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Protein

N-acetylmuramoyl-L-alanine amidase CwlM

Gene

cwlM

Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase CwlM (EC:3.5.1.28)
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene namesi
Name:cwlM
OrganismiBacillus licheniformis
Taxonomic identifieri1402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

  • Secreted Curated

  • Note: Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001644201 – 253N-acetylmuramoyl-L-alanine amidase CwlMAdd BLAST253

Structurei

3D structure databases

ProteinModelPortaliP37134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 253SPORAdd BLAST75
Repeati184 – 2191Add BLAST36
Repeati220 – 2532Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 2532 X 35 AA approximate tandem repeatsAdd BLAST70

Sequence similaritiesi

Contains 1 SPOR domain.Curated

Keywords - Domaini

Repeat

Family and domain databases

CDDicd02696. MurNAc-LAA. 1 hit.
Gene3Di3.30.70.1070. 1 hit.
3.40.630.40. 1 hit.
InterProiIPR002508. MurNAc-LAA_cat.
IPR007730. SPOR_dom.
[Graphical view]
PfamiPF01520. Amidase_3. 1 hit.
PF05036. SPOR. 1 hit.
[Graphical view]
SMARTiSM00646. Ami_3. 1 hit.
[Graphical view]
SUPFAMiSSF110997. SSF110997. 1 hit.
PROSITEiPS51724. SPOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKIFIDPGH GGSDTGASAN GLQEKQLTLQ TALALRNMLL NEYQNVSVLL
60 70 80 90 100
SRTSDQTVSL TQRTNAANSW GADYFLSIHM NAGGGTGFED YIYPGVGAPT
110 120 130 140 150
TTYRDIMHEE ILKVVDFRDR GKKTANFHVL RETAMPALLT ENGFVDNTND
160 170 180 190 200
AEKLKSSAFI QSIARGHANG LARAFNLSKN AAALYKVQIA AFRTKANADS
210 220 230 240 250
LAAQAEAKGF DALVIYRDSL YKVQIGAFSS KENAEALVQQ AKNAEFDTFI

YQE
Length:253
Mass (Da):27,600
Last modified:October 1, 1994 - v1
Checksum:i28E86E017B68D768
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62116 Genomic DNA. Translation: CAA44026.1.
PIRiS23572.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62116 Genomic DNA. Translation: CAA44026.1.
PIRiS23572.

3D structure databases

ProteinModelPortaliP37134.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd02696. MurNAc-LAA. 1 hit.
Gene3Di3.30.70.1070. 1 hit.
3.40.630.40. 1 hit.
InterProiIPR002508. MurNAc-LAA_cat.
IPR007730. SPOR_dom.
[Graphical view]
PfamiPF01520. Amidase_3. 1 hit.
PF05036. SPOR. 1 hit.
[Graphical view]
SMARTiSM00646. Ami_3. 1 hit.
[Graphical view]
SUPFAMiSSF110997. SSF110997. 1 hit.
PROSITEiPS51724. SPOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCWLM_BACLI
AccessioniPrimary (citable) accession number: P37134
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 5, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.