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P37228 (MDHG_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase, glyoxysomal

EC=1.1.1.37
OrganismGlycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3333Glyoxysome Potential
Chain34 – 353320Malate dehydrogenase, glyoxysomal
PRO_0000018641

Regions

Nucleotide binding48 – 547NAD By similarity
Nucleotide binding157 – 1593NAD By similarity

Sites

Active site2171Proton acceptor By similarity
Binding site741NAD By similarity
Binding site1211Substrate By similarity
Binding site1271Substrate By similarity
Binding site1341NAD By similarity
Binding site1591Substrate By similarity
Binding site1931Substrate By similarity
Binding site2681NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
P37228 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: D2B0BF1AE6CC8925

FASTA35337,395
        10         20         30         40         50         60 
MEANSGASDR ISRIAGHLRP QREDDVCLKR SDCRAKGGVS GFKVAILGAA GGIGQPLAML 

        70         80         90        100        110        120 
MKMNPLVSLL HLYDVVNTPG VTSDISHMDT GAVVRGFLGQ QQLEDALIGM DLVIIPAGVP 

       130        140        150        160        170        180 
RKPGMTRDDL FNINAGIVKT LCEAIAKCCP KAIVNVISNP VNSTVPIAAE VFKRAGTYDP 

       190        200        210        220        230        240 
KRLLGVTMLD VVRANTFVAE VLGVDPRDVD VPVVGGHAGI TILPLLSQIK PPCSFTPKEI 

       250        260        270        280        290        300 
EYLTGRIQNG GPEVVEAKAG AGSATLSMAY AAVKFADACL HALRGDAGII ECAYVASQVT 

       310        320        330        340        350 
ELPFFASKVR LGRVGVEEIL PLGPLNDYER ESLEKAKKEL AASIEKGISF IRK 

« Hide

References

[1]"Glyoxysomal malate dehydrogenase and malate synthase from soybean cotyledons (Glycine max L.): enzyme association, antibody production and cDNA cloning."
Guex N., Henry H., Flach J., Richter H., Widmer F.
Planta 197:369-375(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-353.
Strain: cv. Maple Arrow.
Tissue: Cotyledon.
[2]Richter H., Guex N.
Submitted (JAN-1996) to UniProtKB
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-3.
Strain: cv. Maple Arrow.
Tissue: Cotyledon.
[3]"3D modeling of soybean glyoxysomal malate dehydrogenase (gMDH)."
Guex N., Widmer F., Gaillard P., Carrupt P.-A.
(In) Wermuth C.G. (eds.); Trends in QSAR and molecular modelling 92, pp.485-486, Escom Science Publishers, Leiden (1993)
Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L01628 mRNA. Translation: AAC37464.1.
UniGeneGma.1535.

3D structure databases

ProteinModelPortalP37228.
SMRP37228. Positions 41-352.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP37228.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Gene expression databases

GenevestigatorP37228.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PTHR11540:SF1. PTHR11540:SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHG_SOYBN
AccessionPrimary (citable) accession number: P37228
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families