P37115 (TCMO_VIGRR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trans-cinnamate 4-monooxygenase
EC=1.14.13.11

Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name=C4H
Short name=CA4H
Cytochrome P450 73
Cytochrome P450C4H

Gene names

Name:

CYP73A2

Organism

Vigna radiata var. radiata (Mung bean) (Phaseolus aureus)

Taxonomic identifier

3916 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Trans-cinnamate 4-monooxygenase		PRO_0000052250
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	40	1	V → I in clone PC4H-II.
Experimental info
	Sequence conflict	38	1	L → V AA sequence Ref.1
	Sequence conflict	498	1	V → I AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37115 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 563615D61D33BD95	FASTA	505	57,888
	10 20 30 40 50 60 MDLLLLEKTL LGLFLAAVVA IVVSKLRGKR FKLPPGPLPV PIFGNWLQVG DDLNHRNLTQ 70 80 90 100 110 120 LAKRFGDIFL LRMGQRNLVV VSSPDLAKEV LHTQGVEFGS RTRNVVFDIF TGEGQDMVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQYRHGW EAEAAAVVDD VRKNPDAAVS GLVIRRRLQL 190 200 210 220 230 240 MMYNNMYRIM FDRRFESEED PLFQRLKALN GERSRLAQSF EYNYGDFIPI LRPFLKGYLK 250 260 270 280 290 300 ICKEVKETRL KLFKDYFVDE RKNIGSTKST NNEGLKCAID HILDAEKKGE INEDNVLYIV 310 320 330 340 350 360 ENINVAAIET TLWSIEWGIA ELVNHPEIQQ KVRDEIDRVL GVGHQVTEPD IQKLPYLQAV 370 380 390 400 410 420 VKETLRLRMA IPLLVPHMNL HDAKLGGYDI PAESKILVNA WWLANNPAHW KKPEEFRPER 430 440 450 460 470 480 FFEEESHVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT LGRLVQNFEL LPPPGQSQID 490 500 TSEKGGQFSL HILKHSTVVA KPRSF

References

[1]

"Molecular cloning and sequencing of a cDNA encoding mung bean cytochrome P450 (P450C4H) possessing cinnamate 4-hydroxylase activity." Mizutani M., Ward E., Dimaio J., Ohta D., Ryals J., Sato R. Biochem. Biophys. Res. Commun. 190:875-880(1993) [PubMed: 8439336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-49; 255-262 AND 485-501. Tissue: Hypocotyl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07634 mRNA. Translation: AAA33755.1.
PIR	JC1458.
3D structure databases
ProteinModelPortal	P37115.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_VIGRR

Accession

Primary (citable) accession number: P37115

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 28, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program