ID   MAOC_SOLLC              Reviewed;         573 AA.
AC   P37222;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=NADP-dependent malic enzyme, chloroplastic;
DE            Short=NADP-ME;
DE            EC=1.1.1.40;
DE   Flags: Fragment;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae;
OC   Solanum; Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Rutgers;
RA   Finger F.L., Knee M., Lagrimini L.M.A.;
RT   "Isolation of a cDNA clone for tomato fruit NADP+ malic enzyme.";
RL   Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The chloroplastic ME isoform decarboxylates malate
CC       shuttled from neighboring mesophyll cells. The CO(2) released is
CC       then refixed by ribulose-bisphosphate carboxylase. This pathway
CC       eliminates the photorespiratory loss of CO(2) that occurs in most
CC       plants.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NADP(+) = pyruvate + CO(2) +
CC       NADPH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- PATHWAY: Photosynthesis; C4 acid pathway.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; L27509; AAA34174.1; -; mRNA.
DR   PIR; T07088; T07088.
DR   HSSP; P40927; 1GQ2.
DR   BRENDA; 1.1.1.40; 281054.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004473; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Metal-binding; NAD; NADP; Oxidoreductase; Plastid.
FT   CHAIN        <1    573       NADP-dependent malic enzyme,
FT                                chloroplastic.
FT                                /FTId=PRO_0000160199.
FT   NP_BIND     318    334       NADP (By similarity).
FT   ACT_SITE    123    123       Proton donor (By similarity).
FT   ACT_SITE    194    194       Proton acceptor (By similarity).
FT   METAL       265    265       Divalent metal cation (By similarity).
FT   METAL       266    266       Divalent metal cation (By similarity).
FT   METAL       289    289       Divalent metal cation (By similarity).
FT   BINDING     176    176       NAD (By similarity).
FT   BINDING     289    289       NAD (By similarity).
FT   BINDING     430    430       NAD (By similarity).
FT   SITE        289    289       Important for activity (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   573 AA;  63561 MW;  13846E61206F8E75 CRC64;
     IRHESTVTGG VQDVYGEDSA TEDQSITPWT LSVASGFSLL RNPHYNKGLA FSERERDTHY
     LRGLLPPVVI SHDLQVKKMM NSIRKYDVPL QRYMAMMDLQ EMNERLFYKL LIDNVEELLP
     IVYTPTVGEA CQKYGWIFKR PQGLFFSLKE KGKIHEVLKN WPEKKIQVIV VTDGERILGL
     GDLGCQGMGI PVGKLSLYSA LGGIRPSACL PVTIDVGQTM KFVDDEFYIG LRQRRATGQE
     YSELLDEFMY AVKQNYGEKV LIQFEDFANH NAFNLLAKYG TSHLVFNDDI QGTASVVLAG
     LMAALNLVGG SLSEHTFLFL GAGEAGTGIA ELIALEMSKQ TGIPLEETRK KIWMVDSKGL
     IVKSRMEMLQ HFKRPWAHDH EPVQELVNAV KSIKPTVLIG SSGAGRTFTK EVVQAMATFN
     EKPIIFALSN PTSQSECTAE EAYSWSEGRA IFASGSPFAP VEYNGKVYAS GQANNAYIFP
     GFGLGLIISG AIRVHDDMLL VASEALADEV SQENFEKGTH IPPFSNIRKI SAHIAKVAAK
     AYELGLATRL PQPKDLVAYA ESCMYSPAYR SYR
//