Reviewed, UniProtKB/Swiss-Prot P37223 (MAOX_MESCR)

Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40

Gene names

Name:

MOD1

Organism

Mesembryanthemum crystallinum (Common ice plant)

Taxonomic identifier

3544 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Aizoaceae › Mesembryanthemum

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in CAM (crassulacean acid metabolism) photosynthesis.
Catalytic activity	(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Leaves.
Induction	By salt stress.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	NADP-dependent malic enzyme		PRO_0000160200
Regions
	Nucleotide binding	329 – 345	17	NADP By similarity
Sites
	Active site	133	1	Proton donor By similarity
	Active site	204	1	Proton acceptor By similarity
	Metal binding	276	1	Divalent metal cation By similarity
	Metal binding	277	1	Divalent metal cation By similarity
	Metal binding	300	1	Divalent metal cation By similarity
	Binding site	186	1	NAD By similarity
	Binding site	300	1	NAD By similarity
	Binding site	441	1	NAD By similarity
	Site	300	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37223-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: C71675B67FB719D9	FASTA	585	64,358
	10 20 30 40 50 60 MGGSNALNEM TNGSDGITGG VADVYGEEFA TQDQLVTPWS FSVACGHSLL RDPQHNKGLA 70 80 90 100 110 120 FTEKERDAHF LRGLLPPVVL SQELQEKKFL TTLRQYQVPL QKYMAMMDLQ ERNEKLFYKL 130 140 150 160 170 180 LVDHVEELLP LVYTPTVGEG CQKYGSIFRR PQGLFISLKD KGRILELLRN WPEKKIQVIV 190 200 210 220 230 240 VTDGERILGL GDLGCQGMGI PVGKLSLYSA LGGVCPSACL PITLDVGTNN QKLLDDEFYI 250 260 270 280 290 300 GLKQKRATGE EYAEFVQEFM SAVKQNYGEK ILVQFEDFAN HNAFELLEKY RTTHLVFNDD 310 320 330 340 350 360 IQGTASVVLA GLIASLKLLG GTLADHKFLF LGAGEAGTGI AELIALEMSK KTKAPVEQMR 370 380 390 400 410 420 KKIWLVDSKG LVVSSRKETL QQFKLPWAHE HEPITTLIDA VQAIKPTVLI GTSGKGKQFT 430 440 450 460 470 480 KEVVEAMANI NAKPLILALS NPTSQSECTA EEAYTWSQGH AIFASGSPFD PVEYEGRTFV 490 500 510 520 530 540 PGQANNAYIF PGFGLGLIMC GAIRVHDDML LAASEALASQ VTGEHFIKGL IYPPFKDIRK 550 560 570 580 ISAHIAAGVA AKAYELGLAS RLPQPADLVK FAESCMYNPT YRSFR

References

[1]

"Characterization and expression of a NADP-malic enzyme cDNA induced by salt stress from the facultative crassulacean acid metabolism plant, Mesembryanthemum crystallinum." Cushman J.C. Eur. J. Biochem. 208:259-266(1992) [PubMed: 1521524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64434 mRNA. Translation: CAA45772.1.
PIR	S43718.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.40. 2210.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOX_MESCR

Accession

Primary (citable) accession number: P37223

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)