P37267 (CBP4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Assembly factor CBP4

Alternative name(s):
Cytochrome b mRNA-processing protein 4

Gene names

Name:	CBP4
Ordered Locus Names:	YGR174C
ORF Names:	G7122

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the assembly of ubiquinol-cytochrome c reductase. It has a direct effect on the correct occurrence of the Rieske protein, core 4, core 5 and apocytochrome b. Ref.1 Ref.9
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein Potential Ref.1 Ref.6 Ref.8.
Miscellaneous	Present with 704 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CBP4 family.
Sequence caution	The sequence AAA61566.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAT93181.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAA97200.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence DAA08269.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial respiratory chain complex III assembly Inferred from mutant phenotype Ref.9Ref.1. Source: SGD
Cellular_component	integral to mitochondrial membrane Inferred from direct assay Ref.1. Source: SGD mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Assembly factor CBP4		PRO_0000020845
Regions
	Transmembrane	5 – 27	23	Helical; Potential
Experimental info
	Sequence conflict	42	1	S → F in AAA61566. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37267 [UniParc]. Last modified October 2, 2007. Version 3. Checksum: 27CD71BD4C7CE8BB	FASTA	147	17,532
	10 20 30 40 50 60 MERPLWVRWL KVYAIGGAII GSGFLLFKYT TPTDQQLISQ LSPELRLQYE REKKLRQSEQ 70 80 90 100 110 120 QALMKIVKET SQSDDPIWKT GPLQSPWERN GDNVQSRDHF AKVRAEEVQK EELARIRNEL 130 140 SQLRSETEEK TKEIVQDKQV KSWWRFW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of CBP4, a new gene essential for the expression of ubiquinol-cytochrome c reductase in Saccharomyces cerevisiae." Crivellone M.D. J. Biol. Chem. 269:21284-21292(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Verification of 3' and 5' ends of S.cerevisiae transcripts." Kennedy M.C., Dietrich F.S. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-43 AND 91-147. Strain: ATCC 204511 / S288c / AB972.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[9]	"Organization of assembly factors Cbp3p and Cbp4p and their effect on bc(1) complex assembly in Saccharomyces cerevisiae." Kronekova Z., Rodel G. Curr. Genet. 47:203-212(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CBP3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10700 Genomic DNA. Translation: AAA61566.1. Different initiation. Z72959 Genomic DNA. Translation: CAA97200.1. Different initiation. AY693162 Genomic DNA. Translation: AAT93181.1. Different initiation. AY389299 mRNA. Translation: AAQ97231.1. AY389298 mRNA. Translation: AAQ97230.1. BK006941 Genomic DNA. Translation: DAA08269.1. Different initiation.
PIR	S64488.
RefSeq	NP_011690.3. NM_001181303.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4216N.
MINT	MINT-574347.
Proteomic databases
PaxDb	P37267.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	853085.
KEGG	sce:YGR174C. sce:YGR180C.
Organism-specific databases
CYGD	YGR174c.
SGD	S000003406. CBP4.
Phylogenomic databases
eggNOG	NOG83063.
HOGENOM	HOG000065984.
KO	K10808.
OrthoDB	EOG4ZPJ4W.
Gene expression databases
Genevestigator	P37267.
GermOnline	YGR174C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012420. Cbp4. [Graphical view]
Pfam	PF07960. CBP4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973061.

Entry information

Entry name

CBP4_YEAST

Accession

Primary (citable) accession number: P37267
Secondary accession number(s): D6VUV8, Q6TQT9, Q6TQU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 2, 2007
Last modified:	April 3, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program