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Protein

NADPH--cytochrome P450 reductase

Gene
N/A
Organism
Vigna radiata var. radiata (Mung bean) (Phaseolus aureus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei217FMNUniRule annotation1
Binding sitei309NADPUniRule annotation1
Binding sitei549NADPUniRule annotation1
Binding sitei652NADPUniRule annotation1
Binding sitei690FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 93FMNUniRule annotation6
Nucleotide bindingi143 – 146FMNUniRule annotation4
Nucleotide bindingi182 – 191FMNUniRule annotation10
Nucleotide bindingi468 – 471FADUniRule annotation4
Nucleotide bindingi486 – 488FADUniRule annotation3
Nucleotide bindingi502 – 505FADUniRule annotation4
Nucleotide bindingi610 – 611NADPUniRule annotation2
Nucleotide bindingi616 – 620NADPUniRule annotation5

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
OrganismiVigna radiata var. radiata (Mung bean) (Phaseolus aureus)
Taxonomic identifieri3916 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25LumenalUniRule annotationAdd BLAST25
Transmembranei26 – 46HelicalUniRule annotationAdd BLAST21
Topological domaini47 – 690CytoplasmicUniRule annotationAdd BLAST644

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001676111 – 690NADPH--cytochrome P450 reductaseAdd BLAST690

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP37116.

Structurei

3D structure databases

ProteinModelPortaliP37116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 233Flavodoxin-likeUniRule annotationAdd BLAST152
Domaini289 – 535FAD-binding FR-typeUniRule annotationAdd BLAST247

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37116-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNSDLVRA VESFLGVSLG DSVSDSLLLI ATTSAAVVVG LLVFLWKKSS
60 70 80 90 100
DRSKEVKPVV VPRDLMMEEE EEVDVAAGKT KVTIFFGTQT GTAEGFAKAL
110 120 130 140 150
AEEIKARYEK AAVKVVDLDD YAADDDLYEE KLKKESLVFF MLATYGDGEP
160 170 180 190 200
IDNAARFYKW FTEGKDERGI WLQKLTYGVF GLGNRQYEHF NKIGKVVDEE
210 220 230 240 250
LAEQGAKRLV AVGLGDDDQS IEDDFSAWKE SLWSELDQLL RDEDDANTVS
260 270 280 290 300
TPYTAAILEY RVVIHDPTAA STYDNHSTVA NGNTEFDIHH PCRVNVAVQK
310 320 330 340 350
ELHKPESDRS CIHLEFDISG TSITYDTGDH VGVYAENCNE TVEETGKLLG
360 370 380 390 400
QNLDLFFSLH TDKDDGTSLG GSLLPPFPGP CSLRTALARY ADLLNPPRKA
410 420 430 440 450
ALLALATHAS EPSDERLKFL SSPQGKDEYS KWVVGSQRSL VEVMAEFPSA
460 470 480 490 500
KPPLGVFFAA IAPRLQPRYY SISSSPRFAP QRVHVTCALV YGPTPTGRIH
510 520 530 540 550
KGVCSTWMKN AIPSEKSQDC SSAPIFIRPS NFKLPVDHSI PIIMVGPGTG
560 570 580 590 600
LAPFRGFLQE RYALKEDGVQ LGPALLFFGC RNRQMDFIYE DELKSFVEQG
610 620 630 640 650
SLSELIVAFS REGAEKEYVQ HKMMDKAAHL WSLISQGGYL YVCGDAKGMA
660 670 680 690
RDVHRTLHSI VQEQENVDST KAEAIVKKLQ MDGRYLRDVW
Length:690
Mass (Da):76,506
Last modified:October 1, 1994 - v1
Checksum:i592966167E8561DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07843 mRNA. Translation: AAA34240.1.
RefSeqiNP_001304239.1. NM_001317310.1.

Genome annotation databases

GeneIDi106762177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07843 mRNA. Translation: AAA34240.1.
RefSeqiNP_001304239.1. NM_001317310.1.

3D structure databases

ProteinModelPortaliP37116.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP37116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi106762177.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCPR_VIGRR
AccessioniPrimary (citable) accession number: P37116
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 5, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.