P37201 (NCPR_CANTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH--cytochrome P450 reductase
Short name=CPR
Short name=P450R
EC=1.6.2.4

Gene names

Name:	NCP1
Synonyms:	CPR

Organism

Candida tropicalis (Yeast)

Taxonomic identifier

5482 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	680 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Catalytic activity	NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
Cofactor	FAD. FMN.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein.
Sequence similarities	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 680	680	NADPH--cytochrome P450 reductase		PRO_0000167606
Regions
	Transmembrane	8 – 24	17	Helical; Potential
	Domain	60 – 204	145	Flavodoxin-like
	Domain	264 – 509	246	FAD-binding FR-type
	Nucleotide binding	149 – 180	32	FMN By similarity
	Nucleotide binding	304 – 315	12	FAD By similarity
	Nucleotide binding	435 – 446	12	FAD By similarity
	Nucleotide binding	532 – 550	19	NADP By similarity
	Nucleotide binding	628 – 644	17	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37201 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: AB31ABFF8ADA41DA	FASTA	680	76,689
	10 20 30 40 50 60 MALDKLDLYV IITLVVAIAA YFAKNQFLDQ QQDTGFLNTD SGDGNSRDIL QALKKNNKNT 70 80 90 100 110 120 LLLFGSQTGT AEDYANKLSR ELHSRFGLKT MVADFADYDF ENFGDITEDI LVFFIVATYG 130 140 150 160 170 180 EGEPTDNADE FHTWLTEEAD TLSTLKYTVF GLGNSTYEFF NAIGRKFDRL LGEKGGDRFA 190 200 210 220 230 240 EYGEGDDGTG TLDEDFLAWK DNVFDSLKND LNFEEKELKY EPNVKLTERD DLSGNDPDVS 250 260 270 280 290 300 LGEPNVKYIK SEGVDLTKGP FDHTHPFLAR IVKTKELFTS EDRHCVHVEF DISESNLKYT 310 320 330 340 350 360 TGDHLAIWPS NSDENIKQFA KCFGLEDKLD TVIELKALDS TYSIPFPNPI TYGAVIRHHL 370 380 390 400 410 420 EISGPVSRQF FLSIAGFAPD EETKKSFTRI GGDKQEFASK VTRRKFNIAD ALLFASNNRP 430 440 450 460 470 480 WSDVPFEFLI ENVQHLTPRY YSISSSSLSE KQTINVTAVV EAEEEADGRP VTGVVTNLLK 490 500 510 520 530 540 NIEIEQNKTG ETPMVHYDLN GPRGKFSKFR LPVHVRRSNF KLPKNSTTPV ILIGPGTGVA 550 560 570 580 590 600 PLRGFVRERV QQVKNGVNVG KTVLFYGCRN SEQDFLYKQE WSEYASVLGE NFEMFNAFSR 610 620 630 640 650 660 QDPTKKVYVQ DKILENSALV DELLSSGAII YVCGDASRMA RDVQAAIAKI VAKSRDIHED 670 680 KAAELVKSWK VQNRYQEDVW

References

[1]

"Isolation and characterization of the alkane-inducible NADPH-cytochrome P-450 oxidoreductase gene from Candida tropicalis. Identification of invariant residues within similar amino acid sequences of divergent flavoproteins." Sutter T.R., Sanglard D., Loper J.C. J. Biol. Chem. 265:16428-16436(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 750 / CBS 94 / DSM 11953 / JCM 1541 / NBRC 1400.

[2]

Erratum Sutter T.R., Sanglard D., Loper J.C. J. Biol. Chem. 265:22056-22056(1990)

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35199 Genomic DNA. Translation: AAA34333.1.
PIR	A37890.
3D structure databases
ProteinModelPortal	P37201.
SMR	P37201. Positions 46-680.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P37201.
Family and domain databases
Gene3D	1.20.990.10. 1 hit.
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR023173. NADPH_Cyt_P450_Rdtase_dom3. IPR001433. OxRdtase_FAD/NAD-bd. IPR023208. P450R. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF000208. P450R. 1 hit.
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCPR_CANTR

Accession

Primary (citable) accession number: P37201

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program