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Protein

GDP-mannose pyrophosphatase NudK

Gene

nudK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GDP-mannose. Can also use other substrates, catalyzing the hydrolysis of the pyrophosphate bond, releasing a nucleoside monophosphate and a phosphorylated moiety, depending on the substrate.1 Publication

Catalytic activityi

GDP-mannose + H2O = GMP + mannose-1-phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=0.81 mM for GDP-mannose1 Publication
  1. Vmax=207 µmol/min/mg enzyme with GDP-mannose as substrate1 Publication

pH dependencei

Optimum pH is about 8.5.1 Publication

GO - Molecular functioni

  • GDP-mannose hydrolase activity Source: EcoCyc
  • magnesium ion binding Source: EcoCyc

Keywordsi

Molecular functionHydrolase
LigandMagnesium

Enzyme and pathway databases

BioCyciEcoCyc:EG12410-MONOMER
MetaCyc:EG12410-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-mannose pyrophosphatase NudK (EC:3.6.1.-)
Gene namesi
Name:nudK
Synonyms:yffH
Ordered Locus Names:b2467, JW2451
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12410 nudK

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001692311 – 191GDP-mannose pyrophosphatase NudKAdd BLAST191

Proteomic databases

PaxDbiP37128
PRIDEiP37128

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260924, 45 interactors
IntActiP37128, 10 interactors
STRINGi316385.ECDH10B_2632

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 14Combined sources10
Beta strandi16 – 28Combined sources13
Beta strandi30 – 32Combined sources3
Beta strandi34 – 43Combined sources10
Beta strandi47 – 54Combined sources8
Turni55 – 58Combined sources4
Beta strandi59 – 66Combined sources8
Helixi68 – 71Combined sources4
Turni72 – 74Combined sources3
Beta strandi79 – 82Combined sources4
Beta strandi84 – 87Combined sources4
Helixi93 – 104Combined sources12
Beta strandi112 – 120Combined sources9
Turni122 – 124Combined sources3
Beta strandi128 – 135Combined sources8
Helixi138 – 140Combined sources3
Beta strandi152 – 159Combined sources8
Helixi160 – 169Combined sources10
Helixi175 – 187Combined sources13
Turni188 – 190Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VIUX-ray2.40A/B/C/D2-191[»]
3O52X-ray2.50A/B/C/D/E1-191[»]
3O61X-ray2.45A/B/C/D1-191[»]
3O69X-ray2.10A/B1-191[»]
3O6ZX-ray2.05A/B1-191[»]
ProteinModelPortaliP37128
SMRiP37128
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37128

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 180Nudix hydrolasePROSITE-ProRule annotationAdd BLAST138

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi86 – 106Nudix boxAdd BLAST21

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudK subfamily.Curated

Phylogenomic databases

eggNOGiENOG4107VIJ Bacteria
ENOG410YMTX LUCA
HOGENOMiHOG000045855
InParanoidiP37128
KOiK12945
OMAiRTPAACI
PhylomeDBiP37128

Family and domain databases

InterProiView protein in InterPro
IPR004385 NDP_pyrophosphatase
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF00293 NUDIX, 1 hit
SUPFAMiSSF55811 SSF55811, 1 hit
TIGRFAMsiTIGR00052 TIGR00052, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit

Sequencei

Sequence statusi: Complete.

P37128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQQITLIKD KILSDNYFTL HNITYDLTRK DGEVIRHKRE VYDRGNGATI
60 70 80 90 100
LLYNTKKKTV VLIRQFRVAT WVNGNESGQL IESCAGLLDN DEPEVCIRKE
110 120 130 140 150
AIEETGYEVG EVRKLFELYM SPGGVTELIH FFIAEYSDNQ RANAGGGVED
160 170 180 190
EDIEVLELPF SQALEMIKTG EIRDGKTVLL LNYLQTSHLM D
Length:191
Mass (Da):21,749
Last modified:November 1, 1997 - v2
Checksum:i13880625B41233C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75520.1
AP009048 Genomic DNA Translation: BAA16341.1
L34011 Genomic DNA Translation: AAB46945.1
PIRiB65022
RefSeqiNP_416962.1, NC_000913.3
WP_001300814.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75520; AAC75520; b2467
BAA16341; BAA16341; BAA16341
GeneIDi947072
KEGGiecj:JW2451
eco:b2467
PATRICifig|1411691.4.peg.4273

Similar proteinsi

Entry informationi

Entry nameiNUDK_ECOLI
AccessioniPrimary (citable) accession number: P37128
Secondary accession number(s): P77255
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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