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P37254

- PABS_YEAST

UniProt

P37254 - PABS_YEAST

Protein

Aminodeoxychorismate synthase

Gene

ABZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 4 (21 Feb 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine. Required for the synthesis of 4-aminobenzoate (PABA), an important component in tetrahydrofolate biosynthesis.1 Publication

    Catalytic activityi

    Chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei112 – 1121PROSITE-ProRule annotation
    Active sitei207 – 2071PROSITE-ProRule annotation
    Active sitei209 – 2091PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4-amino-4-deoxychorismate synthase activity Source: SGD
    2. oxo-acid-lyase activity Source: InterPro

    GO - Biological processi

    1. folic acid biosynthetic process Source: UniProtKB-KW
    2. glutamine metabolic process Source: UniProtKB-KW
    3. para-aminobenzoic acid biosynthetic process Source: SGD
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Folate biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YNR033W-MONOMER.
    UniPathwayiUPA00077; UER00149.

    Protein family/group databases

    MEROPSiC26.958.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminodeoxychorismate synthase (EC:2.6.1.85)
    Short name:
    ADC synthase
    Alternative name(s):
    P-aminobenzoic acid synthase
    Short name:
    PABA synthase
    Para-aminobenzoate synthase
    Gene namesi
    Name:ABZ1
    Ordered Locus Names:YNR033W
    ORF Names:N3286
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR033w.
    SGDiS000005316. ABZ1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 787787Aminodeoxychorismate synthasePRO_0000154142Add
    BLAST

    Proteomic databases

    MaxQBiP37254.
    PaxDbiP37254.
    PeptideAtlasiP37254.

    Expressioni

    Gene expression databases

    GenevestigatoriP37254.

    Interactioni

    Protein-protein interaction databases

    BioGridi35858. 47 interactions.
    DIPiDIP-6278N.
    IntActiP37254. 9 interactions.
    MINTiMINT-604611.

    Structurei

    3D structure databases

    ProteinModelPortaliP37254.
    SMRiP37254. Positions 17-224, 492-786.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 233218Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni304 – 787484PABB componentAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi10 – 145Poly-Gln

    Domaini

    The PABA component provides the glutamine amidotransferase activity.
    The PABB component catalyzes the formation of ADC by binding chorismate and ammonia.

    Sequence similaritiesi

    In the C-terminal section; belongs to the anthranilate synthase component I family.Curated
    Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0147.
    GeneTreeiENSGT00620000088738.
    HOGENOMiHOG000025143.
    KOiK13950.
    OMAiYEANDAT.
    OrthoDBiEOG7PZS5V.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    3.60.120.10. 1 hit.
    InterProiIPR005801. ADC_synthase.
    IPR006805. Anth_synth_I_N.
    IPR015890. Chorismate-bd_C.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010117. PabB_fungal.
    IPR006221. TrpG/PapA_dom.
    [Graphical view]
    PfamiPF04715. Anth_synt_I_N. 1 hit.
    PF00425. Chorismate_bind. 1 hit.
    PF00117. GATase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    SSF56322. SSF56322. 1 hit.
    TIGRFAMsiTIGR01823. PabB-fungal. 1 hit.
    TIGR00566. trpG_papA. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P37254-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSDTIDTKQ QQQQLHVLFI DSYDSFTYNV VRLIEQQTDI SPGVNAVHVT    50
    TVHSDTFQSM DQLLPLLPLF DAIVVGPGPG NPNNGAQDMG IISELFENAN 100
    GKLDEVPILG ICLGFQAMCL AQGADVSELN TIKHGQVYEM HLNDAARACG 150
    LFSGYPDTFK STRYHSLHVN AEGIDTLLPL CTTEDENGIL LMSAQTKNKP 200
    WFGVQYHPES CCSELGGLLV SNFLKLSFIN NVKTGRWEKK KLNGEFSDIL 250
    SRLDRTIDRD PIYKVKEKYP KGEDTTYVKQ FEVSEDPKLT FEICNIIREE 300
    KFVMSSSVIS ENTGEWSIIA LPNSASQVFT HYGAMKKTTV HYWQDSEISY 350
    TLLKKCLDGQ DSDLPGSLEV IHEDKSQFWI TLGKFMENKI IDNHREIPFI 400
    GGLVGILGYE IGQYIACGRC NDDENSLVPD AKLVFINNSI VINHKQGKLY 450
    CISLDNTFPV ALEQSLRDSF VRKKNIKQSL SWPKYLPEEI DFIITMPDKL 500
    DYAKAFKKCQ DYMHKGDSYE MCLTTQTKVV PSAVIEPWRI FQTLVQRNPA 550
    PFSSFFEFKD IIPRQDETPP VLCFLSTSPE RFLKWDADTC ELRPIKGTVK 600
    KGPQMNLAKA TRILKTPKEF GENLMILDLI RNDLYELVPD VRVEEFMSVQ 650
    EYATVYQLVS VVKAHGLTSA SKKTRYSGID VLKHSLPPGS MTGAPKKITV 700
    QLLQDKIESK LNKHVNGGAR GVYSGVTGYW SVNSNGDWSV NIRCMYSYNG 750
    GTSWQLGAGG AITVLSTLDG ELEEMYNKLE SNLQIFM 787
    Length:787
    Mass (Da):88,544
    Last modified:February 21, 2006 - v4
    Checksum:i13117891486B5B0E
    GO

