Protein aegA - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P37127 (AEGA_ECOLI)

Last modified November 24, 2009. Version 90. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Protein aegA

Gene names

Name:	aegA
Synonyms:	yffG
Ordered Locus Names:	b2468, JW2452

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Cofactor	Binds 4 4Fe-4S clusters By similarity.
Sequence similarities	Contains 4 4Fe-4S ferredoxin-type domains.
Sequence caution	The sequence AAB46944.1 differs from that shown. Reason: Frameshift at position 602.

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Ontologies

Keywords
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 659

659

Protein aegA

PRO_0000170801

Regions

Domain

3 – 32

4Fe-4S ferredoxin-type 1

Domain

47 – 77

4Fe-4S ferredoxin-type 2

Domain

78 – 107

4Fe-4S ferredoxin-type 3

Domain

114 – 147

4Fe-4S ferredoxin-type 4

Sites

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 4 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 4 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 4 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 4 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

121

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

124

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

133

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

137

Iron-sulfur 1 (4Fe-4S) By similarity

Experimental info

Sequence conflict

488

L → R in AAB46944. Ref.1

Sequence conflict

513

V → A in AAB46944. Ref.1

Sequence conflict

600

W → M Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P37127-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: DD3ADF3E90218402
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FASTA

659

71,844

        10         20         30         40         50         60 
MNRFIMANSQ QCLGCHACEI ACVMAHNDEQ HVLSQHHFHP RITVIKHQQQ RSAVTCHHCE 

        70         80         90        100        110        120 
DAPCARSCPN GAISHVDDSI QVNQQKCIGC KSCVVACPFG TMQIVLTPVA AGKVKATAHK 

       130        140        150        160        170        180 
CDLCAGRENG PACVENCPAD ALQLVTDVAL SGMAKSRRLR TARQEHQPWH ASTAAQEMPV 

       190        200        210        220        230        240 
MSKVEQMQAT PARGEPDKLA IEARKTGFDE IYLPFRADQA QREASRCLKC GEHSVCEWTC 

       250        260        270        280        290        300 
PLHNHIPQWI ELVKAGNIDA AVELSHQTNT LPEITGRVCP QDRLCEGACT IRDEHGAVTI 

       310        320        330        340        350        360 
GNIERYISDQ ALAKGWRPDL SHVTKVDKRV AIIGAGPAGL ACADVLTRNG VGVTVYDRHP 

       370        380        390        400        410        420 
EIGGLLTFGI PSFKLDKSLL ARRREIFSAM GIHFELNCEV GKDVSLDSLL EQYDAVFVGV 

       430        440        450        460        470        480 
GTYRSMKAGL PNEDAPGVYD ALPFLIANTK QVMGLEELPE EPFINTAGLN VVVLGGGDTA 

       490        500        510        520        530        540 
MDCVRTALRH GASNVTCAYR RDEANMPGSK KEVKNAREEG ANFEFNVQPV ALELNEQGHV 

       550        560        570        580        590        600 
CGIRFLRTRL GEPDAQGRRR PVPVEGSEFV MPADAVIMAF GFNPHGMPWL ESHGVTVDKW 

       610        620        630        640        650 
GRIIADVESQ YRYQTTNPKI FAGGDAVRGA DLVVTAMAEG RHAAQGIIDW LGVKSVKSH

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the aegA locus of Escherichia coli: control of gene expression in response to anaerobiosis and nitrate." Cavicchioli R., Kolesnikow T., Gunsalus R.P. J. Bacteriol. 178:6968-6974(1996) [PubMed: 8955321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

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Cross-references

Sequence databases
	L34011 Genomic DNA. Translation: AAB46944.1. Frameshift. U00096 Genomic DNA. Translation: AAC75521.1. AP009048 Genomic DNA. Translation: BAA16342.2.
PIR	C65022.
RefSeq	AP_003053.1. NP_416963.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9060N.
STRING	P37127.
Genome annotation databases
GeneID	947383.
GenomeReviews	Gene locus JW2452 in contig AP009048_GR. Gene locus b2468 in contig U00096_GR.
KEGG	ecj:JW2452. eco:b2468.
Organism-specific databases
EchoBASE	EB2308.
EcoGene	EG12409. aegA.
CMR	Search...
Phylogenomic databases
HOGENOM	P37127.
OMA	VDTQHSG
Enzyme and pathway databases
BioCyc	EcoCyc:EG12409-MON. ECOL168927:B2468-MON.
Gene expression databases
Genevestigator	P37127.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR000759. Adrndx_reductase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR012285. Fum_reductase_C. IPR006006. Glut_synth_sub2. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF00037. Fer4. 1 hit. PF00070. Pyr_redox. 2 hits. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE.
TIGRFAMs	TIGR01318. gltD_gamma_fam. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 5 hits. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

AEGA_ECOLI

Accession

Primary (citable) accession number: P37127
Secondary accession number(s): P76560, P76970

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents