Reviewed, UniProtKB/Swiss-Prot P37215 (AROF_SOLLC)

Names and origin

Protein names	Recommended name:     Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic     EC=2.5.1.54 Alternative name(s):     Phospho-2-keto-3-deoxyheptonate aldolase 1     3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 1     DAHP synthase 1
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in the synthesis of secondary metabolites derived from intermediates of the pre-chorismate pathway up to shikimate.
Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Higher levels seen in the cotyledons than in the leaves and flowers. Lower levels seen in the roots and stems.
Sequence similarities	Belongs to the class-II DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 511	462	Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic		PRO_0000002300

Sequences

	Sequence		Length	Mass (Da)
	P37215-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: E7725992C42BEA4C	FASTA	511	57,120
	10 20 30 40 50 60 MALSNTLSLS SSKSLVQSHL LHNPTPQPRF SLFPTTQHGR RHPISAVHAA EPSKTAVKQG 70 80 90 100 110 120 KWSLDSWKTK KALQLPEYPD EKELESVLKT LEMNPPLVFA GEARSLEEKL GEAALGKAFL 130 140 150 160 170 180 LQGGDCAESF KEFNANNIRD TFRILLQMSV VLMFGGQVPV IKVGRMAGQF AKPRSDPFEE 190 200 210 220 230 240 INGVKLPSYK GDNINGDTFD EKSRIPDPHR LIRAYMQSAA TLNLLRAFAT GGYAAMQRVT 250 260 270 280 290 300 EWNLDFVENS EQGDRYQELA HRVDEALGFM AAAGLTVDHP IMSTTDFWTS HECLLLPYEQ 310 320 330 340 350 360 ALTREDSTSG LFYDCSAHMV WVGERTRQLD GAHVEFLRGV ANPLGIKVSQ KMDPKELIKL 370 380 390 400 410 420 IDILNPANKP GRITVIVRMG AENMRVKLSH LVRAVRGAGQ IVTWVCDPMH GNTIKAPCGL 430 440 450 460 470 480 KTRAFDSIQA EVRAFFDVHE QEGSHPWCIH LEMTGQNVTE CIGGSRTVTY DDLGSRYHTH 490 500 510 CDPRLNASQS LELSFIVAER LRRRRMSSQR L

References

[1]

"Differential expression of tomato (Lycopersicon esculentum L.) genes encoding shikimate pathway isoenzymes. I. 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase." Goerlach J., Beck A., Henstrand J.M., Handa A.K., Herrmann K.M., Schmid J., Amrhein N. Plant Mol. Biol. 23:697-706(1993) [PubMed: 7902741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. UC82B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21792 mRNA. Translation: CAA79855.1.
PIR	S40411.
UniGene	Les.4497
3D structure databases
SMR	P37215. Positions 42-502.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.54. 281054.
Family and domain databases
InterPro	IPR002480. DAHP_synth_2. [Graphical view]
PANTHER	PTHR21337. DAHP_synth_2. 1 hit.
Pfam	PF01474. DAHP_synth_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01358. DAHP_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROF_SOLLC

Accession

Primary (citable) accession number: P37215

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)