Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aminodeoxychorismate synthase

Gene

ABZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine. Required for the synthesis of 4-aminobenzoate (PABA), an important component in tetrahydrofolate biosynthesis.1 Publication

Catalytic activityi

Chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei112 – 1121PROSITE-ProRule annotation
Active sitei207 – 2071PROSITE-ProRule annotation
Active sitei209 – 2091PROSITE-ProRule annotation

GO - Molecular functioni

  1. 4-amino-4-deoxychorismate synthase activity Source: SGD
  2. oxo-acid-lyase activity Source: InterPro

GO - Biological processi

  1. folic acid biosynthetic process Source: UniProtKB-KW
  2. glutamine metabolic process Source: UniProtKB-KW
  3. para-aminobenzoic acid biosynthetic process Source: SGD
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Folate biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YNR033W-MONOMER.
UniPathwayiUPA00077; UER00149.

Protein family/group databases

MEROPSiC26.958.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminodeoxychorismate synthase (EC:2.6.1.85)
Short name:
ADC synthase
Alternative name(s):
P-aminobenzoic acid synthase
Short name:
PABA synthase
Para-aminobenzoate synthase
Gene namesi
Name:ABZ1
Ordered Locus Names:YNR033W
ORF Names:N3286
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR033w.
SGDiS000005316. ABZ1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 787787Aminodeoxychorismate synthasePRO_0000154142Add
BLAST

Proteomic databases

MaxQBiP37254.
PaxDbiP37254.
PeptideAtlasiP37254.

Expressioni

Gene expression databases

GenevestigatoriP37254.

Interactioni

Protein-protein interaction databases

BioGridi35858. 48 interactions.
DIPiDIP-6278N.
IntActiP37254. 9 interactions.
MINTiMINT-604611.

Structurei

3D structure databases

ProteinModelPortaliP37254.
SMRiP37254. Positions 17-224, 492-786.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 233218Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni304 – 787484PABB componentAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 145Poly-Gln

Domaini

The PABA component provides the glutamine amidotransferase activity.
The PABB component catalyzes the formation of ADC by binding chorismate and ammonia.

Sequence similaritiesi

In the C-terminal section; belongs to the anthranilate synthase component I family.Curated
Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0147.
GeneTreeiENSGT00620000088738.
HOGENOMiHOG000025143.
InParanoidiP37254.
KOiK13950.
OMAiEGEWDEM.
OrthoDBiEOG7PZS5V.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR006805. Anth_synth_I_N.
IPR015890. Chorismate_C.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010117. PabB_fungal.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR01823. PabB-fungal. 1 hit.
TIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37254-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSDTIDTKQ QQQQLHVLFI DSYDSFTYNV VRLIEQQTDI SPGVNAVHVT
60 70 80 90 100
TVHSDTFQSM DQLLPLLPLF DAIVVGPGPG NPNNGAQDMG IISELFENAN
110 120 130 140 150
GKLDEVPILG ICLGFQAMCL AQGADVSELN TIKHGQVYEM HLNDAARACG
160 170 180 190 200
LFSGYPDTFK STRYHSLHVN AEGIDTLLPL CTTEDENGIL LMSAQTKNKP
210 220 230 240 250
WFGVQYHPES CCSELGGLLV SNFLKLSFIN NVKTGRWEKK KLNGEFSDIL
260 270 280 290 300
SRLDRTIDRD PIYKVKEKYP KGEDTTYVKQ FEVSEDPKLT FEICNIIREE
310 320 330 340 350
KFVMSSSVIS ENTGEWSIIA LPNSASQVFT HYGAMKKTTV HYWQDSEISY
360 370 380 390 400
TLLKKCLDGQ DSDLPGSLEV IHEDKSQFWI TLGKFMENKI IDNHREIPFI
410 420 430 440 450
GGLVGILGYE IGQYIACGRC NDDENSLVPD AKLVFINNSI VINHKQGKLY
460 470 480 490 500
CISLDNTFPV ALEQSLRDSF VRKKNIKQSL SWPKYLPEEI DFIITMPDKL
510 520 530 540 550
DYAKAFKKCQ DYMHKGDSYE MCLTTQTKVV PSAVIEPWRI FQTLVQRNPA
560 570 580 590 600
PFSSFFEFKD IIPRQDETPP VLCFLSTSPE RFLKWDADTC ELRPIKGTVK
610 620 630 640 650
KGPQMNLAKA TRILKTPKEF GENLMILDLI RNDLYELVPD VRVEEFMSVQ
660 670 680 690 700
EYATVYQLVS VVKAHGLTSA SKKTRYSGID VLKHSLPPGS MTGAPKKITV
710 720 730 740 750
QLLQDKIESK LNKHVNGGAR GVYSGVTGYW SVNSNGDWSV NIRCMYSYNG
760 770 780
GTSWQLGAGG AITVLSTLDG ELEEMYNKLE SNLQIFM
Length:787
Mass (Da):88,544
Last modified:February 21, 2006 - v4
Checksum:i13117891486B5B0E
GO

