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Protein

NADPH--cytochrome P450 reductase

Gene

NCP1

Organism
Candida tropicalis (Yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

Protein has several cofactor binding sites:

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi149 – 18032FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi304 – 31512FADBy similarityAdd
BLAST
Nucleotide bindingi435 – 44612FADBy similarityAdd
BLAST
Nucleotide bindingi532 – 55019NADPBy similarityAdd
BLAST
Nucleotide bindingi628 – 64417NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

SABIO-RKP37201.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Gene namesi
Name:NCP1
Synonyms:CPR
OrganismiCandida tropicalis (Yeast)
Taxonomic identifieri5482 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2417HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 680680NADPH--cytochrome P450 reductasePRO_0000167606Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP37201.
SMRiP37201. Positions 46-680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 204145Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini264 – 509246FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37201-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALDKLDLYV IITLVVAIAA YFAKNQFLDQ QQDTGFLNTD SGDGNSRDIL
60 70 80 90 100
QALKKNNKNT LLLFGSQTGT AEDYANKLSR ELHSRFGLKT MVADFADYDF
110 120 130 140 150
ENFGDITEDI LVFFIVATYG EGEPTDNADE FHTWLTEEAD TLSTLKYTVF
160 170 180 190 200
GLGNSTYEFF NAIGRKFDRL LGEKGGDRFA EYGEGDDGTG TLDEDFLAWK
210 220 230 240 250
DNVFDSLKND LNFEEKELKY EPNVKLTERD DLSGNDPDVS LGEPNVKYIK
260 270 280 290 300
SEGVDLTKGP FDHTHPFLAR IVKTKELFTS EDRHCVHVEF DISESNLKYT
310 320 330 340 350
TGDHLAIWPS NSDENIKQFA KCFGLEDKLD TVIELKALDS TYSIPFPNPI
360 370 380 390 400
TYGAVIRHHL EISGPVSRQF FLSIAGFAPD EETKKSFTRI GGDKQEFASK
410 420 430 440 450
VTRRKFNIAD ALLFASNNRP WSDVPFEFLI ENVQHLTPRY YSISSSSLSE
460 470 480 490 500
KQTINVTAVV EAEEEADGRP VTGVVTNLLK NIEIEQNKTG ETPMVHYDLN
510 520 530 540 550
GPRGKFSKFR LPVHVRRSNF KLPKNSTTPV ILIGPGTGVA PLRGFVRERV
560 570 580 590 600
QQVKNGVNVG KTVLFYGCRN SEQDFLYKQE WSEYASVLGE NFEMFNAFSR
610 620 630 640 650
QDPTKKVYVQ DKILENSALV DELLSSGAII YVCGDASRMA RDVQAAIAKI
660 670 680
VAKSRDIHED KAAELVKSWK VQNRYQEDVW
Length:680
Mass (Da):76,689
Last modified:October 1, 1994 - v1
Checksum:iAB31ABFF8ADA41DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35199 Genomic DNA. Translation: AAA34333.1.
PIRiA37890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35199 Genomic DNA. Translation: AAA34333.1.
PIRiA37890.

3D structure databases

ProteinModelPortaliP37201.
SMRiP37201. Positions 46-680.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP37201.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of the alkane-inducible NADPH-cytochrome P-450 oxidoreductase gene from Candida tropicalis. Identification of invariant residues within similar amino acid sequences of divergent flavoproteins."
    Sutter T.R., Sanglard D., Loper J.C.
    J. Biol. Chem. 265:16428-16436(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 750 / CBS 94 / DSM 11953 / JCM 1541 / NBRC 1400.
  2. Erratum
    Sutter T.R., Sanglard D., Loper J.C.
    J. Biol. Chem. 265:22056-22056(1990)

Entry informationi

Entry nameiNCPR_CANTR
AccessioniPrimary (citable) accession number: P37201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.