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Protein

NADPH--cytochrome P450 reductase

Gene

NCP1

Organism
Candida tropicalis (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187FMNUniRule annotation1
Binding sitei283NADPUniRule annotation1
Binding sitei537NADPUniRule annotation1
Binding sitei642NADPUniRule annotation1
Binding sitei680FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 71FMNUniRule annotation6
Nucleotide bindingi117 – 120FMNUniRule annotation4
Nucleotide bindingi152 – 161FMNUniRule annotation10
Nucleotide bindingi439 – 442FADUniRule annotation4
Nucleotide bindingi457 – 459FADUniRule annotation3
Nucleotide bindingi473 – 476FADUniRule annotation4
Nucleotide bindingi599 – 600NADPUniRule annotation2
Nucleotide bindingi606 – 610NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

SABIO-RKP37201.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:NCP1UniRule annotation
Synonyms:CPR
OrganismiCandida tropicalis (Yeast)
Taxonomic identifieri5482 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Mitochondrion outer membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cell membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5LumenalUniRule annotation5
Transmembranei6 – 23HelicalUniRule annotationAdd BLAST18
Topological domaini24 – 680CytoplasmicUniRule annotationAdd BLAST657

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001676061 – 680NADPH--cytochrome P450 reductaseAdd BLAST680

Interactioni

Protein-protein interaction databases

STRINGi294747.XP_002545704.1.

Structurei

3D structure databases

ProteinModelPortaliP37201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 204Flavodoxin-likeUniRule annotationAdd BLAST145
Domaini264 – 509FAD-binding FR-typeUniRule annotationAdd BLAST246

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALDKLDLYV IITLVVAIAA YFAKNQFLDQ QQDTGFLNTD SGDGNSRDIL
60 70 80 90 100
QALKKNNKNT LLLFGSQTGT AEDYANKLSR ELHSRFGLKT MVADFADYDF
110 120 130 140 150
ENFGDITEDI LVFFIVATYG EGEPTDNADE FHTWLTEEAD TLSTLKYTVF
160 170 180 190 200
GLGNSTYEFF NAIGRKFDRL LGEKGGDRFA EYGEGDDGTG TLDEDFLAWK
210 220 230 240 250
DNVFDSLKND LNFEEKELKY EPNVKLTERD DLSGNDPDVS LGEPNVKYIK
260 270 280 290 300
SEGVDLTKGP FDHTHPFLAR IVKTKELFTS EDRHCVHVEF DISESNLKYT
310 320 330 340 350
TGDHLAIWPS NSDENIKQFA KCFGLEDKLD TVIELKALDS TYSIPFPNPI
360 370 380 390 400
TYGAVIRHHL EISGPVSRQF FLSIAGFAPD EETKKSFTRI GGDKQEFASK
410 420 430 440 450
VTRRKFNIAD ALLFASNNRP WSDVPFEFLI ENVQHLTPRY YSISSSSLSE
460 470 480 490 500
KQTINVTAVV EAEEEADGRP VTGVVTNLLK NIEIEQNKTG ETPMVHYDLN
510 520 530 540 550
GPRGKFSKFR LPVHVRRSNF KLPKNSTTPV ILIGPGTGVA PLRGFVRERV
560 570 580 590 600
QQVKNGVNVG KTVLFYGCRN SEQDFLYKQE WSEYASVLGE NFEMFNAFSR
610 620 630 640 650
QDPTKKVYVQ DKILENSALV DELLSSGAII YVCGDASRMA RDVQAAIAKI
660 670 680
VAKSRDIHED KAAELVKSWK VQNRYQEDVW
Length:680
Mass (Da):76,689
Last modified:October 1, 1994 - v1
Checksum:iAB31ABFF8ADA41DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35199 Genomic DNA. Translation: AAA34333.1.
PIRiA37890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35199 Genomic DNA. Translation: AAA34333.1.
PIRiA37890.

3D structure databases

ProteinModelPortaliP37201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi294747.XP_002545704.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.

Enzyme and pathway databases

SABIO-RKP37201.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCPR_CANTR
AccessioniPrimary (citable) accession number: P37201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 5, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.