Sequence cautioni

The sequence AAA34840.1 differs from that shown. Reason: Frameshift at positions 581, 586, 591, 716, 729 and 734. Curated
The sequence AAB49319.1 differs from that shown. Reason: Frameshift at positions 581, 586 and 591. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851G → D in AAT93048. (PubMed:17322287)Curated
Sequence conflicti171 – 1711A → P in AAA34840. (PubMed:8346682)Curated
Sequence conflicti171 – 1711A → P in AAB49319. (PubMed:8725220)Curated
Sequence conflicti533 – 5331A → T in AAA34840. (PubMed:8346682)Curated
Sequence conflicti586 – 5861D → Y in AAB49319. (PubMed:8725220)Curated
Sequence conflicti590 – 5912CE → FV in AAA34840. (PubMed:8346682)Curated
Sequence conflicti721 – 7211G → GL in AAB49319. (PubMed:8725220)Curated
Sequence conflicti722 – 7221V → G in AAA34840. (PubMed:8346682)Curated
Sequence conflicti735 – 7373NGD → FGF in AAA34840. (PubMed:8346682)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15299 Genomic DNA. Translation: AAA34840.1. Frameshift.
U43608 Genomic DNA. Translation: AAB49319.1. Frameshift.
Z71648 Genomic DNA. Translation: CAA96313.1.
AY693029 Genomic DNA. Translation: AAT93048.1.
BK006947 Genomic DNA. Translation: DAA10574.1.
PIRiS63364.
RefSeqiNP_014431.1. NM_001183210.1.

Genome annotation databases

EnsemblFungiiYNR033W; YNR033W; YNR033W.
GeneIDi855768.
KEGGisce:YNR033W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15299 Genomic DNA. Translation: AAA34840.1. Frameshift.
U43608 Genomic DNA. Translation: AAB49319.1. Frameshift.
Z71648 Genomic DNA. Translation: CAA96313.1.
AY693029 Genomic DNA. Translation: AAT93048.1.
BK006947 Genomic DNA. Translation: DAA10574.1.
PIRiS63364.
RefSeqiNP_014431.1. NM_001183210.1.

3D structure databases

ProteinModelPortaliP37254.
SMRiP37254. Positions 17-224, 492-786.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35858. 48 interactions.
DIPiDIP-6278N.
IntActiP37254. 9 interactions.
MINTiMINT-604611.

Protein family/group databases

MEROPSiC26.958.

Proteomic databases

MaxQBiP37254.
PaxDbiP37254.
PeptideAtlasiP37254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR033W; YNR033W; YNR033W.
GeneIDi855768.
KEGGisce:YNR033W.

Organism-specific databases

CYGDiYNR033w.
SGDiS000005316. ABZ1.

Phylogenomic databases

eggNOGiCOG0147.
GeneTreeiENSGT00620000088738.
HOGENOMiHOG000025143.
InParanoidiP37254.
KOiK13950.
OMAiEGEWDEM.
OrthoDBiEOG7PZS5V.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00149.
BioCyciYEAST:YNR033W-MONOMER.

Miscellaneous databases

NextBioi980214.

Gene expression databases

GenevestigatoriP37254.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR006805. Anth_synth_I_N.
IPR015890. Chorismate_C.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010117. PabB_fungal.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR01823. PabB-fungal. 1 hit.
TIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Para-aminobenzoate synthase gene of Saccharomyces cerevisiae encodes a bifunctional enzyme."
    Edman J.C., Goldstein A.L., Erbe J.G.
    Yeast 9:669-675(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "Los1p, involved in yeast pre-tRNA splicing, positively regulates members of the SOL gene family."
    Shen W.-C., Stanford D.R., Hopper A.K.
    Genetics 143:699-712(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPABS_YEAST
AccessioniPrimary (citable) accession number: P37254
Secondary accession number(s): D6W1K8, Q02982, Q6B1Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 21, 2006
Last modified: January 7, 2015
This is version 148 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.