Reviewed, UniProtKB/Swiss-Prot P37156 (LYSC1_TACAC)

Names and origin

Protein names	Recommended name:     Lysozyme C I     EC=3.2.1.17 Alternative name(s):     1,4-beta-N-acetylmuramidase C
Organism	Tachyglossus aculeatus aculeatus (Australian echidna)
Taxonomic identifier	49271 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Monotremata › Tachyglossidae › Tachyglossus

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase Milk protein
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 125

125

Lysozyme C I

PRO_0000208857

Sites

Active site

35

1

Active site

52

1

Amino acid modifications

Disulfide bond

9 ↔ 125

Disulfide bond

30 ↔ 115

Disulfide bond

64 ↔ 80

Disulfide bond

76 ↔ 94

Secondary structure

1

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125

Helix Strand Turn

Details...

Helix

5 – 14

10

Helix

25 – 36

12

Beta strand

43 – 45

3

Beta strand

51 – 53

3

Turn

54 – 57

4

Turn

60 – 63

4

Helix

80 – 83

4

Beta strand

84 – 86

3

Helix

89 – 102

14

Helix

109 – 114

6

Turn

115 – 117

3

Helix

121 – 123

3

Sequences

	Sequence		Length	Mass (Da)
	P37156-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 18D8A1DBA3724073	FASTA	125	13,994
	10 20 30 40 50 60 KILKKQELCK NLVAQGMNGY QHITLPNWVC TAFHESSYNT RATNHNTDGS TDYGILQINS 70 80 90 100 110 120 RYWCHDGKTP GSKNACNISC SKLLDDDITD DLKCAKKIAG EAKGLTPWVA WKSKCRGHDL SKFKC

References

[1]	"The isolation and amino acid sequences of echidna (Tachyglossus aculeatus) milk lysozyme I and II." Teahan C.G., McKenzie H.A., Shaw D.C., Griffiths M. Biochem. Int. 24:85-95(1991) [PubMed: 1768265] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Milk.
[2]	"Structure of the calcium-binding Echidna milk lysozyme at 1.9-A resolution." Guss J.M., Messer M., Costello M., Hardy K., Kumar V. Acta Crystallogr. D 53:355-363(1997) [PubMed: 15299900] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[3]	Erratum Guss J.M., Messer M., Costello M., Hardy K., Kumar V. Acta Crystallogr. D 53:805-805(1997)

Cross-references

Sequence databases

PIR

A45660.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JUG	X-ray	1.90	A	1-125	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH22. Glycoside Hydrolase Family 22.

Phylogenomic databases

HOVERGEN

P37156.

Enzyme and pathway databases

BRENDA

3.2.1.17. 306500.

Family and domain databases

InterPro

IPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000974. Glyco_hydro_22_lys.
[Graphical view]

Pfam

PF00062. Lys. 1 hit.
[Graphical view]

PRINTS

PR00137. LYSOZYME.
PR00135. LYZLACT.

SMART

SM00263. LYZ1. 1 hit.
[Graphical view]

PROSITE

PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LYSC1_TACAC

Accession

Primary (citable) accession number: P37156

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)