P37161 (LYSX_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme X
EC=3.2.1.17

Alternative name(s):
1,4-beta-N-acetylmuramidase X

Gene names

Name:	LysX
ORF Names:	CG9120

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Unlikely to play an active role in the humoral immune defense. May have a function in the digestion of bacteria in the food. May be involved in the clearance of bacteria from the larval gut before metamorphosis.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Tissue specificity	Found in the midgut.
Developmental stage	Rises dramatically in the late third instar, then decreases gradually during the pupal stages. Low expression is found in adults.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 142	123	Lysozyme X		PRO_0000018516
Sites
	Active site	51	1	By similarity
	Active site	69	1	By similarity
Amino acid modifications
	Disulfide bond	25 ↔ 140		By similarity
	Disulfide bond	46 ↔ 130		By similarity
	Disulfide bond	81 ↔ 97		By similarity
	Disulfide bond	93 ↔ 111		By similarity
Natural variations
	Natural variant	6	1	G → T in strain: G140. Ref.1
	Natural variant	78	1	M → L in strain: Canton-S, G02, G130 and G140. Ref.1
	Natural variant	94	1	D → G in strain: G130 and G140. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37161 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 2A48035364B995BC	FASTA	142	15,591
	10 20 30 40 50 60 MRALLGICVL ALVTPAVLGR TMDRCSLARE MANMGVSRDQ LSKWACIAEH ESSYRTGVVG 70 80 90 100 110 120 PPNTDGSNDY GIFQINDMYW CQPSSGKFSH NGCDVSCNAL LTDDIKSSVR CALKVLGQQG 130 140 WSAWSTWHYC SGYLPPIDDC FV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A screen for immunity genes evolving under positive selection in Drosophila." Jiggins F.M., Kim K.W. J. Evol. Biol. 20:965-970(2007) [PubMed: 17465907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-6; LEU-78 AND GLY-94. Strain: G02, G130, G140 and G28.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Larva and Pupae.
[5]	"The lysozyme locus in Drosophila melanogaster: an expanded gene family adapted for expression in the digestive tract." Daffre S., Kylsten P., Samakovlis C., Hultmark D. Mol. Gen. Genet. 242:152-162(1994) [PubMed: 8159165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-142. Strain: Canton-S.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM412869 Genomic DNA. Translation: CAL85492.1. AM412870 Genomic DNA. Translation: CAL85493.1. AM412871 Genomic DNA. Translation: CAL85494.1. AM412872 Genomic DNA. Translation: CAL85495.1. AE014296 Genomic DNA. Translation: AAF47445.1. AY119081 mRNA. Translation: AAM50941.1. Z22224 mRNA. Translation: CAA80226.1.
PIR	S41580.
RefSeq	NP_523881.1. NM_079157.2.
UniGene	Dm.19201.
3D structure databases
ProteinModelPortal	P37161.
SMR	P37161. Positions 20-141.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24095N.
MINT	MINT-1674138.
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0072637; FBpp0072533; FBgn0004431.
GeneID	38122.
KEGG	dme:Dmel_CG9120.
NMPDR	fig|7227.3.peg.7483.
Organism-specific databases
CTD	38122.
FlyBase	FBgn0004431. LysX.
Phylogenomic databases
eggNOG	inNOG11130.
GeneTree	EMGT00050000002113.
InParanoid	P37161.
OMA	TIADWIC.
OrthoDB	EOG476HGK.
PhylomeDB	P37161.
Gene expression databases
Bgee	P37161.
GermOnline	CG9120. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. IPR023346. Lysozyme-like_dom. [Graphical view]
KO	K01185.
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	807080.

Entry information

Entry name

LYSX_DROME

Accession

Primary (citable) accession number: P37161
Secondary accession number(s): A4V9W9 , A4V9X0, A4V9X1, A4V9X2, Q9W0K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project