Reviewed, UniProtKB/Swiss-Prot P37228 (MDHG_SOYBN)

Names and origin

Protein names	Recommended name:     Malate dehydrogenase, glyoxysomal     EC=1.1.1.37
Organism	Glycine max (Soybean)
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Glyoxysome Potential
	Chain	34 – 353	320	Malate dehydrogenase, glyoxysomal		PRO_0000018641
Regions
	Nucleotide binding	48 – 54	7	NAD By similarity
	Nucleotide binding	157 – 159	3	NAD By similarity
Sites
	Active site	217	1	Proton acceptor By similarity
	Binding site	74	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	127	1	Substrate By similarity
	Binding site	134	1	NAD By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	193	1	Substrate By similarity
	Binding site	268	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37228-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: D2B0BF1AE6CC8925	FASTA	353	37,395
	10 20 30 40 50 60 MEANSGASDR ISRIAGHLRP QREDDVCLKR SDCRAKGGVS GFKVAILGAA GGIGQPLAML 70 80 90 100 110 120 MKMNPLVSLL HLYDVVNTPG VTSDISHMDT GAVVRGFLGQ QQLEDALIGM DLVIIPAGVP 130 140 150 160 170 180 RKPGMTRDDL FNINAGIVKT LCEAIAKCCP KAIVNVISNP VNSTVPIAAE VFKRAGTYDP 190 200 210 220 230 240 KRLLGVTMLD VVRANTFVAE VLGVDPRDVD VPVVGGHAGI TILPLLSQIK PPCSFTPKEI 250 260 270 280 290 300 EYLTGRIQNG GPEVVEAKAG AGSATLSMAY AAVKFADACL HALRGDAGII ECAYVASQVT 310 320 330 340 350 ELPFFASKVR LGRVGVEEIL PLGPLNDYER ESLEKAKKEL AASIEKGISF IRK

References

[1]	"Glyoxysomal malate dehydrogenase and malate synthase from soybean cotyledons (Glycine max L.): enzyme association, antibody production and cDNA cloning." Guex N., Henry H., Flach J., Richter H., Widmer F. Planta 197:369-375(1995) [PubMed: 8547819] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-353. Strain: cv. Maple Arrow. Tissue: Cotyledon.
[2]	Richter H., Guex N. Submitted (JAN-1996) to UniProtKB Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-3. Strain: cv. Maple Arrow. Tissue: Cotyledon.
[3]	"3D modeling of soybean glyoxysomal malate dehydrogenase (gMDH)." Guex N., Widmer F., Gaillard P., Carrupt P.-A. (In) Wermuth C.G. (eds.); Trends in QSAR and molecular modelling 92, pp.485-486, Escom Science Publishers, Leiden (1993) Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L01628 mRNA. Translation: AAC37464.1.
UniGene	Gma.1535
3D structure databases
HSSP	HSSP built from PDB template 1MLD based on UniProtKB P00346.
SMR	P37228. Positions 41-352.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.37. 299.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHG_SOYBN

Accession

Primary (citable) accession number: P37228

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)