
Secondary accession number(s): Q31LQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 18, 2006
Last modified: April 25, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health