Reviewed, UniProtKB/Swiss-Prot P37224 (MAOM_AMAHP)

Names and origin

Protein names	Recommended name:     NAD-dependent malic enzyme 65 kDa isoform, mitochondrial       Short name=NAD-ME     EC=1.1.1.39
Organism	Amaranthus hypochondriacus (Prince-of-Wales feather)
Taxonomic identifier	28502 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Amaranthus

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Pathway	Photosynthesis; C4 acid pathway.
Subunit structure	Heterodimer of two related subunits.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 31	31	Mitochondrion
	Chain	32 – 623	592	NAD-dependent malic enzyme 65 kDa isoform, mitochondrial		PRO_0000018542
Sites
	Active site	143	1	Proton donor Potential
	Active site	214	1	Proton acceptor By similarity
	Metal binding	285	1	Divalent metal cation By similarity
	Metal binding	286	1	Divalent metal cation By similarity
	Metal binding	309	1	Divalent metal cation By similarity
	Binding site	196	1	NAD By similarity
	Binding site	309	1	NAD By similarity
	Binding site	464	1	NAD By similarity
	Site	309	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37224-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 0B0917B8506FFE48	FASTA	623	69,688
	10 20 30 40 50 60 MLVLCSRSRL TSSLIRRLKD QIANVSNHRS FATSEGHRLA IVNKRSLDIL QDPWFNKGTA 70 80 90 100 110 120 FSMTERDRLD LRGLLPPNVM TTEQQIERFT ADLRVLELTT KDGPSDTYDL AKWRILNRLH 130 140 150 160 170 180 DRNETMFFKV LIENIEEYAP IVSTPTVGLV CQKFSGLYRR PRGMYFSSDD RGEMMSMVYN 190 200 210 220 230 240 WPAEQVDMIV VTDGSRILGL GDLGVHGIGV AIGKLDLYVA AAGINPQRVL PVMIDVGTNN 250 260 270 280 290 300 EDLLKNPLYL GLQKKRLDGE EYLAVMDEFM EAVFTRWPNV IVQFEDIQNK WALTLLQRYR 310 320 330 340 350 360 HKYRTFNVDV QGTSGVAIAG LLGAVRAQGR PMIDFPKQKI VVAGAGSSGV GVLNAARKTM 370 380 390 400 410 420 ARMLGNDESA FDRARSQFWV VDDKGLITEK RANLDPEVQP FAWKENEISL QGLNEGAKLV 430 440 450 460 470 480 EVVRQVKPDV LLGLSAYGGL FSKEVLEALK DSTSTRPAIF AMSNPTKNAE CTPEEAFSIV 490 500 510 520 530 540 GDHVVYASGS PFKDVDLGNG KIGHVNQGNN MYLFPGIGLG VLLSGSRIIS DSMFQAAAER 550 560 570 580 590 600 LAGYMTDEEV INGVIYPSIS RIRDITKEVA AAVIKEAVEE DLAEGYRDMD ARELQKLNEE 610 620 QILEYIEKNM WNPEYPTLVY KKR

References

[1]	"Cloning and analysis of the C4 photosynthetic NAD-dependent malic enzyme of amaranth mitochondria." Long J.J., Wang J.-L., Berry J.O. J. Biol. Chem. 269:2827-2833(1994) [PubMed: 8300616] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01162 mRNA. Translation: AAA19014.1.
PIR	A49983.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.39. 2722.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOM_AMAHP

Accession

Primary (citable) accession number: P37224

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)