    Sequence cautioni

    The sequence AAA34840.1 differs from that shown. Reason: Frameshift at positions 581, 586, 591, 716, 729 and 734.
    The sequence AAB49319.1 differs from that shown. Reason: Frameshift at positions 581, 586 and 591.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851G → D in AAT93048. (PubMed:17322287)Curated
    Sequence conflicti171 – 1711A → P in AAA34840. (PubMed:8346682)Curated
    Sequence conflicti171 – 1711A → P in AAB49319. (PubMed:8725220)Curated
    Sequence conflicti533 – 5331A → T in AAA34840. (PubMed:8346682)Curated
    Sequence conflicti586 – 5861D → Y in AAB49319. (PubMed:8725220)Curated
    Sequence conflicti590 – 5912CE → FV in AAA34840. (PubMed:8346682)Curated
    Sequence conflicti721 – 7211G → GL in AAB49319. (PubMed:8725220)Curated
    Sequence conflicti722 – 7221V → G in AAA34840. (PubMed:8346682)Curated
    Sequence conflicti735 – 7373NGD → FGF in AAA34840. (PubMed:8346682)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15299 Genomic DNA. Translation: AAA34840.1. Frameshift.
    U43608 Genomic DNA. Translation: AAB49319.1. Frameshift.
    Z71648 Genomic DNA. Translation: CAA96313.1.
    AY693029 Genomic DNA. Translation: AAT93048.1.
    BK006947 Genomic DNA. Translation: DAA10574.1.
    PIRiS63364.
    RefSeqiNP_014431.1. NM_001183210.1.

    Genome annotation databases

    EnsemblFungiiYNR033W; YNR033W; YNR033W.
    GeneIDi855768.
    KEGGisce:YNR033W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15299 Genomic DNA. Translation: AAA34840.1 . Frameshift.
    U43608 Genomic DNA. Translation: AAB49319.1 . Frameshift.
    Z71648 Genomic DNA. Translation: CAA96313.1 .
    AY693029 Genomic DNA. Translation: AAT93048.1 .
    BK006947 Genomic DNA. Translation: DAA10574.1 .
    PIRi S63364.
    RefSeqi NP_014431.1. NM_001183210.1.

    3D structure databases

    ProteinModelPortali P37254.
    SMRi P37254. Positions 17-224, 492-786.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35858. 47 interactions.
    DIPi DIP-6278N.
    IntActi P37254. 9 interactions.
    MINTi MINT-604611.

    Chemistry

    DrugBanki DB00259. Sulfanilamide.

    Protein family/group databases

    MEROPSi C26.958.

    Proteomic databases

    MaxQBi P37254.
    PaxDbi P37254.
    PeptideAtlasi P37254.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR033W ; YNR033W ; YNR033W .
    GeneIDi 855768.
    KEGGi sce:YNR033W.

    Organism-specific databases

    CYGDi YNR033w.
    SGDi S000005316. ABZ1.

    Phylogenomic databases

    eggNOGi COG0147.
    GeneTreei ENSGT00620000088738.
    HOGENOMi HOG000025143.
    KOi K13950.
    OMAi YEANDAT.
    OrthoDBi EOG7PZS5V.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00149 .
    BioCyci YEAST:YNR033W-MONOMER.

    Miscellaneous databases

    NextBioi 980214.

    Gene expression databases

    Genevestigatori P37254.

    Family and domain databases

    Gene3Di 3.40.50.880. 1 hit.
    3.60.120.10. 1 hit.
    InterProi IPR005801. ADC_synthase.
    IPR006805. Anth_synth_I_N.
    IPR015890. Chorismate-bd_C.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010117. PabB_fungal.
    IPR006221. TrpG/PapA_dom.
    [Graphical view ]
    Pfami PF04715. Anth_synt_I_N. 1 hit.
    PF00425. Chorismate_bind. 1 hit.
    PF00117. GATase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    SSF56322. SSF56322. 1 hit.
    TIGRFAMsi TIGR01823. PabB-fungal. 1 hit.
    TIGR00566. trpG_papA. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Para-aminobenzoate synthase gene of Saccharomyces cerevisiae encodes a bifunctional enzyme."
      Edman J.C., Goldstein A.L., Erbe J.G.
      Yeast 9:669-675(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "Los1p, involved in yeast pre-tRNA splicing, positively regulates members of the SOL gene family."
      Shen W.-C., Stanford D.R., Hopper A.K.
      Genetics 143:699-712(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPABS_YEAST
    AccessioniPrimary (citable) accession number: P37254
    Secondary accession number(s): D6W1K8, Q02982, Q6B1Q1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: February 21, 2006
    Last modified: October 1, 2014
    This is version 146 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1550 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3