P37237 (FGF8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fibroblast growth factor 8
Short name=FGF-8

Alternative name(s):
Androgen-induced growth factor
Short name=AIGF
Heparin-binding growth factor 8
Short name=HBGF-8

Gene names

Name:	Fgf8
Synonyms:	Aigf

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system By similarity. Cooperates with Wnt-1 in mouse mammary tumor virus-induced murine mammary tumorigenesis.
Subunit structure	Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors By similarity.
Subcellular location	Secreted.
Tissue specificity	Absent in normal mammary glands and detected only in adult testis and ovary and in midgestational embryos.
Induction	By androgens.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the heparin-binding growth factors family.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Developmental protein Growth factor Mitogen
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from direct assay PubMed 17360443. Source: MGI MAPK cascade Inferred from mutant phenotype PubMed 16720880. Source: MGI aorta morphogenesis Inferred from genetic interaction PubMed 21364285. Source: MGI apoptotic process Inferred from mutant phenotype PubMed 16613831. Source: MGI blood vessel remodeling Inferred from genetic interaction PubMed 17000704. Source: MGI bone development Inferred from electronic annotation. Source: Compara branching involved in salivary gland morphogenesis Inferred from mutant phenotype PubMed 15063181. Source: MGI branching involved in ureteric bud morphogenesis Inferred from mutant phenotype PubMed 16049111. Source: MGI canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 17360443. Source: MGI cell migration involved in mesendoderm migration Inferred from mutant phenotype PubMed 10421635. Source: MGI cell proliferation in forebrain Inferred from mutant phenotype PubMed 16613831. Source: MGI corticotropin hormone secreting cell differentiation Inferred from direct assay PubMed 9463347. Source: MGI dopaminergic neuron differentiation Inferred from electronic annotation. Source: Compara embryonic hindlimb morphogenesis Inferred from genetic interaction PubMed 16245339. Source: MGI epithelial to mesenchymal transition involved in endocardial cushion formation Inferred from mutant phenotype PubMed 19509466. Source: BHF-UCL fibroblast growth factor receptor signaling pathway Inferred from direct assay PubMed 10381577PubMed 9435295. Source: MGI forebrain dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 16613831. Source: MGI forebrain morphogenesis Inferred from mutant phenotype PubMed 16613831. Source: MGI generation of neurons Inferred from mutant phenotype PubMed 16267092. Source: MGI gonad development Inferred from electronic annotation. Source: Compara heart looping Inferred from mutant phenotype PubMed 10411502. Source: MGI kidney development Inferred from mutant phenotype PubMed 16049111. Source: MGI lung morphogenesis Inferred from mutant phenotype PubMed 10411502. Source: MGI male genitalia development Inferred from direct assay PubMed 12783783. Source: MGI mesodermal cell migration Inferred from mutant phenotype PubMed 10421635. Source: MGI mesonephros development Inferred from electronic annotation. Source: Compara metanephros development Inferred from electronic annotation. Source: Compara midbrain-hindbrain boundary development Inferred from mutant phenotype PubMed 17601531. Source: MGI negative regulation of cardiac muscle tissue development Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 16245339. Source: MGI neural plate morphogenesis Inferred from mutant phenotype PubMed 16613831. Source: MGI neuroepithelial cell differentiation Inferred from electronic annotation. Source: Compara odontogenesis Inferred from electronic annotation. Source: Compara organ induction Inferred from genetic interaction PubMed 15741321. Source: MGI otic vesicle formation Inferred from genetic interaction PubMed 17601531. Source: MGI outflow tract septum morphogenesis Inferred from genetic interaction PubMed 20807544. Source: UniProtKB pallium development Inferred from mutant phenotype PubMed 16613831. Source: MGI patterning of blood vessels Inferred from genetic interaction PubMed 15996652. Source: MGI pharyngeal system development Inferred from genetic interaction PubMed 21364285. Source: MGI positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell proliferation Inferred from direct assay PubMed 10381577PubMed 9463347. Source: MGI positive regulation of gene expression Inferred from direct assay PubMed 9244299PubMed 9847236. Source: MGI positive regulation of mitosis Inferred from direct assay PubMed 12783783. Source: MGI positive regulation of organ growth Inferred from mutant phenotype PubMed 19389367. Source: MGI regulation of odontogenesis of dentin-containing tooth Inferred from genetic interaction PubMed 9244299. Source: MGI signal transduction involved in regulation of gene expression Inferred from direct assay PubMed 18462699. Source: MGI subpallium development Inferred from mutant phenotype PubMed 16613831. Source: MGI thyroid gland development Inferred from mutant phenotype PubMed 19389367. Source: MGI thyroid-stimulating hormone-secreting cell differentiation Inferred from direct assay PubMed 9463347. Source: MGI
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform FGF-8C (identifier: P37237-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform FGF-8B (identifier: P37237-2)

The sequence of this isoform differs from the canonical sequence as follows:
     24-87: VRSAAQKRGPGAGNPADTLGQGHEDRPFGQRSRAGKNFTNPAPNYPEEGSKEQRDSVLPKVTQR → VTVQSSPNFTQ

Isoform FGF-8A (identifier: P37237-3)

The sequence of this isoform differs from the canonical sequence as follows:
     24-87: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 268	246	Fibroblast growth factor 8		PRO_0000008971
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid Potential
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	24 – 87	64	Missing in isoform FGF-8A.		VSP_001528
	Alternative sequence	24 – 87	64	VRSAA…KVTQR → VTVQSSPNFTQ in isoform FGF-8B.		VSP_001527

