Reviewed, UniProtKB/Swiss-Prot P37225 (MAON_SOLTU)

Names and origin

Protein names	Recommended name:     NAD-dependent malic enzyme 59 kDa isoform, mitochondrial       Short name=NAD-ME     EC=1.1.1.39
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Heterodimer of two related subunits.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 18	18	Mitochondrion
	Chain	19 – 601	583	NAD-dependent malic enzyme 59 kDa isoform, mitochondrial		PRO_0000018544
Sites
	Active site	129	1	Proton donor By similarity
	Active site	200	1	Proton acceptor By similarity
	Metal binding	271	1	Divalent metal cation By similarity
	Metal binding	272	1	Divalent metal cation By similarity
	Metal binding	295	1	Divalent metal cation By similarity
	Binding site	182	1	NAD By similarity
	Binding site	295	1	NAD By similarity
	Binding site	444	1	NAD By similarity
	Site	295	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37225-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: D6C2A128CC9E971C	FASTA	601	66,272
	10 20 30 40 50 60 MWRVARSAAS TFRRTRRLST AISAPCIVHK RGADILHDPW FNKDTGFPMT ERDRLGLRGL 70 80 90 100 110 120 LPPRVISFEQ QYDRFMESFR SLEKNTEGQP DSVVSLAKWR ILNRLHDRNE TLYYRVLIDN 130 140 150 160 170 180 IKDFAPIIYT PTVGLVCQNY SGLFRRPRGM YFSAKDKGEM MSMIFNWPST QVDMIVLTDG 190 200 210 220 230 240 SRILGLGDLG VQGIGIPIGK LDMYVAAAGI NPQRVLPVML DVGTNNQKLL EDPLYLGLRQ 250 260 270 280 290 300 PRLEGEEYLS IVDEFVEAVH ARWPKAVVQF EDFQAKWAFE TLDRYRKKFC MFNDDIQGTA 310 320 330 340 350 360 GVALAGLLGT VRAQGRPLTD FANQKIVVVG AGSAGLGVLK MALQAVSRMT GPSADPHFFL 370 380 390 400 410 420 LDKNGLITKD RKDIDPAALP FAKAHHEIEG LGLQEGAGLA EVVKKVKPHV LLGLSGVGGI 430 440 450 460 470 480 FHEEVLRAMK ESDSVRPAIF AMSNPTNNAE CCPVDAFKLA GEDIVFASGS PFANVDLGNG 490 500 510 520 530 540 KIGHVNQANN MYLFPGIGLG ALLSGARNIS DTMLEAAAEC LASYMSDDEI NRGILYPSID 550 560 570 580 590 600 DIRDITAEVG AAVLRAAVAE DLAEGHGDVG VKELQHMSKE ETIEHVRQNM WYPVYGPLVH E

References

[1]

"Plant mitochondrial NAD+-dependent malic enzyme. cDNA cloning, deduced primary structure of the 59- and 62-kDa subunits, import, gene complexity and expression analysis." Winning B.M., Bourguignon J., Leaver C.J. J. Biol. Chem. 269:4780-4786(1994) [PubMed: 8106447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Desiree. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z23002 mRNA. Translation: CAA80547.1.
PIR	A53318.
3D structure databases
HSSP	HSSP built from PDB template 1LLQ based on UniProtKB P27443.
ModBase	Search...
Protein-protein interaction databases
IntAct	P37225.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAON_SOLTU

Accession

Primary (citable) accession number: P37225

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)