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P37201 (NCPR_CANTR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADPH--cytochrome P450 reductase

Short name=CPR
Short name=P450R
EC=1.6.2.4
Gene names
Name:NCP1
Synonyms:CPR
OrganismCandida tropicalis (Yeast)
Taxonomic identifier5482 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length680 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activity

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactor

FAD.

FMN.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein.

Sequence similarities

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 680680NADPH--cytochrome P450 reductase
PRO_0000167606

Regions

Transmembrane8 – 2417Helical; Potential
Domain60 – 204145Flavodoxin-like
Domain264 – 509246FAD-binding FR-type
Nucleotide binding149 – 18032FMN By similarity
Nucleotide binding304 – 31512FAD By similarity
Nucleotide binding435 – 44612FAD By similarity
Nucleotide binding532 – 55019NADP By similarity
Nucleotide binding628 – 64417NADP By similarity

Sequences

Sequence LengthMass (Da)Tools
P37201 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: AB31ABFF8ADA41DA

FASTA68076,689
        10         20         30         40         50         60 
MALDKLDLYV IITLVVAIAA YFAKNQFLDQ QQDTGFLNTD SGDGNSRDIL QALKKNNKNT 

        70         80         90        100        110        120 
LLLFGSQTGT AEDYANKLSR ELHSRFGLKT MVADFADYDF ENFGDITEDI LVFFIVATYG 

       130        140        150        160        170        180 
EGEPTDNADE FHTWLTEEAD TLSTLKYTVF GLGNSTYEFF NAIGRKFDRL LGEKGGDRFA 

       190        200        210        220        230        240 
EYGEGDDGTG TLDEDFLAWK DNVFDSLKND LNFEEKELKY EPNVKLTERD DLSGNDPDVS 

       250        260        270        280        290        300 
LGEPNVKYIK SEGVDLTKGP FDHTHPFLAR IVKTKELFTS EDRHCVHVEF DISESNLKYT 

       310        320        330        340        350        360 
TGDHLAIWPS NSDENIKQFA KCFGLEDKLD TVIELKALDS TYSIPFPNPI TYGAVIRHHL 

       370        380        390        400        410        420 
EISGPVSRQF FLSIAGFAPD EETKKSFTRI GGDKQEFASK VTRRKFNIAD ALLFASNNRP 

       430        440        450        460        470        480 
WSDVPFEFLI ENVQHLTPRY YSISSSSLSE KQTINVTAVV EAEEEADGRP VTGVVTNLLK 

       490        500        510        520        530        540 
NIEIEQNKTG ETPMVHYDLN GPRGKFSKFR LPVHVRRSNF KLPKNSTTPV ILIGPGTGVA 

       550        560        570        580        590        600 
PLRGFVRERV QQVKNGVNVG KTVLFYGCRN SEQDFLYKQE WSEYASVLGE NFEMFNAFSR 

       610        620        630        640        650        660 
QDPTKKVYVQ DKILENSALV DELLSSGAII YVCGDASRMA RDVQAAIAKI VAKSRDIHED 

       670        680 
KAAELVKSWK VQNRYQEDVW 

« Hide

References

[1]"Isolation and characterization of the alkane-inducible NADPH-cytochrome P-450 oxidoreductase gene from Candida tropicalis. Identification of invariant residues within similar amino acid sequences of divergent flavoproteins."
Sutter T.R., Sanglard D., Loper J.C.
J. Biol. Chem. 265:16428-16436(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 750 / CBS 94 / DSM 11953 / JCM 1541 / NBRC 1400.
[2]Erratum
Sutter T.R., Sanglard D., Loper J.C.
J. Biol. Chem. 265:22056-22056(1990)

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35199 Genomic DNA. Translation: AAA34333.1.
PIRA37890.

3D structure databases

ProteinModelPortalP37201.
SMRP37201. Positions 46-680.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP37201.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF000208. P450R. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNCPR_CANTR
AccessionPrimary (citable) accession number: P37201
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: February 19, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families