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Protein

Hydrogenase 2 maturation protease

Gene

hybD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease involved in the C-terminal processing of HybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of HybC.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16Nickel1
Metal bindingi62Nickel1
Metal bindingi93Nickel1

GO - Molecular functioni

GO - Biological processi

  • cellular protein modification process Source: GO_Central
  • protein processing Source: EcoCyc

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
LigandMetal-binding, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:EG11802-MONOMER
BRENDAi3.4.23.B20 2026

Protein family/group databases

MEROPSiA31.001

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase 2 maturation protease (EC:3.4.23.-)
Gene namesi
Name:hybD
Ordered Locus Names:b2993, JW2961
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11802 hybD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002019431 – 164Hydrogenase 2 maturation proteaseAdd BLAST164

Proteomic databases

PaxDbiP37182
PRIDEiP37182

Interactioni

Protein-protein interaction databases

BioGridi4262374, 54 interactors
DIPiDIP-9967N
IntActiP37182, 6 interactors
STRINGi316385.ECDH10B_3170

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi14 – 17Combined sources4
Helixi18 – 29Combined sources12
Beta strandi36 – 42Combined sources7
Helixi46 – 48Combined sources3
Helixi49 – 52Combined sources4
Beta strandi56 – 63Combined sources8
Beta strandi74 – 78Combined sources5
Helixi81 – 86Combined sources6
Helixi92 – 106Combined sources15
Beta strandi112 – 119Combined sources8
Beta strandi126 – 129Combined sources4
Helixi132 – 135Combined sources4
Helixi138 – 150Combined sources13
Turni151 – 153Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CFZX-ray2.20A/B/C/D/E/F1-159[»]
ProteinModelPortaliP37182
SMRiP37182
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37182

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A31 family.Curated

Phylogenomic databases

eggNOGiENOG4108USZ Bacteria
COG0680 LUCA
HOGENOMiHOG000278719
InParanoidiP37182
KOiK03605
OMAiRKMSSHQ
PhylomeDBiP37182

Family and domain databases

Gene3Di3.40.50.1450, 1 hit
InterProiView protein in InterPro
IPR004419 Pept_A31_hyd_express
IPR023430 Pept_HybD-like_dom_sf
IPR000671 Peptidase_A31
PANTHERiPTHR30302 PTHR30302, 1 hit
PfamiView protein in Pfam
PF01750 HycI, 1 hit
PRINTSiPR00446 HYDRGNUPTAKE
SUPFAMiSSF53163 SSF53163, 1 hit
TIGRFAMsiTIGR00140 hupD, 1 hit
TIGR00072 hydrog_prot, 1 hit

Sequencei

Sequence statusi: Complete.

P37182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILVLGVGN ILLTDEAIGV RIVEALEQRY ILPDYVEILD GGTAGMELLG
60 70 80 90 100
DMANRDHLII ADAIVSKKNA PGTMMILRDE EVPALFTNKI SPHQLGLADV
110 120 130 140 150
LSALRFTGEF PKKLTLVGVI PESLEPHIGL TPTVEAMIEP ALEQVLAALR
160
ESGVEAIPRE AIHD
Length:164
Mass (Da):17,751
Last modified:October 1, 1996 - v2
Checksum:iF390F66529380FA8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160 – 164EAIHD → SDS in AAA21592 (PubMed:8021226).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA Translation: AAA21592.1
U28377 Genomic DNA Translation: AAA69160.1
U00096 Genomic DNA Translation: AAC76029.1
AP009048 Genomic DNA Translation: BAE77054.1
PIRiG65085
RefSeqiNP_417467.1, NC_000913.3
WP_001221939.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76029; AAC76029; b2993
BAE77054; BAE77054; BAE77054
GeneIDi948982
KEGGiecj:JW2961
eco:b2993
PATRICifig|1411691.4.peg.3736

Entry informationi

Entry nameiHYBD_ECOLI
AccessioniPrimary (citable) accession number: P37182
Secondary accession number(s): Q2M9K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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