Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate dehydrogenase, glyoxysomal

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741NADBy similarity
Binding sitei121 – 1211SubstratePROSITE-ProRule annotation
Binding sitei127 – 1271SubstratePROSITE-ProRule annotation
Binding sitei134 – 1341NADBy similarity
Binding sitei159 – 1591SubstratePROSITE-ProRule annotation
Binding sitei193 – 1931SubstratePROSITE-ProRule annotation
Active sitei217 – 2171Proton acceptorBy similarity
Binding sitei268 – 2681NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi48 – 547NADBy similarity
Nucleotide bindingi157 – 1593NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333GlyoxysomeSequence AnalysisAdd
BLAST
Chaini34 – 353320Malate dehydrogenase, glyoxysomalPRO_0000018641Add
BLAST

Proteomic databases

PRIDEiP37228.

Expressioni

Gene expression databases

GenevestigatoriP37228.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP37228.
SMRiP37228. Positions 41-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEANSGASDR ISRIAGHLRP QREDDVCLKR SDCRAKGGVS GFKVAILGAA
60 70 80 90 100
GGIGQPLAML MKMNPLVSLL HLYDVVNTPG VTSDISHMDT GAVVRGFLGQ
110 120 130 140 150
QQLEDALIGM DLVIIPAGVP RKPGMTRDDL FNINAGIVKT LCEAIAKCCP
160 170 180 190 200
KAIVNVISNP VNSTVPIAAE VFKRAGTYDP KRLLGVTMLD VVRANTFVAE
210 220 230 240 250
VLGVDPRDVD VPVVGGHAGI TILPLLSQIK PPCSFTPKEI EYLTGRIQNG
260 270 280 290 300
GPEVVEAKAG AGSATLSMAY AAVKFADACL HALRGDAGII ECAYVASQVT
310 320 330 340 350
ELPFFASKVR LGRVGVEEIL PLGPLNDYER ESLEKAKKEL AASIEKGISF

IRK
Length:353
Mass (Da):37,395
Last modified:February 1, 1996 - v2
Checksum:iD2B0BF1AE6CC8925
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01628 mRNA. Translation: AAC37464.1.
UniGeneiGma.1535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01628 mRNA. Translation: AAC37464.1.
UniGeneiGma.1535.

3D structure databases

ProteinModelPortaliP37228.
SMRiP37228. Positions 41-352.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP37228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Gene expression databases

GenevestigatoriP37228.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Glyoxysomal malate dehydrogenase and malate synthase from soybean cotyledons (Glycine max L.): enzyme association, antibody production and cDNA cloning."
    Guex N., Henry H., Flach J., Richter H., Widmer F.
    Planta 197:369-375(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-353.
    Strain: cv. Maple Arrow.
    Tissue: Cotyledon.
  2. Richter H., Guex N.
    Submitted (JAN-1996) to UniProtKB
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-3.
    Strain: cv. Maple Arrow.
    Tissue: Cotyledon.
  3. "3D modeling of soybean glyoxysomal malate dehydrogenase (gMDH)."
    Guex N., Widmer F., Gaillard P., Carrupt P.-A.
    (In) Wermuth C.G. (eds.); Trends in QSAR and molecular modelling 92, pp.485-486, Escom Science Publishers, Leiden (1993)
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiMDHG_SOYBN
AccessioniPrimary (citable) accession number: P37228
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.