P37114 (TCMO_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trans-cinnamate 4-monooxygenase
EC=1.14.13.11

Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name=C4H
Short name=CA4H
Cytochrome P450 73
Cytochrome P450C4H

Gene names

Name:

CYP73A3

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Induction	By fungal elicitor.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Trans-cinnamate 4-monooxygenase		PRO_0000052247
Sites
	Metal binding	448	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37114 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 5CD72A57FC031E0F	FASTA	506	58,280
	10 20 30 40 50 60 MDLLLLEKTL LALFIAATIA VTISKLRGKR FKLPPGPIPV PIFGYWLQVG DDLNHRNLTD 70 80 90 100 110 120 YAKRFGEIFL LRMGQRNLVV VSSPELAKEV LHTQCVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQYRYGW ESEAESVVND VKNNAEASVG GIVIRKRLQL 190 200 210 220 230 240 MMYNIMYRIM FDRRFESEED PLFVKLKALN GERSRLAQSF EYNYGDFIPI LRPFLKGYLK 250 260 270 280 290 300 VCKEVKDRRL QLFKDYFVDE RKKLESTKST TSNDGLKCAI DHILDAQKKG EINDDNVLYI 310 320 330 340 350 360 VENINVAAIE TTLWSIEWGI AELVNHQDIQ NKVREEMDRV LGPGHQVTEP DLHKLPYLQA 370 380 390 400 410 420 VIKETLRLRM AIPLLVPHMN LHDPKLNGFD IPAESKILVN AWWLPNNPAH WKKPEEFRPE 430 440 450 460 470 480 RFLEEESHVE ANGNDFRYLP FGVGRRSCPG IILALPILGI TIGRLVQNVE LLPPPGQSKI 490 500 DTSEKGGQFS LHILKHSTIV AKPRSF

References

[1]	"Stress responses in alfalfa (Medicago sativa L.). XVIII: molecular cloning and expression of the elicitor-inducible cinnamic acid 4-hydroxylase cytochrome P450." Fahrendorf T., Dixon R.A. Arch. Biochem. Biophys. 305:509-515(1993) [PubMed: 8373188] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11046 mRNA. No translation available.
PIR	S36878.
3D structure databases
ProteinModelPortal	P37114.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_MEDSA

Accession

Primary (citable) accession number: P37114

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 28, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program