Galactitol-specific phosphotransferase enzyme IIB component

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Reviewed, UniProtKB/Swiss-Prot P37188 (PTKB_ECOLI)

Last modified November 3, 2009. Version 86. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Galactitol-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
Alternative name(s):
    EIIB-Gat
    PTS system galactitol-specific EIIB component

Gene names

Name:	gatB
Ordered Locus Names:	b2093, JW2077

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in galactitol transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain.

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Ontologies

Keywords
Biological process	Galactitol metabolism Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	galactitol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 94

Galactitol-specific phosphotransferase enzyme IIB component

PRO_0000186572

Regions

Domain

1 – 94

PTS EIIB type-2

Experimental info

Sequence conflict

C → E AA sequence Ref.5

Sequence conflict

N → S in CAA56229. Ref.1

Sequence conflict

K → R in CAA56229. Ref.1

Sequence conflict

I → V in CAA56229. Ref.1

Sequence conflict

I → V in CAA56229. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P37188-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: A8156F04894F0CD7
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FASTA

10,222

        10         20         30         40         50         60 
MKRKIIVACG GAVATSTMAA EEIKELCQNH NIPVELIQCR VNEIETYMDG VHLICTTAKV 

        70         80         90 
DRSFGDIPLV HGMPFISGIG IEALQNKILT ILQG

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the gat operon for galactitol utilization from a wild-type strain EC3132 of Escherichia coli." Nobelmann B., Lengeler J.W. Biochim. Biophys. Acta 1262:69-72(1995) [PubMed: 7772602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EC3132.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Small genes/gene-products in Escherichia coli K-12." Wasinger V.C., Humphery-Smith I. FEMS Microbiol. Lett. 169:375-382(1998) [PubMed: 9868784] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20. Strain: K12.

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Cross-references

Sequence databases
	X79837 Genomic DNA. Translation: CAA56229.1. U00096 Genomic DNA. Translation: AAC75154.1. AP009048 Genomic DNA. Translation: BAA15956.1.
PIR	D64976. S55904.
RefSeq	AP_002689.1. NP_416596.1.
3D structure databases
SMR	P37188. Positions 2-94.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9744N.
STRING	P37188.
Protein family/group databases
TCDB	4.A.5.1.1. PTS galactitol (Gat) family.
PTM databases
PhosSite	P37188.
Genome annotation databases
GeneID	946610.
GenomeReviews	Gene locus JW2077 in contig AP009048_GR. Gene locus b2093 in contig U00096_GR.
KEGG	ecj:JW2077. eco:b2093.
Organism-specific databases
EchoBASE	EB2314.
EcoGene	EG12415. gatB.
CMR	Search...
Phylogenomic databases
HOGENOM	P37188.
OMA	PFISGVG.
Enzyme and pathway databases
BioCyc	EcoCyc:GATB-MON.
Gene expression databases
Genevestigator	P37188.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003501. PTS_IIB_lac. [Graphical view]
Pfam	PF02302. PTS_IIB. 1 hit. [Graphical view]
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

PTKB_ECOLI

Accession

Primary (citable) accession number: P37188
Secondary accession number(s): P76412

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents