Reviewed, UniProtKB/Swiss-Prot P37197 (YHJA_ECOLI)

Names and origin

Protein names

Recommended name:
    Probable cytochrome c peroxidase
    EC=1.11.1.5

Gene names

Name:	yhjA
Ordered Locus Names:	b3518, JW3486

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Catalytic activity	2 ferrocytochrome c + H2O2 = 2 ferricytochrome c + 2 H2O.
Post-translational modification	Binds 3 heme groups per subunit Potential.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW response to hydrogen peroxide Inferred from mutant phenotype. Source: UniProtKB transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-c peroxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Probable cytochrome c peroxidase		PRO_0000108444
Sites
	Metal binding	63	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	195	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	211	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	355	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	415	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	429	1	Iron (heme 3 axial ligand) By similarity
	Binding site	59	1	Heme 1 (covalent) By similarity
	Binding site	62	1	Heme 1 (covalent) By similarity
	Binding site	207	1	Heme 2 (covalent) By similarity
	Binding site	210	1	Heme 2 (covalent) By similarity
	Binding site	351	1	Heme 3 (covalent) By similarity
	Binding site	354	1	Heme 3 (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37197-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 9F494A698949E6DA	FASTA	465	51,571
	10 20 30 40 50 60 MKMVSRITAI GLAGVAICYL GLSGYVWYHD NKRSKQADVQ ASAVSENNKV LGFLREKGCD 70 80 90 100 110 120 YCHTPSAELP AYYYIPGAKQ LMDYDIKLGY KSFNLEAVRA ALLADKPVSQ SDLNKIEWVM 130 140 150 160 170 180 QYETMPPTRY TALHWAGKVS DEERAEILAW IAKQRAEYYA SNDTAPEHRN EPVQPIPQKL 190 200 210 220 230 240 PTDAQKVALG FALYHDPRLS ADSTISCAHC HALNAGGVDG RKTSIGVGGA VGPINAPTVF 250 260 270 280 290 300 NSVFNVEQFW DGRAATLQDQ AGGPPLNPIE MASKSWDEII AKLEKDPQLK TQFLEVYPQG 310 320 330 340 350 360 FSGENITDAI AEFEKTLITP DSPFDKWLRG DENALTAQQK KGYQLFKDNK CATCHGGIIL 370 380 390 400 410 420 GGRSFEPLGL KKDFNFGEIT AADIGRMNVT KEERDKLRQK VPGLRNVALT APYFHRGDVP 430 440 450 460 TLDGAVKLML RYQVGKELPQ EDVDDIVAFL HSLNGVYTPY MQDKQ

References

[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00039 Genomic DNA. Translation: AAB18494.1. U00096 Genomic DNA. Translation: AAC76543.1. AP009048 Genomic DNA. Translation: BAE77776.1.
PIR	S47738.
RefSeq	AP_004275.1. NP_417975.1.
3D structure databases
SMR	P37197. Positions 170-458.
ModBase	Search...
Protein-protein interaction databases
STRING	P37197.
Genome annotation databases
GeneID	948038.
GenomeReviews	Gene locus JW3486 in contig AP009048_GR. Gene locus b3518 in contig U00096_GR.
KEGG	ecj:JW3486. eco:b3518.
Organism-specific databases
EchoBASE	EB2155.
EcoGene	EG12244. yhjA.
CMR	Search...
Phylogenomic databases
eggNOG	COG1858.
HOGENOM	HBG624620.
OMA	NPIEMGS.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12244-MONOMER. ECOL168927:B3518-MONOMER.
Gene expression databases
Genevestigator	P37197.
Family and domain databases
InterPro	IPR009056. Cyt_c_monohaem. IPR004852. Di-haem_cyt_c_peroxidsae. [Graphical view]
Gene3D	G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.
Pfam	PF03150. CCP_MauG. 1 hit. [Graphical view]
PROSITE	PS51007. CYTC. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YHJA_ECOLI

Accession

Primary (citable) accession number: P37197
Secondary accession number(s): Q2M7I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)