Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PTS system galactitol-specific EIIB component

Gene

gatB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. The enzyme II complex composed of GatA, GatB and GatC is involved in galactitol transport. It can also use D-glucitol.2 Publications

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + galactitol(Side 1) = [protein]-L-histidine + galactitol 1-phosphate(Side 2).1 Publication

Kineticsi

  1. KM=4.4 µM for D-galactitol1 Publication
  2. KM=800 µM for D-glucitol1 Publication
  1. Vmax=3.2 nmol/min/mg enzyme with D-galactitol as substrate1 Publication
  2. Vmax=3.2 nmol/min/mg enzyme with D-glucitol as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91Phosphocysteine intermediate; for EIIB activityCurated

GO - Molecular functioni

GO - Biological processi

  • galactitol metabolic process Source: UniProtKB-KW
  • galactitol transport Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Galactitol metabolism, Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GATB-MONOMER.
ECOL316407:JW2077-MONOMER.
MetaCyc:GATB-MONOMER.

Protein family/group databases

TCDBi4.A.5.1.1. the pts galactitol (gat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system galactitol-specific EIIB component1 Publication
Alternative name(s):
EIIB-Gat1 Publication
Galactitol-specific phosphotransferase enzyme IIB component1 Publication (EC:2.7.1.2001 Publication)
Gene namesi
Name:gatB1 Publication
Ordered Locus Names:b2093, JW2077
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12415. gatB.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9494PTS system galactitol-specific EIIB componentPRO_0000186572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91Phosphocysteine; by EIIACurated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP37188.

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Subunit structurei

Forms a complex with one each of subunit of GatA, GatB and 2 subunits of GatC.1 Publication

Protein-protein interaction databases

BioGridi4263510. 9 interactions.
DIPiDIP-9744N.
IntActiP37188. 7 interactions.
MINTiMINT-1222883.
STRINGi511145.b2093.

Structurei

3D structure databases

ProteinModelPortaliP37188.
SMRiP37188. Positions 2-94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9494PTS EIIB type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41090UW. Bacteria.
COG3414. LUCA.
HOGENOMiHOG000230475.
KOiK02774.
OMAiICTTAKI.
PhylomeDBiP37188.

Family and domain databases

InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37188-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKIIVACG GAVATSTMAA EEIKELCQNH NIPVELIQCR VNEIETYMDG
60 70 80 90
VHLICTTAKV DRSFGDIPLV HGMPFISGIG IEALQNKILT ILQG
Length:94
Mass (Da):10,222
Last modified:November 1, 1997 - v2
Checksum:iA8156F04894F0CD7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91C → E AA sequence (PubMed:9868784).Curated
Sequence conflicti29 – 291N → S in CAA56229 (PubMed:7772602).Curated
Sequence conflicti59 – 591K → R in CAA56229 (PubMed:7772602).Curated
Sequence conflicti76 – 761I → V in CAA56229 (PubMed:7772602).Curated
Sequence conflicti79 – 791I → V in CAA56229 (PubMed:7772602).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA. Translation: CAA56229.1.
U00096 Genomic DNA. Translation: AAC75154.1.
AP009048 Genomic DNA. Translation: BAA15956.1.
PIRiD64976.
S55904.
RefSeqiNP_416596.1. NC_000913.3.
WP_000823270.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75154; AAC75154; b2093.
BAA15956; BAA15956; BAA15956.
GeneIDi946610.
KEGGiecj:JW2077.
eco:b2093.
PATRICi32119519. VBIEscCol129921_2170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA. Translation: CAA56229.1.
U00096 Genomic DNA. Translation: AAC75154.1.
AP009048 Genomic DNA. Translation: BAA15956.1.
PIRiD64976.
S55904.
RefSeqiNP_416596.1. NC_000913.3.
WP_000823270.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37188.
SMRiP37188. Positions 2-94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263510. 9 interactions.
DIPiDIP-9744N.
IntActiP37188. 7 interactions.
MINTiMINT-1222883.
STRINGi511145.b2093.

Protein family/group databases

TCDBi4.A.5.1.1. the pts galactitol (gat) family.

Proteomic databases

PaxDbiP37188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75154; AAC75154; b2093.
BAA15956; BAA15956; BAA15956.
GeneIDi946610.
KEGGiecj:JW2077.
eco:b2093.
PATRICi32119519. VBIEscCol129921_2170.

Organism-specific databases

EchoBASEiEB2314.
EcoGeneiEG12415. gatB.

Phylogenomic databases

eggNOGiENOG41090UW. Bacteria.
COG3414. LUCA.
HOGENOMiHOG000230475.
KOiK02774.
OMAiICTTAKI.
PhylomeDBiP37188.

Enzyme and pathway databases

BioCyciEcoCyc:GATB-MONOMER.
ECOL316407:JW2077-MONOMER.
MetaCyc:GATB-MONOMER.

Miscellaneous databases

PROiP37188.

Family and domain databases

InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTKB_ECOLI
AccessioniPrimary (citable) accession number: P37188
Secondary accession number(s): P76412
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.