Sequences

	Sequence		Length	Mass (Da)
	Isoform FGF-8C [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 3330A9F342AD7109	FASTA	268	30,420
	10 20 30 40 50 60 MGSPRSALSC LLLHLLVLCL QAQVRSAAQK RGPGAGNPAD TLGQGHEDRP FGQRSRAGKN 70 80 90 100 110 120 FTNPAPNYPE EGSKEQRDSV LPKVTQRHVR EQSLVTDQLS RRLIRTYQLY SRTSGKHVQV 130 140 150 160 170 180 LANKRINAMA EDGDPFAKLI VETDTFGSRV RVRGAETGLY ICMNKKGKLI AKSNGKGKDC 190 200 210 220 230 240 VFTEIVLENN YTALQNAKYE GWYMAFTRKG RPRKGSKTRQ HQREVHFMKR LPRGHHTTEQ 250 260 SLRFEFLNYP PFTRSLRGSQ RTWAPEPR
	Isoform FGF-8B [UniParc]. Checksum: A39424271EF7CBFF	FASTA	215	24,711
	10 20 30 40 50 60 MGSPRSALSC LLLHLLVLCL QAQVTVQSSP NFTQHVREQS LVTDQLSRRL IRTYQLYSRT 70 80 90 100 110 120 SGKHVQVLAN KRINAMAEDG DPFAKLIVET DTFGSRVRVR GAETGLYICM NKKGKLIAKS 130 140 150 160 170 180 NGKGKDCVFT EIVLENNYTA LQNAKYEGWY MAFTRKGRPR KGSKTRQHQR EVHFMKRLPR 190 200 210 GHHTTEQSLR FEFLNYPPFT RSLRGSQRTW APEPR
	Isoform FGF-8A [UniParc]. Checksum: 9A4CAB7686A2B190	FASTA	204	23,522
	10 20 30 40 50 60 MGSPRSALSC LLLHLLVLCL QAQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK 70 80 90 100 110 120 RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE 130 140 150 160 170 180 IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF 190 200 EFLNYPPFTR SLRGSQRTWA PEPR

References

[1]	"Cloning and characterization of an androgen-induced growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells." Tanaka A., Miyamoto K., Minamino N., Takeda M., Sato B., Matsuo H., Matsumoto K. Proc. Natl. Acad. Sci. U.S.A. 89:8928-8932(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Fgf-8, activated by proviral insertion, cooperates with the Wnt-1 transgene in murine mammary tumorigenesis." Macarthur C.A., Shankar D.B., Shackleford G.M. J. Virol. 69:2501-2507(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary carcinoma.
[3]	"A role for FGF-8 in the initiation and maintenance of vertebrate limb bud outgrowth." Mahmood R., Bresnick J., Hornbruch A., Mahony C., Morton N., Colquhoun K., Martin P., Lumsden A., Dickson C., Mason I. Curr. Biol. 5:797-806(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12483 mRNA. Translation: BAA02051.1. D12482 mRNA. Translation: BAA02050.1. U18673 mRNA. Translation: AAA65387.1. Z48746 mRNA. Translation: CAA88637.1.
IPI	IPI00125192. IPI00230745. IPI00230746.
PIR	A46245. B46245.
RefSeq	NP_001159834.1. NM_001166362.1. NP_001159835.1. NM_001166363.1. NP_034335.1. NM_010205.2.
UniGene	Mm.4012.
3D structure databases
ProteinModelPortal	P37237.
SMR	P37237. Positions 87-233.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6030N.
STRING	10090.ENSMUSP00000026241.
PTM databases
PhosphoSite	P37237.
Proteomic databases
PRIDE	P37237.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026241; ENSMUSP00000026241; ENSMUSG00000025219. ENSMUST00000111927; ENSMUSP00000107558; ENSMUSG00000025219. ENSMUST00000111928; ENSMUSP00000107559; ENSMUSG00000025219.
GeneID	14179.
KEGG	mmu:14179.
UCSC	uc008hrh.2. mouse. uc012bmo.1. mouse.
Organism-specific databases
CTD	2253.
MGI	MGI:99604. Fgf8.
Phylogenomic databases
eggNOG	NOG327940.
GeneTree	ENSGT00690000101834.
HOGENOM	HOG000115986.
HOVERGEN	HBG005659.
InParanoid	P37237.
KO	K04358.
Gene expression databases
ArrayExpress	P37237.
Bgee	P37237.
CleanEx	MM_FGF8.
Genevestigator	P37237.
GermOnline	ENSMUSG00000025219. Mus musculus.
Family and domain databases
InterPro	IPR008996. Cytokine_IL1-like. IPR002348. IL1_HBGF. [Graphical view]
PANTHER	PTHR11486. PTHR11486. 1 hit.
Pfam	PF00167. FGF. 1 hit. [Graphical view]
PRINTS	PR00262. IL1HBGF.
SMART	SM00442. FGF. 1 hit. [Graphical view]
SUPFAM	SSF50353. Cytok_IL1_like. 1 hit.
PROSITE	PS00247. HBGF_FGF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	285366.
SOURCE	Search...

Entry information

Entry name

FGF8_MOUSE

Accession

Primary (citable) accession number: P37237

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program