Reviewed, UniProtKB/Swiss-Prot P37221 (MAOM_SOLTU)

Names and origin

Protein names	Recommended name:     NAD-dependent malic enzyme 62 kDa isoform, mitochondrial       Short name=NAD-ME     EC=1.1.1.39
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Heterodimer of two related subunits.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion
	Chain	35 – 626	592	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial		PRO_0000018543
Sites
	Active site	146	1	Proton donor By similarity
	Active site	217	1	Proton acceptor By similarity
	Metal binding	288	1	Divalent metal cation By similarity
	Metal binding	289	1	Divalent metal cation By similarity
	Metal binding	312	1	Divalent metal cation By similarity
	Binding site	199	1	NAD By similarity
	Binding site	312	1	NAD By similarity
	Binding site	467	1	NAD By similarity
	Site	312	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37221-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 8548B532B70A917C	FASTA	626	69,953
	10 20 30 40 50 60 MAIFSNQMRL SSTLLKRLHQ RVAAAVNSSS SRNFTTTEGH RPTIVHKRSL DILHDPWFNK 70 80 90 100 110 120 GTAFSFTERD RLHIRGLLPP NVMSFEQQIA RFMADLKRLE VQARDGPSDP YVLAKWRILN 130 140 150 160 170 180 RLHDRNETLY YKVLMENIEE YAPIVYTPTV GLVCQKYSGL FRRPRGMYFS AEDRGEMMSM 190 200 210 220 230 240 VYNWPADQVD MIVVTDGSRI LGLGDLGIQG IGIAIGKLDL YVAAAGINPQ RVLPVMIDVG 250 260 270 280 290 300 TDNENLLKDP LYLGLQDHRL DGEEYIEVID EFMEAVFTRW PHVIVQFEDF QSKWAFKLLQ 310 320 330 340 350 360 RYRNNYRMFN DDIQGTAGVA IAGLLGAVRA QGRPMIDFPK MKIVVAGAGS AGIGVLNAAR 370 380 390 400 410 420 KTMARMLGNT EIAFESARSQ FWVVDAKGLI TEARENVDPD ARPFARKIKE IERQGLSEGA 430 440 450 460 470 480 TLAEVVREVK PDVLLGLSAC GGLFSKEVLE ALKHSTSTRP AIFPMSNPTR NAECTPEEAF 490 500 510 520 530 540 SILGENIIFA SGSPFKDVDL GNGHVGHCNQ ANNMFLFPGI GLGTLLSGSR IVSDGMLQAA 550 560 570 580 590 600 AECLAAYITE EEVLKGIIYP SISRIRDITK EVAAAVVKEA IEEDLAEGYR EMDSRELRKL 610 620 DEAQISEFVE NNMWSPDYPT LVYKKD

References

[1]

"Plant mitochondrial NAD+-dependent malic enzyme. cDNA cloning, deduced primary structure of the 59- and 62-kDa subunits, import, gene complexity and expression analysis." Winning B.M., Bourguignon J., Leaver C.J. J. Biol. Chem. 269:4780-4786(1994) [PubMed: 8106447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Desiree. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z23023 mRNA. Translation: CAA80559.1.
PIR	B53318.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.39. 296.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOM_SOLTU

Accession

Primary (citable) accession number: P37221

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)