ID   MAOX_MESCR              Reviewed;         585 AA.
AC   P37223;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   16-JUN-2009, entry version 59.
DE   RecName: Full=NADP-dependent malic enzyme;
DE            Short=NADP-ME;
DE            EC=1.1.1.40;
GN   Name=MOD1;
OS   Mesembryanthemum crystallinum (Common ice plant).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   Caryophyllales; Aizoaceae; Mesembryanthemum.
OX   NCBI_TaxID=3544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   MEDLINE=92394118; PubMed=1521524;
RX   DOI=10.1111/j.1432-1033.1992.tb17181.x;
RA   Cushman J.C.;
RT   "Characterization and expression of a NADP-malic enzyme cDNA induced
RT   by salt stress from the facultative crassulacean acid metabolism
RT   plant, Mesembryanthemum crystallinum.";
RL   Eur. J. Biochem. 208:259-266(1992).
CC   -!- FUNCTION: Plays a role in CAM (crassulacean acid metabolism)
CC       photosynthesis.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NADP(+) = pyruvate + CO(2) +
CC       NADPH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- TISSUE SPECIFICITY: Leaves.
CC   -!- INDUCTION: By salt stress.
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; X64434; CAA45772.1; -; mRNA.
DR   PIR; S43718; S43718.
DR   HSSP; P40927; 1GQ2.
DR   BRENDA; 1.1.1.40; 2210.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004473; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006950; P:response to stress; IEA:UniProtKB-KW.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Metal-binding; NADP; Oxidoreductase; Stress response.
FT   CHAIN         1    585       NADP-dependent malic enzyme.
FT                                /FTId=PRO_0000160200.
FT   NP_BIND     329    345       NADP (By similarity).
FT   ACT_SITE    133    133       Proton donor (By similarity).
FT   ACT_SITE    204    204       Proton acceptor (By similarity).
FT   METAL       276    276       Divalent metal cation (By similarity).
FT   METAL       277    277       Divalent metal cation (By similarity).
FT   METAL       300    300       Divalent metal cation (By similarity).
FT   BINDING     186    186       NAD (By similarity).
FT   BINDING     300    300       NAD (By similarity).
FT   BINDING     441    441       NAD (By similarity).
FT   SITE        300    300       Important for activity (By similarity).
SQ   SEQUENCE   585 AA;  64358 MW;  C71675B67FB719D9 CRC64;
     MGGSNALNEM TNGSDGITGG VADVYGEEFA TQDQLVTPWS FSVACGHSLL RDPQHNKGLA
     FTEKERDAHF LRGLLPPVVL SQELQEKKFL TTLRQYQVPL QKYMAMMDLQ ERNEKLFYKL
     LVDHVEELLP LVYTPTVGEG CQKYGSIFRR PQGLFISLKD KGRILELLRN WPEKKIQVIV
     VTDGERILGL GDLGCQGMGI PVGKLSLYSA LGGVCPSACL PITLDVGTNN QKLLDDEFYI
     GLKQKRATGE EYAEFVQEFM SAVKQNYGEK ILVQFEDFAN HNAFELLEKY RTTHLVFNDD
     IQGTASVVLA GLIASLKLLG GTLADHKFLF LGAGEAGTGI AELIALEMSK KTKAPVEQMR
     KKIWLVDSKG LVVSSRKETL QQFKLPWAHE HEPITTLIDA VQAIKPTVLI GTSGKGKQFT
     KEVVEAMANI NAKPLILALS NPTSQSECTA EEAYTWSQGH AIFASGSPFD PVEYEGRTFV
     PGQANNAYIF PGFGLGLIMC GAIRVHDDML LAASEALASQ VTGEHFIKGL IYPPFKDIRK
     ISAHIAAGVA AKAYELGLAS RLPQPADLVK FAESCMYNPT YRSFR
//