Reviewed,
UniProtKB/Swiss-Prot P37254 (PABS_YEAST)
Last modified
November 25, 2008.
Version 93.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Aminodeoxychorismate synthase Short name=ADC synthase EC=2.6.1.85 Alternative name(s): Para-aminobenzoate synthase P-aminobenzoic acid synthase Short name=PABA synthase | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 787 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine. Required for the synthesis of 4-aminobenzoate (PABA), an important component in tetrahydrofolate biosynthesis. |
| Catalytic activity | Chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate. |
| Pathway | Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2. |
| Subcellular location | |
| Domain | The PABA component provides the glutamine amidotransferase activity. The PABB component catalyzes the formation of ADC by binding chorismate and ammonia. |
| Miscellaneous | Present with 1550 molecules/cell in log phase SD medium. |
| Sequence similarities | In the C-terminal section; belongs to the anthranilate synthase component I family. Contains 1 glutamine amidotransferase type-1 domain. |
| Sequence caution | The sequence AAA34840.1 differs from that shown. Reason: Frameshift at positions 581, 586, 591, 716, 729 and 734. The sequence AAB49319.1 differs from that shown. Reason: Frameshift at positions 581, 586 and 591. |
Ontologies
Keywords | |
|---|---|
| Biological process | Folate biosynthesis |
| Cellular component | Cytoplasm |
| Domain | Glutamine amidotransferase |
| Molecular function | Transferase |
| Technical term | Complete proteome Multifunctional enzyme |
Gene Ontology (GO) | |
| Biological process | folic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic processInferred from electronic annotation. Source: InterPro para-aminobenzoic acid metabolic process Ref.1Inferred from mutant phenotype. Source: SGD |
| Cellular component | cytoplasm Ref.5 Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 4-amino-4-deoxychorismate synthase activity Ref.1 Inferred from mutant phenotype. Source: SGD anthranilate synthase activityInferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct transferase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ESS1 | P22696 | 1 | EBI-12831,EBI-6679 | |
| PRP40 | P33203 | 1 | EBI-12831,EBI-701 | |
| SSM4 | P40318 | 1 | EBI-12831,EBI-18208 | |
| URN1 | Q06525 | 1 | EBI-12831,EBI-35138 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 787 | 787 | Aminodeoxychorismate synthase | PRO_0000154142 | |||||
Regions | |||||||||
| Domain | 16 – 233 | 218 | Glutamine amidotransferase type-1 | ||||||
| Region | 304 – 787 | 484 | PABB component | ||||||
| Compositional bias | 10 – 14 | 5 | Poly-Gln | ||||||
Sites | |||||||||
| Active site | 112 | 1 | By similarity | ||||||
| Active site | 207 | 1 | By similarity | ||||||
| Active site | 209 | 1 | By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 85 | 1 | G → D in AAT93048. Ref.4 | ||||||
| Sequence conflict | 171 | 1 | A → P in AAA34840. Ref.1 | ||||||
| Sequence conflict | 171 | 1 | A → P in AAB49319. Ref.2 | ||||||
| Sequence conflict | 533 | 1 | A → T in AAA34840. Ref.1 | ||||||
| Sequence conflict | 586 | 1 | D → Y in AAB49319. Ref.2 | ||||||
| Sequence conflict | 590 – 591 | 2 | CE → FV in AAA34840. Ref.1 | ||||||
| Sequence conflict | 721 | 1 | G → GL in AAB49319. Ref.2 | ||||||
| Sequence conflict | 722 | 1 | V → G in AAA34840. Ref.1 | ||||||
| Sequence conflict | 735 – 737 | 3 | NGD → FGF in AAA34840. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Para-aminobenzoate synthase gene of Saccharomyces cerevisiae encodes a bifunctional enzyme." Edman J.C., Goldstein A.L., Erbe J.G. Yeast 9:669-675(1993) [PubMed: 8346682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. |
| [2] | "Los1p, involved in yeast pre-tRNA splicing, positively regulates members of the SOL gene family." Shen W.-C., Stanford D.R., Hopper A.K. Genetics 143:699-712(1996) [PubMed: 8725220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| L15299 Genomic DNA. Translation: AAA34840.1. Frameshift. U43608 Genomic DNA. Translation: AAB49319.1. Frameshift. Z71648 Genomic DNA. Translation: CAA96313.1. AY693029 Genomic DNA. Translation: AAT93048.1. | |
| PIR | S63364. |
| RefSeq | NP_014431.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1K0E based on UniProtKB P05041. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:6278N. |
| IntAct | P37254. |
Proteomic databases | |
| PeptideAtlas | P37254. |
Genome annotation databases | |
| Ensembl | YNR033W. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 855768. |
| GenomeReviews | Gene locus YNR033W in contig Y13139_GR. |
| KEGG | sce:YNR033W. |
| NMPDR | fig|4932.3.peg.5511. |
Organism-specific databases | |
| CYGD | YNR033w. |
| SGD | S000005316. ABZ1. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P37254. |
Gene expression databases | |
| ArrayExpress | P37254. |
| GermOnline | YNR033W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR006805. Anth_synth_I_N. IPR006220. Anth_synthII. IPR015890. Chorismate-bd_C. IPR011702. GATASE. IPR012998. GATase_1_AS. IPR000991. GATase_class1_C. IPR010117. PabB_fungal. IPR005801. TRPE_1_chor_bd. IPR006221. TrpG_papA. [Graphical view] |
| Gene3D | G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit. |
| PANTHER | PTHR11236. TRPE_1_chor_bd. 1 hit. |
| Pfam | PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. PF00117. GATase. 1 hit. [Graphical view] |
| PRINTS | PR00097. ANTSNTHASEII. PR00096. GATASE. |
| ProDom | PD000779. Anth_synth_chor. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR01823. PabB-fungal. 1 hit. TIGR00566. trpG_papA. 1 hit. |
| PROSITE | PS51273. GATASE_TYPE_1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00259. Sulfanilamide. |
| LinkHub | P37254. |
| NextBio | 980214. |
Entry information
| Entry name | PABS_YEAST | ||||||||
| Accession | Primary (citable) accession number: P37254 Secondary accession number(s): Q02982, Q6B1Q1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |

Clusters with


