Reviewed, UniProtKB/Swiss-Prot P37222 (MAOC_SOLLC)

Names and origin

Protein names	Recommended name:     NADP-dependent malic enzyme, chloroplastic       Short name=NADP-ME     EC=1.1.1.40
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	573 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO(2) released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO(2) that occurs in most plants.
Catalytic activity	(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Pathway	Photosynthesis; C4 acid pathway.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Ligand	Metal-binding NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 573	›573	NADP-dependent malic enzyme, chloroplastic		PRO_0000160199
Regions
	Nucleotide binding	318 – 334	17	NADP By similarity
Sites
	Active site	123	1	Proton donor By similarity
	Active site	194	1	Proton acceptor By similarity
	Metal binding	265	1	Divalent metal cation By similarity
	Metal binding	266	1	Divalent metal cation By similarity
	Metal binding	289	1	Divalent metal cation By similarity
	Binding site	176	1	NAD By similarity
	Binding site	289	1	NAD By similarity
	Binding site	430	1	NAD By similarity
	Site	289	1	Important for activity By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P37222-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 13846E61206F8E75	FASTA	573	63,561
	10 20 30 40 50 60 IRHESTVTGG VQDVYGEDSA TEDQSITPWT LSVASGFSLL RNPHYNKGLA FSERERDTHY 70 80 90 100 110 120 LRGLLPPVVI SHDLQVKKMM NSIRKYDVPL QRYMAMMDLQ EMNERLFYKL LIDNVEELLP 130 140 150 160 170 180 IVYTPTVGEA CQKYGWIFKR PQGLFFSLKE KGKIHEVLKN WPEKKIQVIV VTDGERILGL 190 200 210 220 230 240 GDLGCQGMGI PVGKLSLYSA LGGIRPSACL PVTIDVGQTM KFVDDEFYIG LRQRRATGQE 250 260 270 280 290 300 YSELLDEFMY AVKQNYGEKV LIQFEDFANH NAFNLLAKYG TSHLVFNDDI QGTASVVLAG 310 320 330 340 350 360 LMAALNLVGG SLSEHTFLFL GAGEAGTGIA ELIALEMSKQ TGIPLEETRK KIWMVDSKGL 370 380 390 400 410 420 IVKSRMEMLQ HFKRPWAHDH EPVQELVNAV KSIKPTVLIG SSGAGRTFTK EVVQAMATFN 430 440 450 460 470 480 EKPIIFALSN PTSQSECTAE EAYSWSEGRA IFASGSPFAP VEYNGKVYAS GQANNAYIFP 490 500 510 520 530 540 GFGLGLIISG AIRVHDDMLL VASEALADEV SQENFEKGTH IPPFSNIRKI SAHIAKVAAK 550 560 570 AYELGLATRL PQPKDLVAYA ESCMYSPAYR SYR

References

[1]	"Isolation of a cDNA clone for tomato fruit NADP+ malic enzyme." Finger F.L., Knee M., Lagrimini L.M.A. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Rutgers.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27509 mRNA. Translation: AAA34174.1.
PIR	T07088.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOC_SOLLC

Accession

Primary (citable) accession number: P37222

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)