Reviewed, UniProtKB/Swiss-Prot P37116 (NCPR_PHAAU)

Names and origin

Protein names	Recommended name:     NADPH--cytochrome P450 reductase       Short name=CPR       Short name=P450R     EC=1.6.2.4
Organism	Phaseolus aureus (Mung bean) (Vigna radiata)
Taxonomic identifier	3916 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme is required for electron transfer from NADP to cytochrome P450.
Catalytic activity	NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
Cofactor	FAD. FMN.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.
Post-translational modification	Glycosylated.
Sequence similarities	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	NADPH--cytochrome P450 reductase		PRO_0000167611
Regions
	Domain	82 – 233	152	Flavodoxin-like
	Domain	289 – 535	247	FAD-binding FR-type
	Nucleotide binding	179 – 210	32	FMN By similarity
	Nucleotide binding	325 – 336	12	FAD By similarity
	Nucleotide binding	465 – 475	11	FAD By similarity
	Nucleotide binding	544 – 562	19	NADP By similarity
	Nucleotide binding	639 – 654	16	NADP By similarity
Amino acid modifications
	Glycosylation	275	1	N-linked (GlcNAc...) Potential
	Glycosylation	339	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P37116-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 592966167E8561DE	FASTA	690	76,506
	10 20 30 40 50 60 MASNSDLVRA VESFLGVSLG DSVSDSLLLI ATTSAAVVVG LLVFLWKKSS DRSKEVKPVV 70 80 90 100 110 120 VPRDLMMEEE EEVDVAAGKT KVTIFFGTQT GTAEGFAKAL AEEIKARYEK AAVKVVDLDD 130 140 150 160 170 180 YAADDDLYEE KLKKESLVFF MLATYGDGEP IDNAARFYKW FTEGKDERGI WLQKLTYGVF 190 200 210 220 230 240 GLGNRQYEHF NKIGKVVDEE LAEQGAKRLV AVGLGDDDQS IEDDFSAWKE SLWSELDQLL 250 260 270 280 290 300 RDEDDANTVS TPYTAAILEY RVVIHDPTAA STYDNHSTVA NGNTEFDIHH PCRVNVAVQK 310 320 330 340 350 360 ELHKPESDRS CIHLEFDISG TSITYDTGDH VGVYAENCNE TVEETGKLLG QNLDLFFSLH 370 380 390 400 410 420 TDKDDGTSLG GSLLPPFPGP CSLRTALARY ADLLNPPRKA ALLALATHAS EPSDERLKFL 430 440 450 460 470 480 SSPQGKDEYS KWVVGSQRSL VEVMAEFPSA KPPLGVFFAA IAPRLQPRYY SISSSPRFAP 490 500 510 520 530 540 QRVHVTCALV YGPTPTGRIH KGVCSTWMKN AIPSEKSQDC SSAPIFIRPS NFKLPVDHSI 550 560 570 580 590 600 PIIMVGPGTG LAPFRGFLQE RYALKEDGVQ LGPALLFFGC RNRQMDFIYE DELKSFVEQG 610 620 630 640 650 660 SLSELIVAFS REGAEKEYVQ HKMMDKAAHL WSLISQGGYL YVCGDAKGMA RDVHRTLHSI 670 680 690 VQEQENVDST KAEAIVKKLQ MDGRYLRDVW

References

[1]

"Purification, characterization, and cDNA cloning of an NADPH-cytochrome P450 reductase from mung bean." Shet M.S., Sathasivan K., Arlotto M.A., Mehdy M.C., Estabrook R.W. Proc. Natl. Acad. Sci. U.S.A. 90:2890-2894(1993) [PubMed: 8464904] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Berken. Tissue: Seedling.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07843 mRNA. Translation: AAA34240.1.
3D structure databases
HSSP	HSSP built from PDB template 1AMO based on UniProtKB P00388.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.6.2.4. 29.
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR015702. NADPH_Cyt_P450_Rdtase. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
PANTHER	PTHR19384:SF17. NADPH_Cyt_Red. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCPR_PHAAU

Accession

Primary (citable) accession number: P37116

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	October 13, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)