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Protein

Protein AegA

Gene

aegA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi15 – 151Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi18 – 181Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi22 – 221Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi56 – 561Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi59 – 591Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi64 – 641Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi68 – 681Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi87 – 871Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi90 – 901Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi93 – 931Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi97 – 971Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi121 – 1211Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi124 – 1241Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi133 – 1331Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi137 – 1371Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12409-MONOMER.
ECOL316407:JW2452-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AegA
Gene namesi
Name:aegA
Synonyms:yffG
Ordered Locus Names:b2468, JW2452
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12409. aegA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 659659Protein AegAPRO_0000170801Add
BLAST

Proteomic databases

PaxDbiP37127.
PRIDEiP37127.

Interactioni

Protein-protein interaction databases

BioGridi4260929. 9 interactions.
DIPiDIP-9060N.
IntActiP37127. 6 interactions.
STRINGi511145.b2468.

Structurei

3D structure databases

ProteinModelPortaliP37127.
SMRiP37127. Positions 1-139, 202-651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 32304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini47 – 77314Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini78 – 107304Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd
BLAST
Domaini114 – 147344Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZ8. Bacteria.
COG0493. LUCA.
COG1142. LUCA.
HOGENOMiHOG000031439.
InParanoidiP37127.
OMAiVESRYRY.
OrthoDBiEOG6XSZQF.
PhylomeDBiP37127.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR028261. DPD_II.
IPR023753. FAD/NAD-binding_dom.
IPR006006. Glut_synth_ssu2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
PF14691. Fer4_20. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01318. gltD_gamma_fam. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37127-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRFIMANSQ QCLGCHACEI ACVMAHNDEQ HVLSQHHFHP RITVIKHQQQ
60 70 80 90 100
RSAVTCHHCE DAPCARSCPN GAISHVDDSI QVNQQKCIGC KSCVVACPFG
110 120 130 140 150
TMQIVLTPVA AGKVKATAHK CDLCAGRENG PACVENCPAD ALQLVTDVAL
160 170 180 190 200
SGMAKSRRLR TARQEHQPWH ASTAAQEMPV MSKVEQMQAT PARGEPDKLA
210 220 230 240 250
IEARKTGFDE IYLPFRADQA QREASRCLKC GEHSVCEWTC PLHNHIPQWI
260 270 280 290 300
ELVKAGNIDA AVELSHQTNT LPEITGRVCP QDRLCEGACT IRDEHGAVTI
310 320 330 340 350
GNIERYISDQ ALAKGWRPDL SHVTKVDKRV AIIGAGPAGL ACADVLTRNG
360 370 380 390 400
VGVTVYDRHP EIGGLLTFGI PSFKLDKSLL ARRREIFSAM GIHFELNCEV
410 420 430 440 450
GKDVSLDSLL EQYDAVFVGV GTYRSMKAGL PNEDAPGVYD ALPFLIANTK
460 470 480 490 500
QVMGLEELPE EPFINTAGLN VVVLGGGDTA MDCVRTALRH GASNVTCAYR
510 520 530 540 550
RDEANMPGSK KEVKNAREEG ANFEFNVQPV ALELNEQGHV CGIRFLRTRL
560 570 580 590 600
GEPDAQGRRR PVPVEGSEFV MPADAVIMAF GFNPHGMPWL ESHGVTVDKW
610 620 630 640 650
GRIIADVESQ YRYQTTNPKI FAGGDAVRGA DLVVTAMAEG RHAAQGIIDW

LGVKSVKSH
Length:659
Mass (Da):71,844
Last modified:November 1, 1997 - v2
Checksum:iDD3ADF3E90218402
GO

Sequence cautioni

The sequence AAB46944.1 differs from that shown. Reason: Frameshift at position 602. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti488 – 4881L → R in AAB46944 (PubMed:8955321).Curated
Sequence conflicti513 – 5131V → A in AAB46944 (PubMed:8955321).Curated
Sequence conflicti600 – 6001W → M (PubMed:8955321).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34011 Genomic DNA. Translation: AAB46944.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75521.1.
AP009048 Genomic DNA. Translation: BAA16342.2.
PIRiC65022.
RefSeqiNP_416963.1. NC_000913.3.
WP_001078880.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75521; AAC75521; b2468.
BAA16342; BAA16342; BAA16342.
GeneIDi947383.
KEGGiecj:JW2452.
eco:b2468.
PATRICi32120319. VBIEscCol129921_2562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34011 Genomic DNA. Translation: AAB46944.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75521.1.
AP009048 Genomic DNA. Translation: BAA16342.2.
PIRiC65022.
RefSeqiNP_416963.1. NC_000913.3.
WP_001078880.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37127.
SMRiP37127. Positions 1-139, 202-651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260929. 9 interactions.
DIPiDIP-9060N.
IntActiP37127. 6 interactions.
STRINGi511145.b2468.

Proteomic databases

PaxDbiP37127.
PRIDEiP37127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75521; AAC75521; b2468.
BAA16342; BAA16342; BAA16342.
GeneIDi947383.
KEGGiecj:JW2452.
eco:b2468.
PATRICi32120319. VBIEscCol129921_2562.

Organism-specific databases

EchoBASEiEB2308.
EcoGeneiEG12409. aegA.

Phylogenomic databases

eggNOGiENOG4105BZ8. Bacteria.
COG0493. LUCA.
COG1142. LUCA.
HOGENOMiHOG000031439.
InParanoidiP37127.
OMAiVESRYRY.
OrthoDBiEOG6XSZQF.
PhylomeDBiP37127.

Enzyme and pathway databases

BioCyciEcoCyc:EG12409-MONOMER.
ECOL316407:JW2452-MONOMER.

Miscellaneous databases

PROiP37127.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR028261. DPD_II.
IPR023753. FAD/NAD-binding_dom.
IPR006006. Glut_synth_ssu2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
PF14691. Fer4_20. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01318. gltD_gamma_fam. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the aegA locus of Escherichia coli: control of gene expression in response to anaerobiosis and nitrate."
    Cavicchioli R., Kolesnikow T., Gunsalus R.P.
    J. Bacteriol. 178:6968-6974(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiAEGA_ECOLI
AccessioniPrimary (citable) accession number: P37127
Secondary accession number(s): P76560, P76970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: February 17, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.